FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45
Sequences flagged as poor quality0
Sequence length140
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT36.666666666666667Clontech Universal Primer Mix Long (96% over 26bp)
GTGTTCGATGTGTCGATAGTGTCCTGCCGGCCCGCGCTCACGAAGCGCAC36.666666666666667No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36.666666666666667Clontech Universal Primer Mix Long (95% over 22bp)
ATAGAAGATCATGTACCCGGCCGTCAGCCGGCTCTGCGCCTCGGGCCGCA24.444444444444445No Hit
CAGTGGTATCAACGCAGAGTACATGGATGTACGGCGACCACCGAGATCTA24.444444444444445Illumina Single End PCR Primer 1 (95% over 22bp)
TCCGCACTGGCTTGCGGTTCGCTCAAGGCCCGCTGCACTTGGTGATCCGA24.444444444444445No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG24.444444444444445Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12.2222222222222223Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGTCGAGCCTGCAGCGGCTGCGGTGGAGCCTGCGCCCCAGCCGCCGGCCC12.2222222222222223No Hit
ATAACAAATAAGATAACAACAGACCAAAAAAAACGTAAACAACACAATAT12.2222222222222223No Hit
GTACATGGGCGCACCACTCTACCTTTTTTCCTACACGACGCTGATCGGAA12.2222222222222223No Hit
CCAGAAGCCAGCCTGCATCAGGGCCCACGGATCGAGCGCGACCACCACGG12.2222222222222223No Hit
GTCTGAAATATCATCTGAAATGGCAGCTTTAAGCTTAGCTTTAATATGAT12.2222222222222223No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT12.2222222222222223Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG12.2222222222222223Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTTAAGCAGTGGTATCAACGCAGAGTACATGGGTCGCCCCGGTTAAAC12.2222222222222223Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT12.2222222222222223Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTTATACACATCTCC12.2222222222222223Clontech Universal Primer Mix Long (95% over 21bp)
GGCCCACCGAGTCTCACTCTTTCCCTACACGACTCTCTTCCGATCTCATG12.2222222222222223Illumina Single End PCR Primer 1 (96% over 32bp)
GGCCTACTTCCTGGTCTCCAAGGGCACGATCGCCGGCGATTCGCTCAAGT12.2222222222222223No Hit
ATCGGACAGTGGCTGGCGCAGGATGATTTCTCCGGTGAGGTGCCGTATCA12.2222222222222223No Hit
GCCCGAGGCCGTGGCGCGCTACCTGCAGCTGCACCTGGCGGCGCGCCAGC12.2222222222222223No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC12.2222222222222223Clontech Universal Primer Mix Long (96% over 26bp)
CTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCTGCAGAACTTGG12.2222222222222223Illumina Single End PCR Primer 1 (100% over 30bp)
CTAGAAGATCGGCAGGCCGAACTTGTCCGCCAGCTCCATCAGCCGCTTGG12.2222222222222223No Hit
GGTATAGGGACACCGAGATATACATCTTTCCCAAAACGACGCTTTCCGAT12.2222222222222223No Hit
ACCTAGAGCAGCCAATCAGTCAGCCCCAGTTCCTGACTGCACCCCGCTGT12.2222222222222223No Hit
GCGCGGCACTGGGCATCGGCTGGCTGCACCACGTCTTCAAGCCGCTGGCA12.2222222222222223No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT12.2222222222222223Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA12.2222222222222223Clontech Universal Primer Mix Long (95% over 21bp)
GAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG12.2222222222222223Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATGACGGCGAC12.2222222222222223Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCAAGAGCGTCGTGTAGGGAAGAGTGTAGACTCGGGGTCGCTACATTAC12.2222222222222223No Hit
GTACCTGCGGTTTACGCGACACGCACCATAGAGCACCGCCGGGCGCGTGT12.2222222222222223No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACACATCTCC12.2222222222222223Clontech Universal Primer Mix Long (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)