FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33
Sequences flagged as poor quality0
Sequence length140
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTGTCGTCATTGGACACGTAGATTCGGGCAAGTCCACCACTACTGGC39.090909090909092No Hit
AACCTGGAACATCACTGCTTTCTTGAAATGGAACTGACAAGCAATTAACT26.0606060606060606No Hit
TCTTGGTACCCCGGCTCCGGGAGCCCAGCTCCCCGCCACCGCCGCCGCCT26.0606060606060606No Hit
GCTTCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG26.0606060606060606Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTG26.0606060606060606No Hit
GGAGGGGTGCTCTCCTGAGCTACAGAAGGAATGATCTGGTGGTTAAGATA26.0606060606060606No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTAT13.0303030303030303Clontech SMART CDS Primer II A (100% over 26bp)
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT13.0303030303030303No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13.0303030303030303No Hit
AGACTATGGTGAGCTCAGGTGATTGATACTCCTGATGCGAGTAATACGGA13.0303030303030303No Hit
ACGCAGAGTACATGGGGGGAATTCCAATGATAGGCTAGTCTCGCGCAGTA13.0303030303030303No Hit
CTTTCTAAGAGAGTTACTCAACTGGTGATGTAAATCAATCATGGCAGGTG13.0303030303030303No Hit
TGTCCAGGAAATATAACCTTAGGGTTGTGATGGGGGCCAATTATTTATTT13.0303030303030303No Hit
GTACATGGAAGCAGTGGTATCAACACAGAGTACATGGAAGCAGTGGTATC13.0303030303030303Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTC13.0303030303030303No Hit
ACTCTGTGAGTTGAATGTACACAACACAAGGAAGTTACTGGGAATTCTTC13.0303030303030303No Hit
GCCTATTACCATACCAGTGTATGTATTCTTCCACCTTTGGACTTGGATGG13.0303030303030303No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTATA13.0303030303030303No Hit
TGACAAAAGAACAAAACAGATACATTGGACTTCATTAAAATTAAAAACTT13.0303030303030303No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG13.0303030303030303Clontech Universal Primer Mix Long (96% over 26bp)
GGTATATCTGCTCATGGCCTCGAGCTCCATTTTGGAGCTCGAGGCCATGA13.0303030303030303No Hit
CGTTCCTTGGCCTTGCTGATCTTCATGCGCATGGGCTTGGCCTTCTCGAC13.0303030303030303No Hit
CTCCTGAAATGCTGTGATTTACATGTGTGAGCCACCGCACCCAGCCCTCT13.0303030303030303No Hit
GTTATTATCACTGTATTTCTTATGTTCAAAAGGTGGAAGAAAGATGGAGC13.0303030303030303No Hit
CAAGCAGACAATAGAGTGTGTCTACCCATGCAGAAAGTTCTGGTCCACCA13.0303030303030303No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACTCTGT13.0303030303030303No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)