FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33
Sequences flagged as poor quality0
Sequence length140
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGCACAGTCAGCCTGAGATGTCCCTGTAATCATGTTTTTGATAAAG39.090909090909092No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA26.0606060606060606Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCGCCAGCCGGCGGCGCCGCGCCGCGCAGCTCCTCAGGCGTCCGTCCTCA26.0606060606060606No Hit
GTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGC26.0606060606060606No Hit
GTTCCTGCTTTCACAGAATTATTCCAGGGTTTATGTGTCAGGGTGGTGAC26.0606060606060606No Hit
TAACCAAAGTTAATCTTAATTGCAATTTGACTCCGTTTCCTTGGTAGGGA26.0606060606060606No Hit
GGTAACTCTTTAGAAACTGCAAACTGTTTTTAAAAGTGGCTGCGCTATTT13.0303030303030303No Hit
GTATCAACGCAGAGTACATGGGGCATAGAAAAAGGCAGGGCAAATAAAAG13.0303030303030303No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT13.0303030303030303Clontech Universal Primer Mix Long (96% over 26bp)
GCTCTTTATAGATATCACGAGTGTACTTGGTAGAGGTGACCTCGTAAACG13.0303030303030303No Hit
ACCTTAGACACATGCCATTAAATGAATAGTCTGAATGACTATGACATCGT13.0303030303030303No Hit
GTGTAGGGATTACAGGCATGAGCCATTGCGCCTGGCCCTGAATCATTTTT13.0303030303030303No Hit
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTT13.0303030303030303No Hit
GCCGAGCCCGAAGTTGCCGAAGAGGCGCCCAAGAAGAAGCCCGGCCGCCC13.0303030303030303No Hit
ACATGATACGAGGCCATCAGACGTATATGAGACGACTAGCACGCCATAGC13.0303030303030303No Hit
CTTCAAAAATGGTCATTCAACTTTTTTTTGCTATCTTTTCCTTGCCTCTG13.0303030303030303No Hit
GTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTAC13.0303030303030303No Hit
AGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAGTACTCTGCGTCTGT13.0303030303030303No Hit
GTACATGGGAAGCAGTGGTATTAACGCAGAGTACATGGGAAGCAGTGGTA13.0303030303030303Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCCTTAGAGGGACTATTCCAACATCACTCCTTTGGAATTATTTCAGTCAT13.0303030303030303No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG13.0303030303030303Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTGTGCCAACAGCGACCGCAGCTGCTACGTGTGGAAGGCCTGAAGAACGC13.0303030303030303No Hit
GATTTCACTTCCACTCCATAACGCTCCTCATACTAGGCCTACTAACCAAC13.0303030303030303No Hit
CCACAAAAAGAGTGTTTCAAATCTGCTCTGTGTAAATGAAAGTTCAACTC13.0303030303030303No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGTGTT13.0303030303030303Clontech SMART CDS Primer II A (96% over 26bp)
GGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAA13.0303030303030303No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)