FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTGCAGCTTCGGCCGCTGGCCCTCAGGCCAGGCGTCGAGGAAGCGGC48.16326530612245No Hit
CCGGTTACCTGCCAGAAAGGTTTTCACATCGAGGTCTAAGCGAGCTACCT24.081632653061225No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC24.081632653061225Clontech Universal Primer Mix Long (96% over 26bp)
ACCAATAGTAACAGTTAATTTTAATAGCAAAGAATACAATTTCCTTAGGT24.081632653061225No Hit
GTGCGCCTGCCCGCTTTAGCCCATTTGGCCGCAACGTCGAGCAACAGGGT24.081632653061225No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12.0408163265306123Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT12.0408163265306123No Hit
ACCTATAACTGGTCCCAGCATCCCTGTGGCACCTTTGGATTCCCTGAGCT12.0408163265306123No Hit
GCTGTGTCACTCCCCGAGTTCCATGTCCAGAGCCGCATCCACCAACGTCA12.0408163265306123No Hit
GCAAACATTCTTAACATGTCTTCTGGAAAATGTACCTACTTTGGTTTCTC12.0408163265306123No Hit
CTCTCTTACTACATCTGTTTTTGTTCTACCAGACGAGAGTGCAGTATTTC12.0408163265306123No Hit
AGGGAGGGGTGCAGGCTGAAGCAGCGCCTCAACAGCCGGGACATGTAGGC12.0408163265306123No Hit
GGCCAAGGGGACCTGGGCGGCAGGGGCTGGAGCTCCGGCGGGCCAGCGCC12.0408163265306123No Hit
CGCACCAGCCAAGGCAAACTCGTGGCTTGTACCCCTAGCGATAGCAGAAC12.0408163265306123No Hit
GTGTTGGTTTGGCTGGTCTCGAACTCCTGACCTCAAATTATCCCGCCCTC12.0408163265306123No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12.0408163265306123Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTCGCGCTAGCCGGAGGACGCGCTGCTGGCCCTCTGCGACGAGCTGG12.0408163265306123No Hit
CCCGACCTGCGCTGGCTTGCCGGCCTGGCCTGCACCGCCATCCTGCTGCA12.0408163265306123No Hit
GAATGAGGAGGGCCGCGACGACGCGGACGACGGTGGGGTCGAGATTGAGG12.0408163265306123No Hit
GCTCATACGCGCCGCCGCGTGAGAGCGCACGTTGGTAGGCTAGACGTGTA12.0408163265306123No Hit
CCCCAAGGGCGACGCGGATCTCTTGAAACGCATGTACGTCGAAGCCAAGC12.0408163265306123No Hit
GAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTG12.0408163265306123No Hit
GGTTGGTACGTTCGCGCAGAACGACACGCGGATCGTTGCGTAGCCCCCAA12.0408163265306123No Hit
GCGAATTGCCGTCGCGGTCGGCGTACTCGACCTCGAAGAGCTCGAGCCCT12.0408163265306123No Hit
TGCTTGTTTCTAGCAAGTTGGTGGTTATAACTGTCAATTGAAAGAACAGG12.0408163265306123No Hit
GGCAGAAGTGAAGTGGCAAGGGAGGTCATCTGTCATGAGGTTGGCTTTCA12.0408163265306123No Hit
GCGCAGGGCAGAGCGGCTGGCGCCGCCGGAGCGCGGAGCCACGACCCTCC12.0408163265306123No Hit
GGATCGGGGATTGCAATTATTCCCCATCACGAATGGGGTTCAACGGGTTA12.0408163265306123No Hit
TCCGGAGGAGGCTGGGGAGGGATTTCCGCAGTCGGCCCTGGAAGCCCTGG12.0408163265306123No Hit
GGCCTGGCCGACCCCTACCGGAGTCTCAAAGCGCCTGGGGCTCGCGGACC12.0408163265306123No Hit
GCATTGCGGTATGCCCGCACCCTTCTTACTGGGCAACTTAGGCAGCTAAT12.0408163265306123No Hit
ATCAAACAGCTTACAACCAAATTCTAGGACTTACACCAAGGATCGTCGTA12.0408163265306123No Hit
CCACGCGCCCCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCCGGGG12.0408163265306123No Hit
GCTTCCCATGTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGT12.0408163265306123Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG12.0408163265306123Clontech Universal Primer Mix Long (96% over 26bp)
GGTTATGGCGTGCAACGCTGGACAAATCGAGGGTATAAGCACACGTCTCG12.0408163265306123No Hit
GTGGGGAGCGCCTCTGCCCCACCGCCCCGTCTGAGATATGAAGAGCACCT12.0408163265306123No Hit
CATGCAAGTCGAACGGTGTCTTCGGACATAGTGGCGGACGGGTGAGTAAC12.0408163265306123No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT12.0408163265306123Clontech Universal Primer Mix Long (96% over 26bp)
ATGCCATGTAAGACATGCCTTTGTTCCTCCTTCACCTTCCGCCATGATTG12.0408163265306123No Hit
TAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACAT12.0408163265306123No Hit
GCGTCGTGCAGGTTCGGGAGGTCGTGGCGGGCAAGAAGGTCGGCCGGCAG12.0408163265306123No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)