FastQCFastQC Report
Tue 10 May 2022
EGAF00003643843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281719
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC15660.555873050805945No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC14330.508662887487177No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG12000.42595636077083904No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA10890.3865553973995364No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA10670.37874619745207105No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT10670.37874619745207105No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC10490.37235685204050845No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT10440.37058203387062993No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC8480.3010091616113929No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC7920.28113119810875375No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT7880.279711343572851No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA7720.27403192542923976No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT7420.2633830164099688No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG7330.26018834370418753No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT7120.25273410739069785No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT6820.24208519837142684No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT6420.22788665301239885No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG6280.2229171621367391No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA6200.22007745306493348No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC6070.21546292582324938No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC6040.2143980349213223No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA5980.21226825311746814No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA5930.21049343494758962No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG5790.2055239440719298No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT5690.20197430773217284No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT5370.19061547144495047No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT5310.18848568964109627No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA5300.18813072600712058No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA5170.18351619876543648No Hit
CTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCT5160.1831612351314608No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG4890.1735772170141169No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG4830.1714474352102627No Hit
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG4690.16647794433460292No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC4690.16647794433460292No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT4580.16257334436087023No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA4490.15937867165508893No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT4440.15760385348521044No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG4420.15689392621725903No Hit
ATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATG4280.15192443534159925No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC4190.14872976263581797No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC4190.14872976263581797No Hit
GAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCG4040.14340530812618246No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT3990.14163048995630398No Hit
GCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGC3970.14092056268835257No Hit
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA3960.14056559905437688No Hit
GTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATT3960.14056559905437688No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG3900.1384358172505227No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC3890.13808085361654698No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG3870.13737092634859557No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT3830.13595107181269278No Hit
TTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGA3820.1355961081787171No Hit
CTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGA3820.1355961081787171No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG3740.1327563991069115No Hit
GAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGG3570.12672201732932462No Hit
ATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAAGGC3560.12636705369534892No Hit
CTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTGGC3520.1249471991594461No Hit
GCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCT3500.12423727189149472No Hit
ATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTG3470.12317238098956762No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG3460.12281741735559193No Hit
ATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGC3410.12104259918571342No Hit
TCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCA3350.11891281738185923No Hit
GTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGT3350.11891281738185923No Hit
TCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGC3340.11855785374788354No Hit
CACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTGGT3320.11784792647993213No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA3300.11713799921198073No Hit
GCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTC3250.11536318104210225No Hit
GAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTAT3210.11394332650619943No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA3200.11358836287222374No Hit
ATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCG3190.11323339923824803No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG3140.11145858106836955No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA3140.11145858106836955No Hit
GGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCC3120.11074865380041815No Hit
ACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGA3090.10968376289849104No Hit
GTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGCCTT3080.10932879926451534No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA3070.10897383563053964No Hit
GAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGG3050.10826390836258826No Hit
GGACAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGG3040.10790894472861255No Hit
CTTTACAGCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATGACG3010.10684405382668546No Hit
GGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA2990.10613412655873407No Hit
CATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTGCCAT2970.10542419929078266No Hit
GGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATA2960.10506923565680695No Hit
TGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACGAAGCAGCCTC2950.10471427202283126No Hit
GGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGG2950.10471427202283126No Hit
GTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCT2940.10435930838885556No Hit
GTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGT2920.10364938112090416No Hit
GATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCGA2910.10329441748692847No Hit
GCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCC2910.10329441748692847No Hit
TTTATGAACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTT2890.10258449021897707No Hit
CTCGTGGAAGAGCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTC2880.10222952658500138No Hit
GCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGA2880.10222952658500138No Hit
ATTCAAATGAGCATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGA2880.10222952658500138No Hit
TCATGCGACTGTTCCCCGGGGTCAGGGCCGCTGGTATTTGCTGTAAAGAG2870.10187456295102566No Hit
GTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAAC2860.10151959931704996No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG2860.10151959931704996No Hit
CTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTC2860.10151959931704996No Hit
ATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCA2830.10045470841512287No Hit
GAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCT2830.10045470841512287No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)