FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49
Sequences flagged as poor quality0
Sequence length140
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGACGACCTCCGGTTCTCGGCCGCCGACGTGCTGTGGATCGTGGAGC48.16326530612245No Hit
CTTCATCAGCACCATTTTTTTTGGCATGCTCGATCATAGCCTCCTTAGAG24.081632653061225No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC24.081632653061225Clontech Universal Primer Mix Long (96% over 26bp)
GCCAGGAGTCGGAAAATCGACCCTGTTGCTCGACGTTGCGGCCAAATGGG24.081632653061225No Hit
CTGTTTTAATCTATCAATAATACTTGTTACTAACTGAAACTATTTTGTTT24.081632653061225No Hit
GTCTTGAACTCCTGACCTTGTGATCTGCCCACCTTGGCCTCCCAAAGTGC12.0408163265306123No Hit
GTCCTGTGGATTTGGGGCATCATCACCAAGAAGAAGGCACAGTAATCACC12.0408163265306123No Hit
ACCTGGTACAGAAGTCGAAACCACAGCCAAGATTGAGATTAAAGCGCGAT12.0408163265306123No Hit
TAGCAGAGCAGCTCCCTCGCTGCGATCTATTGAAAGTCAAACCCTTGTGT12.0408163265306123No Hit
TCGTCGATCGACTTGATCTCGCCCGAGAGAAATCCGCGGCACAGGGGGCT12.0408163265306123No Hit
CTAAATATTCGATTACCTCGGTCGGAATTAGAAATCCTAGAGAAATACTG12.0408163265306123No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG12.0408163265306123Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAGGGCCACGCGGTCCACCAAGACACTCAAGGTGGTGGTGGC12.0408163265306123No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC12.0408163265306123Clontech SMART CDS Primer II A (96% over 26bp)
CCGGAGGCGCCTCGCAGCGGTCGGGGAACGCGGGGTCCCCCTGCTGCGGG12.0408163265306123No Hit
GGCGTACTCCATGAACAGCGCGTCGATGTTGATTACGTGCCGCTGGATCA12.0408163265306123No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTAGCGTTTATACCACT12.0408163265306123No Hit
GAGGAAGGGTTCCGGGGCCTCTGGAAAGGGACCTCTCCCAATGTTGCTCG12.0408163265306123No Hit
GTTAACTGAATTCTACTGAAAGCTAGAGCTCTGTTGAGTAATCTTTTAAA12.0408163265306123No Hit
AGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAA12.0408163265306123No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG12.0408163265306123Clontech Universal Primer Mix Long (96% over 26bp)
CACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTA12.0408163265306123No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGC12.0408163265306123No Hit
GCATAGGGATTTTGGAAGCAGCTGCTGGTGGCTTGGGACATCAGTGGGGC12.0408163265306123No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG12.0408163265306123Clontech Universal Primer Mix Long (96% over 26bp)
GGGCAGAGGGGCTCCTCACCTCCCAGACGGGGCTGCTGGGCAGAGGGCCC12.0408163265306123No Hit
CCCAGGGGAGCTGCCCAATAAGGACCGATCTCAAAGCACCCCCAGAGAGG12.0408163265306123No Hit
TACCCCACCAACTAGCTAATCAGACGCGAGCTCTTCAATTGGCAATTTAT12.0408163265306123No Hit
GGCCAGGCGCTTGCGGGCCCTGATCCACCGCGCCCTAGACGCCGCCGGCC12.0408163265306123No Hit
GGGCTTGGAATCGGCCGGGTCGCTCGCGGTGGTGCGCAGGGCCATCGTGC12.0408163265306123No Hit
GGAGTCTCCGCTTCCAGTCGCATAACTTTGTTAAGTTTTTGGCAATATGC12.0408163265306123No Hit
GGTGTAGGCGTCGTCGTGATCACGCCGTTGCTGTTGGGGACAATCAGGCG12.0408163265306123No Hit
ATAAAAGGGAAGGGATTGACCCCACTCCCTATTACTGGTATACGGATCAG12.0408163265306123No Hit
GTCTCGATCTCCTGACCTTGTGATCTGCCTGCCTCGGCCTCCCAAAGTGC12.0408163265306123No Hit
TATCAACGCAGAGTACATGGGCGTCTTCCTGCACGAAGGAGAAGTCCTGC12.0408163265306123No Hit
CCCTCGTCGGTTGGTCCCGCTACCGGCCGGCCTGGGATCCTGCGAAGGAG12.0408163265306123No Hit
CACTTGAACAGATAGTTCGAGACAATAATTGTTAACAAAACTTCATTAGA12.0408163265306123No Hit
GAATTGATAGCATTCAGCAATGCGCAAGTAAAGTTCCCAGTCTTCACAAC12.0408163265306123No Hit
GTGTCGGCGCAGATAAGCTTGGATGAGAGTCGTCCGAGGCTTAGGTGCTT12.0408163265306123No Hit
GTCCAGCGTGAGGCGGCGCGGCAGCGCCTGCAGGCTCAGCACGCCCAGCA12.0408163265306123No Hit
ATTAACCACTCAGCATTAGGGGCAAGGCTCTCTCGATTTGGAACTTCATA12.0408163265306123No Hit
CTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAG12.0408163265306123No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)