FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG42.797202797202797Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT42.797202797202797Clontech SMART CDS Primer II A (100% over 26bp)
CTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC42.797202797202797No Hit
TTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTC32.097902097902098No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT32.097902097902098Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG21.3986013986013985Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT21.3986013986013985Clontech Universal Primer Mix Long (96% over 26bp)
ATTCGATTCCATTCTATGACGATTCCATTCATTTCCATCTGATGATGATT21.3986013986013985No Hit
GGTTGCAGTGATCCAAGATCACACCACTTCACTCCAGCTTGGGCAACAGA21.3986013986013985No Hit
GTGGTCGCCCGAGGCGGCGAGCGCGACGGCCTCGCGGAACACCTCAACAT21.3986013986013985No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA21.3986013986013985Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21.3986013986013985Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT21.3986013986013985Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTCTGTCTCTTATACAC21.3986013986013985Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG21.3986013986013985Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTTGATACCACTGCTTCCCCATGTACTCTGCGTTGATACCACTGCTTCCT21.3986013986013985Clontech SMART CDS Primer II A (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAATAGGCGACCACCGGT21.3986013986013985Clontech Universal Primer Mix Long (96% over 26bp)
TGCTCTGCGTGCCGGGCAGCGGCCGCCCGCCGAAGGGTGGGGCGCCGATC21.3986013986013985No Hit
ATATATCATATATCTCAATATAAGAGCTGAAACTATTATCACTCTTAGAA21.3986013986013985No Hit
GAACACAGTAGGTACTGGATAACATGTGTTGAATCAATCAACGAATCTGA21.3986013986013985No Hit
CTATCATTCCGCGTTTGGGCTATTATGAAGAAACTATCATGAGCATCCAT21.3986013986013985No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTT21.3986013986013985Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGTGAGGCGACCCAGATATAAACACATTACCAACACCACCC10.6993006993006993No Hit
AGATCGGAAGAGCGTCGTGTAGGAAAAGTGTAGATCGGCCCGCACCCATG10.6993006993006993Illumina Single End PCR Primer 1 (96% over 28bp)
AGTTTATATAGTATTTAAACTAATTCAGATGAAGTTTTATTTCTTGAAAA10.6993006993006993No Hit
GAGTACATGGCAAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGG10.6993006993006993Clontech Universal Primer Mix Long (100% over 26bp)
GCCTGGGCATTCCCTTTGCCCAAATCCTTGTCCTACGGAGCAGAGAGGCA10.6993006993006993No Hit
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTGTCTCTTATACACATCT10.6993006993006993Illumina Single End PCR Primer 1 (96% over 33bp)
TTCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTA10.6993006993006993Illumina Single End PCR Primer 1 (100% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAGCGTTTATACCAA10.6993006993006993No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTCTTTTTTAAAAAAAAAAAAAAAA10.6993006993006993No Hit
ATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCT10.6993006993006993No Hit
GCCTGAGCTCAAGTGATCCGCCATCCTCTGCCTCCCAACTCCTGATCTCA10.6993006993006993No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGA10.6993006993006993Clontech Universal Primer Mix Long (96% over 26bp)
TGTCCGGGCCGGGTGAGGTTTCCCGTGTTGAGTCAAATTAAGCCGCAGGC10.6993006993006993No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTATTGAGTTTAAACAACACATAC10.6993006993006993No Hit
GCGCCGCGGGGCCCCGGGTGGGCCCCGGTATGCCCGGCAGGCGGGGGGGC10.6993006993006993No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAC10.6993006993006993No Hit
TCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCCTGTCTCTTATAC10.6993006993006993Illumina Single End PCR Primer 1 (97% over 37bp)
GGGCCCTTCCCTGGGTGCTCACACGTTAACCGCCGTTAATCGGCAGATCG10.6993006993006993No Hit
GTACATGGGTGATGGCGAACCGAGACTACACCTTTCCCTACACGACGCTC10.6993006993006993No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAACTGTCTCTT10.6993006993006993Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGG10.6993006993006993No Hit
CCTCCCCGGGGCGGGTGCTGTACCCGGCGACAGGCACGACCCAGGCCGAG10.6993006993006993No Hit
AAGCAGAAGAATCGTTTGAGCCTGGGAGGTGGAGGCTGCAGTGAGCCGAC10.6993006993006993No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.6993006993006993No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTC10.6993006993006993Clontech Universal Primer Mix Long (96% over 26bp)
GCGGGAACGACATCCCCAAGGCCGCGGCCACCGCCAACAACCCTGTCTCT10.6993006993006993No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAACAGCGTTTAAACAAC10.6993006993006993No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTC10.6993006993006993Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACTCT10.6993006993006993No Hit
TATCAACGCAGAGTACATGGTGATACCGACCACCGAGATCTACACTCTTT10.6993006993006993Illumina Single End PCR Primer 1 (96% over 25bp)
ATGTTTAGCATGGTGTAGTTGATTGAGAAGATTTTCGTTTTGCTGGTGGA10.6993006993006993No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTCCAAAGTAAAAAGTGTTTTTT10.6993006993006993No Hit
GTAATGGATGGACATAAATCTATTCACATTCTCATCCCTGTCTCTTATAC10.6993006993006993No Hit
GTACATGGGTGTACGGGACACAGATTCACTCTTCCTACACGGAGCTGCCT10.6993006993006993No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.6993006993006993Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGAC10.6993006993006993No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTGGGTGTTAAAAAAGAT10.6993006993006993No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTGTTTGCGTTTTAACCACGGGTAC10.6993006993006993No Hit
CCTAAAGGGCATTCATGCCCACGTTGGCAGTTTCAGGAAGTTTTATTGCT10.6993006993006993No Hit
CCCCTACATCGAGCTGGCCTGGGGCTTCTCCACCGTGCTTGGCATCCTAC10.6993006993006993No Hit
GGTGAAACCCCATGTTGTCTCTACCAAAAATACAAAAAAAAAAAAAAAAA10.6993006993006993No Hit
AACGCAGAGTACATGGGAGATTGCGACCACAGATCTCATCTTTCCCTACA10.6993006993006993No Hit
TAACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.6993006993006993Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCACACTGTGGAAGCTTTGTTCTTTCACTCTTTGCAATAAATCTTGC10.6993006993006993No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT10.6993006993006993Clontech SMART CDS Primer II A (100% over 26bp)
GGCTCGCCGGACTGATTGGGATCGCTGATCCGATCAAGGAGAGCACGCCG10.6993006993006993No Hit
GTGGTATCAACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACGGT10.6993006993006993No Hit
AAAAGATGGAGAAATGATGGAAGAGCATTCATTCTGGGGCAGGGCACTGA10.6993006993006993No Hit
ATCTACATCTTTCCCTACACGACGCTCTTGCCTCAGATTCTGTCGGTACC10.6993006993006993Illumina Single End Sequencing Primer (95% over 24bp)
GCTACCTCTCTCAAGTCCCAAGGCAGAATTCCTCATTGTAGTAGAAAGGC10.6993006993006993No Hit
AAATTAGCCGGGCATGGTGGCTCATGCCTGCAATCACACTACTCAGGAGG10.6993006993006993No Hit
GCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAG10.6993006993006993No Hit
GTACATGGGAAACCACCACCGAATCACTCTTTCCCTACACGACGCTCTTC10.6993006993006993Illumina Single End Sequencing Primer (96% over 27bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.6993006993006993Clontech Universal Primer Mix Long (96% over 26bp)
CTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCTGCAGAACTTGG10.6993006993006993Illumina Single End PCR Primer 1 (100% over 30bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.6993006993006993Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAATGTG10.6993006993006993No Hit
GAGTACATGGGTGTACGCGACCACCGAGATCTACACTCTTTCCCTACACG10.6993006993006993Illumina Single End PCR Primer 1 (100% over 34bp)
CATTTGGGCTCTGGACTCCACATACCTATCAGTGAGGTGAGGCCAAGGCA10.6993006993006993No Hit
ACGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAAAGGCGTTTATAA10.6993006993006993No Hit
GTGGCGCCGTATCATCCCATGTACTCTGCGTTGATACTGTCTCTTATACA10.6993006993006993No Hit
CTGCAATCTCTGCCTCGCAGGTTCAAGCGATTCTCCTGCCTCAGCCTCTG10.6993006993006993No Hit
ACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA10.6993006993006993No Hit
TTTTTTTTTTTTTTTTGTGCGTTGATACCACTGCTTCCCATGTACCTGTC10.6993006993006993Clontech Universal Primer Mix Long (95% over 21bp)
GCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAACTGCGTTTTT10.6993006993006993No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGTAGTGGTA10.6993006993006993Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAGTAATCTGCGTTT10.6993006993006993No Hit
GGAACTGAATTTTAAATTTTATTTCATTTTAATTAAATGTAATGAAAACG10.6993006993006993No Hit
GGGAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAGTAC10.6993006993006993Clontech Universal Primer Mix Long (95% over 24bp)
GCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGT10.6993006993006993No Hit
GGCAATGATAGGTCAGAAAAGGCTCCTAGAGTTGGTGACATTTGAGCTGG10.6993006993006993No Hit
CTGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACT10.6993006993006993Clontech SMART CDS Primer II A (100% over 26bp)
ACATGGAATACGCGCCACGAGTTACACTCTTTCCTACACGACGCTGTCCC10.6993006993006993No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.6993006993006993Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAGCGGACACGAGATTACACACTTTACTAAACGACGCGCGTT10.6993006993006993No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10.6993006993006993Clontech Universal Primer Mix Long (96% over 26bp)
TTGCTGACAGTTAATATTTTCTTTTTATGTATTGTATTTCCATAAGTTAG10.6993006993006993No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAGGGCGTTTAAA10.6993006993006993No Hit
CTGCACGGGTGGGGAAACCTCACAATCACCAGGGAAGGCAAGAAGGAACA10.6993006993006993No Hit
ACGCAGAGTACATGGGAAGCATTGGTATCAACGCAGAGTACATGGGAAGC10.6993006993006993Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC10.6993006993006993No Hit
GTATCAACGCAGAGTACATGGGATAACGCGACACCGAGATCTACACTCTT10.6993006993006993No Hit
GTACTTTTTTTTTTTTTTTTTTTCTGCGTTTTTACCAATGCTTCCCATGT10.6993006993006993No Hit
CGCTCTTCCGATCTGGGCCTCCAGTTCCTGAATGAGGCTGTCTCTTATAC10.6993006993006993No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTT10.6993006993006993No Hit
CATCGTCGCCGTTACATTCCGGAGCTGACCTCCCGTAACTTTAACATTCG10.6993006993006993No Hit
ATCAACGCAGAGTACATGGGTGATACGCGACCCCGAGATCTCACTGTCTA10.6993006993006993No Hit
ACTCTGCGTTGATACCACTGCTACCCATGTACTCTGCGTTGATACCACTG10.6993006993006993Clontech SMART CDS Primer II A (95% over 23bp)
CACTCCACGTGAGGACTAAAATAAGTGTTGGATTCTAACTTCAAGCCTGC10.6993006993006993No Hit
GTACATGGGAGCGGACACGAGAATACACTCATTCCAACACGAAGCGCGTT10.6993006993006993No Hit
GTACATGGGAAGCAGTGGTATCAACTCAGAGTACATGGGAAGCAGTGGTA10.6993006993006993Clontech SMARTer II A Oligonucleotide (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)