FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA511.11111111111111Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCCCGGGCCAAGGTCCTGGCTGAGCTGGAGCCAAAACTCGCCGCTGCGT24.444444444444445No Hit
ACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGTCCC24.444444444444445Illumina Single End PCR Primer 1 (100% over 43bp)
GTCATCGACTTCGGCCTGCAGTCGGTGCACGTGGCCAACCAGAGCCTGAT24.444444444444445No Hit
CACCCGCGACGTGACCGGCGGCTTCACCGAAACCGTGAGCGGCGGCGTGA24.444444444444445No Hit
CATCAGACCTGTGACAACACGCGCCCGGCGGTGCTCTATGGTGCGTGTCG12.2222222222222223No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTCTTACCATGTACT12.2222222222222223Clontech SMART CDS Primer II A (95% over 22bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCCTGATACGGCGACC12.2222222222222223Clontech Universal Primer Mix Long (96% over 26bp)
GTGTAGGGAAAGAGTGTAGATCCGGTGGTCCCATGTACTCTGCGTTGATA12.2222222222222223Illumina Single End PCR Primer 1 (95% over 22bp)
GTACATGGGAAGCAGTGATATCAACGCAGAGTACATGGGAAGCAGTGGTA12.2222222222222223Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTGCAGGGCGCGCAGCACCACCTCCCGGGGGTAGACGCTGGTGCGCGTG12.2222222222222223No Hit
CAGCAGCACGTCGCCTGCCAGCGAAGCGACCAGGCCCGCCACCAGCAGGC12.2222222222222223No Hit
GCCGTTGGACTTGCTGCGCTTGGCGCGGGCCGGCGGCTGGGGCGCAGGCT12.2222222222222223No Hit
ACAGTTGCTGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGGATCAT12.2222222222222223Illumina Single End PCR Primer 1 (97% over 36bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGTACGGCCCACCGAGTCT12.2222222222222223Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG12.2222222222222223Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA12.2222222222222223Clontech Universal Primer Mix Long (96% over 26bp)
CTCCTCAGTCTGCCGGCGCGAGAGCTGGGCGATGTAGGCCCGCTTCACCG12.2222222222222223No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC12.2222222222222223Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC12.2222222222222223Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTTATACACATCTGA12.2222222222222223Clontech Universal Primer Mix Long (95% over 21bp)
CACCCGCGACGTGACCGGCGGCTTCACCGAAACCGTGAGCGGCGGCTTGA12.2222222222222223No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACACATCTGA12.2222222222222223Clontech Universal Primer Mix Long (95% over 21bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG12.2222222222222223Clontech Universal Primer Mix Long (96% over 26bp)
GATCTACACTCTTTCCCTACACGACGCTCATCCGAACTGTCCCAGGTCTT12.2222222222222223Illumina Single End PCR Primer 1 (97% over 35bp)
CAACGCAGAGTCCATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA12.2222222222222223Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTCGTTTTTAACAAATCT12.2222222222222223No Hit
CGGGGTGCAGTCAGGAACTGGGGCTGACTGATTGGCTGCTCTAGGTCTGT12.2222222222222223No Hit
ATTCAAAATCTGGTTCAAACCTCGCTACGTTCTATCGTCGGTGAGATGGA12.2222222222222223No Hit
CTTCCGATCTATCCCTTTTTTTGGGCCTGTTGGTACCAAAAAAGGGATAG12.2222222222222223No Hit
ACGCTGAGTACATGGGAAGCAGTGCTATCAACGCAGAGTACATGTAATGT12.2222222222222223Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ATACCACCACTAATCAGTAAAGGAATTTGCTGATCGCGAGCAATCTTACA12.2222222222222223No Hit
GTGATCGCACCTTCGCCGACGACCTGTCGATCGTGGGCGGTCTGGCGCGC12.2222222222222223No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC12.2222222222222223Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAG12.2222222222222223No Hit
GCGCACCTGATGTCGATCTCGGGCCTGCACGTCACGATGTTCGCCTGGCT12.2222222222222223No Hit
GGGAAAGAGTGAACCGTGTCGGTTCATCCCATGTACTCTGCGTTGATACC12.2222222222222223No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)