FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143
Sequences flagged as poor quality0
Sequence length140
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG42.797202797202797Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACC42.797202797202797No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA32.097902097902098Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT32.097902097902098Clontech SMART CDS Primer II A (100% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC32.097902097902098Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG21.3986013986013985Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGGAAGCAGTGGTATCAACCT21.3986013986013985Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATACAC21.3986013986013985Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA21.3986013986013985No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT21.3986013986013985Clontech Universal Primer Mix Long (100% over 23bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC21.3986013986013985Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGCG21.3986013986013985Clontech Universal Primer Mix Long (96% over 26bp)
GCTGAGTGTCCCGCGGGGCCCGAAGCGTTACTTTGAAAAAATTAGAGTGT21.3986013986013985No Hit
GTGATCATCATCGAATGGACCCGAATGGAATCATCCTCCAACGGAAGCTA21.3986013986013985No Hit
GGAAGCAGTGGTATCAACGCAGAATACATGGGAAGCAGTGGTATCAACGT21.3986013986013985Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAGTTCTGTACTTTGCAAAATTGCTAATGTCCCATGCCATTTGTGCTCAC21.3986013986013985No Hit
GGTATATGACTTGCAAATATCTTCTCCCATTCTGTGTGTTCCTTTTTGAC21.3986013986013985No Hit
CGGGTATGTCACCGGGGCCGCCTGCGCCATGCGTCCGCCCGGGACGTTGG21.3986013986013985No Hit
GATCGGAAGAGCGTCGTGTAGGAAAGTGTAGACCGGTGGTCGCCTATTCC21.3986013986013985Illumina Single End PCR Primer 1 (96% over 25bp)
TTCCCAGCAACTCCAGTCCTGCATCATGAGATCGGAAGAGCGTCGTGTAG10.6993006993006993Illumina Single End PCR Primer 1 (100% over 22bp)
CGGTGGCGCCGTTCACCCATGTACTCTGCGTTGATACCACTGCTTCCCAT10.6993006993006993Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAACACTGCGTGGAAAC10.6993006993006993No Hit
TTCCCTACACGACGCTCTTGCCTCAGATTCTGTCGGTACCAGAGATCGGA10.6993006993006993Illumina Single End PCR Primer 1 (95% over 21bp)
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACTCTGCGTTTA10.6993006993006993No Hit
TTTTCTTTCTTCCTTCCTTTCTTCCTTCCTTCCTTCCATCCTGCCTTCCT10.6993006993006993No Hit
GGATGAGAATGTGAATAGATTTATGTCCATCCATTACCTGTCTCTTATAC10.6993006993006993No Hit
GAGATCTACACTCTTTCCCTACACGACGCTCTTCCGACTGTCTCTTATAC10.6993006993006993Illumina Single End PCR Primer 1 (97% over 37bp)
CAATAAACAAAGACTACATTTGAAGCAAACTTAAAAAAAAACACAACAAC10.6993006993006993No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA10.6993006993006993No Hit
CCTCATTCAGGAACTGGAGGCCCAGATCGGAAGAGCGCTGTCTCTTATAC10.6993006993006993No Hit
GGTTGTTGGCGGTGGCCGCGGCCTTGGGGATGTCGTTCCCGCCTGTCTCT10.6993006993006993No Hit
AGTCGCTACTGAAACAGTTGTTAGGGAGGCCAGTGTTAGTGAGACCTGGC10.6993006993006993No Hit
CCTACACGACACTTGGGACAGCGTCGTGTAGGAAAGAGTGTAACTCGTGG10.6993006993006993No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.6993006993006993Clontech Universal Primer Mix Long (96% over 26bp)
AAGCATTCCCACGCGGGCCGGCAACTGTTGCTCGACCTAGTTGCCGGCCC10.6993006993006993No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCACCGTATAAAA10.6993006993006993No Hit
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA10.6993006993006993Clontech Universal Primer Mix Long (95% over 22bp)
GCTGGTACCAGTAGACAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGGT10.6993006993006993Illumina Single End PCR Primer 1 (96% over 28bp)
TATCAACGCAGAGTACATGGGATGATACGGCGCCACCTGTCTCTTATACA10.6993006993006993No Hit
ACCGCGAGCGCGGTAGTGCGGTTGTCGCCCGTCATCATCACCACGCGAAC10.6993006993006993No Hit
GGAGGGCGCGTGCCCCGCCGCGCGCCGGGACCGGGGTCCGGTGCGGAGTG10.6993006993006993No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.6993006993006993Clontech Universal Primer Mix Long (96% over 26bp)
GCAGCTCCGTGTAGGAAGAGTGAATCTGTGTCCCGTACACCCATGTACCT10.6993006993006993No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTT10.6993006993006993Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTC10.6993006993006993Clontech Universal Primer Mix Long (96% over 26bp)
CGGCCAGGTAGTAGTCAACGACCTCGGCCTGGGTCGTGCCTGTCGCCGGG10.6993006993006993No Hit
CTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAATTA10.6993006993006993No Hit
TGATGGTGGGCGCCTGTAATCCCAGCCACTCCAGAGGCTGAGGCAGGAGA10.6993006993006993No Hit
GATAAACAACAATAGGAACACAACAGTATCTTTTCTTCTTCCTGACTCAA10.6993006993006993No Hit
GGAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAATTTA10.6993006993006993Clontech Universal Primer Mix Long (95% over 23bp)
ACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAG10.6993006993006993No Hit
TCCCCAGCAACTCCAGTCCTTGCATCATGTGATCGGAAGAGTCGTGTAGG10.6993006993006993No Hit
CTATCAAATAAAAAAAAATCCGAACCAAAGATAACAAAGCATAATCAAAG10.6993006993006993No Hit
GATCTACACTCTTCCCTACACGACGCTTGCCTCAGATTCTGTCGGTACCA10.6993006993006993No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACTCTGGGGTTATACCAC10.6993006993006993No Hit
ACCAGTTCCATGAAAGACCATTTTTCCACAGACTGCGGGGCTGGGGAGTG10.6993006993006993No Hit
AAAAAAGTACACTGCGTTGATACCACAGCTTCCCATGTACTCTGCGTTGA10.6993006993006993Clontech SMART CDS Primer II A (95% over 21bp)
GGCGGGAACCCTTTGTTCTGGAAGTCCTGGGAGTTGCAGACCAGATCACC10.6993006993006993No Hit
GGGAGGAACTTGATCCAGCAGAGCAGCACCACCTCGGCCAGGAAGAGTAG10.6993006993006993No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTAAGCGGTGAAACCACAGCATA10.6993006993006993No Hit
GATCTACACTCTTTCCCTACACGACCCTCTTCCGATCTCTGTCCCAAGTT10.6993006993006993Illumina Single End PCR Primer 1 (97% over 38bp)
GTACATTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG10.6993006993006993Clontech Universal Primer Mix Long (96% over 26bp)
TTTTCTTTCTTCCTTCCTTTCTTCCTTCCTTCCTTCCTTCCTGCCTTCCT10.6993006993006993No Hit
ACGCTCTTCCGATCTATCCCTTTTTTGGCCTGTTGGTACCAAAAAAGGGA10.6993006993006993No Hit
ATCTACACTCTTTCCCTACACGACGCTCTTCCTGTCTCTTATACACATCT10.6993006993006993Illumina Single End PCR Primer 1 (96% over 32bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACTCTGCGTTTATACCA10.6993006993006993No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.6993006993006993Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTAGC10.6993006993006993Clontech Universal Primer Mix Long (96% over 26bp)
CTGCAGATACAATGACGGAATCAGGAGCCAGATGCAGTGGCTCCTGATTC10.6993006993006993No Hit
GCTTGGGAGAAATGCACAGATTCTGGGTGCCATGTGCATTTGTTTTGGGA10.6993006993006993No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACTCTGCGTTTTTACC10.6993006993006993No Hit
ATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTG10.6993006993006993No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT10.6993006993006993Clontech Universal Primer Mix Long (96% over 26bp)
GGTCCTGAACTCCTGACCTCAGGTGATCTGCCTGCCTCGGCCTCCCAAAG10.6993006993006993No Hit
ACTTGCAACAGCAGACGCGCCTTCAGCTGCTTCTCTTTCAGTTTCGCCGC10.6993006993006993No Hit
TCCGATCTGCCGATTAACGGCGGTTAACGTGTGAGCACCCAGGGAAGGGC10.6993006993006993No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCCTGTC10.6993006993006993Clontech Universal Primer Mix Long (96% over 28bp)
AGTCGCTACTGAAACAGTTGTTAGGGAGGCCTGTGTTAGTGAGACCTGGC10.6993006993006993No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTGTTTTTTTTTTTGTA10.6993006993006993No Hit
CACGTAAGATTAACCATCATGAGGAGTATAATTTTTCTTCATGATGGACA10.6993006993006993No Hit
ATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGG10.6993006993006993Clontech Universal Primer Mix Long (96% over 26bp)
CCTCAAGGTTGAGGCCGCCATTGGAAGATGAAAAATTGCACTCCCTTGGT10.6993006993006993No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACTATGCTTTGATACCACT10.6993006993006993No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTCATGGGAAGCAGTGGTAT10.6993006993006993Clontech Universal Primer Mix Long (100% over 23bp)
TATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGGGATT10.6993006993006993No Hit
TACACATGCTAATAAACAAAAAGGAAATAACTGTTTTCAAGAAATAAAAC10.6993006993006993No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAATATGCGTTGAAACCA10.6993006993006993No Hit
TTGTCTGTCCATTGTTAAATGGTTGGGATTTATTGGGAGGATTTCTGTCT10.6993006993006993No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.6993006993006993Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAATGGGGTTT10.6993006993006993No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTT10.6993006993006993No Hit
CCCTTGTTCTGGGTATTCAGTGTATAGGTCCTGTGAACATAGAAAACCTG10.6993006993006993No Hit
CAAATAATATCGTGTTTATATCGGAACATAAAACTCAGTGTGGAGCTAGG10.6993006993006993No Hit
GAGGAAGTAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.6993006993006993No Hit
ATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTT10.6993006993006993No Hit
GACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCTGCTGAGAT10.6993006993006993Illumina Single End PCR Primer 1 (97% over 43bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAGAAAAAAAAAAAAAAAAAAA10.6993006993006993Clontech Universal Primer Mix Long (95% over 22bp)
CATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATAC10.6993006993006993Clontech Universal Primer Mix Long (96% over 26bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAAATGCGTTTAAACCAC10.6993006993006993No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTT10.6993006993006993No Hit
CATCATTCCAGAGCCCCAGTGGAAACTTCTTTCAGATTCTGCGGGGACTG10.6993006993006993No Hit
GCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAATATTAGAGTG10.6993006993006993No Hit
CTACTTAGGCCATTTAAAGCTGGTGCAGCCTATAAAATTTTGTTACTGAT10.6993006993006993No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.6993006993006993Clontech Universal Primer Mix Long (96% over 26bp)
CCCCGCCGCGCGCCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTG10.6993006993006993No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.6993006993006993Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTACTCTGCGTTGATACCACTGCTTGCCATGTACTCTGCGTTGATACC10.6993006993006993Clontech Universal Primer Mix Long (96% over 28bp)
TCTTGAGCGCGCGTAAACCAGCTTCTCCGCGCTCTTCATAGACCTTCAGC10.6993006993006993No Hit
CTTCTGATGTTCGGATATGTTCGGAGTTCCTTCCTTCTGGTGGGTTCCTG10.6993006993006993No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAACTGTC10.6993006993006993Clontech Universal Primer Mix Long (95% over 21bp)
ATCTTGGCTCACCGCAACCTCCACCTCCCGGGTTCAAGTGATTCTCCTGC10.6993006993006993No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTA10.6993006993006993No Hit
AAACTGAGTTGTTTTCTGTTAAAAAAAAAAAAACAGGGTGGGGTTGGGGG10.6993006993006993No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.6993006993006993Clontech Universal Primer Mix Long (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.6993006993006993No Hit
ATACCACTGCTTCCCATGTACTCTGAGTTGATACCACTGCTTCCCATGTA10.6993006993006993Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAGCATTCCCACGCGGGCCGGCAACTGTTGCTCGACCAAGTTGCCGGCCC10.6993006993006993No Hit

[FAIL]Adapter Content

Adapter graph