FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003643894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length140
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGCGTCATGAGGACCTACAGTTCGCCAAGGCTTCGGGTGTAGGCAA627.27272727272727No Hit
ACCGAGCAGGGCGACGAGATCGGAGCGGGTCATGGGCGGGTCGAAAAGAA418.181818181818183No Hit
GTAACGCCATAATTTTTAAAGCTACATAAGATTTTCTTCCTTAAGAAGAA29.090909090909092No Hit
CCCGCACGCTGAATCGCCCACGTCTGGGCCGCAGGCACCAGCCCCGAGAC14.545454545454546No Hit
CGGGGGCGTAGCGGTACATGGCGAACACGGGGTGATGGTTGACGGTGTAG14.545454545454546No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14.545454545454546No Hit
CCTCATGACGACCTATGACGGCACTCTTGAGGAGGTTGCCGCCCAGGTCC14.545454545454546No Hit
AAATTAGCCAGGCGTGGTGGTACATGCCTGTAATCCCAGCAGCTCGGGAG14.545454545454546No Hit
GCCCAGCACGTCGGCATACAGCAGTCGGCGCACCGTCCGCATGGCGGGGT14.545454545454546No Hit
CTGCGGCACCGTGATTTCGCCGCGGGCCATGGGCCTGCGCGCGGTGCGCG14.545454545454546No Hit
AGTCGGTGACGGCCACCGGAGTTGCACCAGAGACAGCCACATTCCGGTAA14.545454545454546No Hit
CCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTG14.545454545454546No Hit
CTGCTGTTCGAGGAGCAGGACTTCGACGCCCTGGCCAGGCGCCTGCCGGA14.545454545454546No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)