FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1356
Sequences flagged as poor quality0
Sequence length140
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT886.489675516224189Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA775.678466076696165Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG372.728613569321534Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC352.5811209439528024Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG312.286135693215339Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT292.1386430678466075Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG211.5486725663716814Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG211.5486725663716814Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG201.4749262536873156Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC161.1799410029498525Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC161.1799410029498525Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC141.0324483775811208Clontech Universal Primer Mix Long (96% over 26bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT130.9587020648967551Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC120.8849557522123894Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG120.8849557522123894Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT120.8849557522123894Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA120.8849557522123894Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT110.8112094395280236Clontech Universal Primer Mix Long (100% over 23bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT110.8112094395280236Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA110.8112094395280236Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA110.8112094395280236Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC100.7374631268436578Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT100.7374631268436578Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA100.7374631268436578Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC90.6637168141592921Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC90.6637168141592921Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC80.5899705014749262Clontech Universal Primer Mix Long (96% over 25bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA80.5899705014749262Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC70.5162241887905604Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC70.5162241887905604Clontech Universal Primer Mix Long (96% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT70.5162241887905604Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT70.5162241887905604Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA70.5162241887905604Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGG70.5162241887905604Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG60.4424778761061947Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT60.4424778761061947Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG60.4424778761061947Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA60.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT60.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA60.4424778761061947Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA50.3687315634218289Clontech Universal Primer Mix Long (95% over 21bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG50.3687315634218289Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG50.3687315634218289Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG50.3687315634218289Clontech SMART CDS Primer II A (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG50.3687315634218289Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGATACCACTGCTTCCCATGT50.3687315634218289Clontech Universal Primer Mix Long (95% over 24bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC50.3687315634218289Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG50.3687315634218289Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATAC40.2949852507374631Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC40.2949852507374631Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG40.2949852507374631Clontech SMART CDS Primer II A (96% over 26bp)
CAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGT40.2949852507374631Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA40.2949852507374631Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA40.2949852507374631Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC40.2949852507374631Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.2949852507374631No Hit
GTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATG40.2949852507374631Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT40.2949852507374631Clontech Universal Primer Mix Long (96% over 26bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT40.2949852507374631Clontech Universal Primer Mix Long (96% over 26bp)
GTACTGGGAAGCAGTGGTATCAACGCAGAGTACTGGGAAGCAGTGGTATC40.2949852507374631Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT40.2949852507374631Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA40.2949852507374631Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG30.22123893805309736Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTCTGTCTCTTATAC30.22123893805309736Clontech Universal Primer Mix Long (96% over 26bp)
TCCCAAGTACTCTGCGTTGATACCACTGCTTCCCAGTACCTGTCTCTTAT30.22123893805309736Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA30.22123893805309736Clontech Universal Primer Mix Long (95% over 22bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA30.22123893805309736Clontech Universal Primer Mix Long (96% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA30.22123893805309736Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA30.22123893805309736Clontech Universal Primer Mix Long (96% over 25bp)
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGAT30.22123893805309736Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG30.22123893805309736Clontech SMART CDS Primer II A (96% over 26bp)
TTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT30.22123893805309736Clontech Universal Primer Mix Long (96% over 26bp)
GCGTGGCCCCGTCCTCGGCCTTGGGCGAAGAAGTCGAGGAGGCGGCCGAC30.22123893805309736No Hit
GGATTATTAACCCATTCACCATCGGCAGTAAGCCGCGAGAAACCAGTATC30.22123893805309736No Hit
TGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG30.22123893805309736Clontech Universal Primer Mix Long (96% over 26bp)
CCCAAATGCTGTTGTATCTGACTGAGGACAATGCCCTGTCCTCCGGCGTC30.22123893805309736No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAATACATGGGAAGCAGTGGTA30.22123893805309736Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TTCCAAAGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA30.22123893805309736Clontech SMART CDS Primer II A (100% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTTAT30.22123893805309736Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGATACCACTGCTTCCCATGTAC30.22123893805309736Clontech Universal Primer Mix Long (95% over 21bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC30.22123893805309736Clontech Universal Primer Mix Long (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCCTGTCTCTTATACACAT30.22123893805309736Clontech Universal Primer Mix Long (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.22123893805309736No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA30.22123893805309736Clontech Universal Primer Mix Long (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.22123893805309736No Hit
TCCCAGTACTCTGCGTTGATACCACTGCTTCCCAGTACTCTGCGTTGATA30.22123893805309736Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTACGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.14749262536873156Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT20.14749262536873156Clontech Universal Primer Mix Long (96% over 26bp)
GACCAAGGGTTCCAGTCCAACGTTAATCGGGGCTGGGTGAGTCGACCCCT20.14749262536873156No Hit
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA20.14749262536873156Clontech Universal Primer Mix Long (95% over 22bp)
ACTCAGACCGCTGTGGCCCGTGAGCTCGGGGTGTCCCAGCCGAGCGTTGC20.14749262536873156No Hit
CTGCTTCCCCTGTACTCTGCGTTGATACCACTGCTTCCCCTGTACTCTGC20.14749262536873156Clontech Universal Primer Mix Long (96% over 28bp)
ACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGC20.14749262536873156Clontech SMART CDS Primer II A (100% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGACTCTGCGTTGATACC20.14749262536873156Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTG20.14749262536873156Clontech Universal Primer Mix Long (96% over 26bp)
TACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT20.14749262536873156Clontech Universal Primer Mix Long (96% over 26bp)
CTGGTTGATACCACTGCTTCCCATGTACTCTGGTTGATACCACTGCTTCC20.14749262536873156No Hit
CCCAAGTACTCTGCGTTGATACCACTGCATGGGAAGCAGTGGTATCAACG20.14749262536873156Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTCTGT20.14749262536873156Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTCCCCATG20.14749262536873156Clontech SMART CDS Primer II A (96% over 25bp)
GACGTGACAATTGCGGGAGGAGCATGAATCAGCTGTTCCTTCGGGAGGAG20.14749262536873156No Hit
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT20.14749262536873156Clontech Universal Primer Mix Long (96% over 26bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA20.14749262536873156Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT20.14749262536873156Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAG20.14749262536873156Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCATCCCATGTACTCTGCGTTGATACCAC20.14749262536873156Clontech SMART CDS Primer II A (95% over 21bp)
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAATTA20.14749262536873156Clontech Universal Primer Mix Long (95% over 21bp)
GTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTAC20.14749262536873156Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTC20.14749262536873156No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACACGGGAAGCAGTGGTA20.14749262536873156Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACCACTGCTTCCCATG20.14749262536873156Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTT20.14749262536873156Clontech SMART CDS Primer II A (96% over 26bp)
CAGCTGAGCTGCCCTTGTTCCTGCTCTGGTCTTTCACCTCCTCGATATCT20.14749262536873156No Hit
TATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGG20.14749262536873156Clontech SMART CDS Primer II A (100% over 26bp)
GATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC20.14749262536873156Clontech SMART CDS Primer II A (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.14749262536873156Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGT20.14749262536873156Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA20.14749262536873156Clontech Universal Primer Mix Long (95% over 21bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT20.14749262536873156Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT20.14749262536873156Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGAGTACTGGGAAGCAGTGGTATCAACGCAGAGTACTTT20.14749262536873156Clontech SMART CDS Primer II A (100% over 26bp)
GAGTACATGGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGG20.14749262536873156Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT20.14749262536873156Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACAGGGGAAGCAGTGGTATCAACGCAGAGTACAGGGGAAGCAG20.14749262536873156Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTT20.14749262536873156Clontech Universal Primer Mix Long (96% over 26bp)
TATCTTCTGTAGCACTACGAAGTCGTCGATGACGTCTAACTCGATCACCG20.14749262536873156No Hit
TCCCAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.14749262536873156Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTT20.14749262536873156Clontech Universal Primer Mix Long (96% over 26bp)
AACCGAGGCCACCCGGAGCCGTGCCCAGTCCACGCCGGCCGTGCCCGGCG20.14749262536873156No Hit
TACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT20.14749262536873156Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT20.14749262536873156Clontech Universal Primer Mix Long (96% over 26bp)
GGAGGAAGCTATGTCTCATACAAACTGCTGACCTCAATTGATTACTTACA20.14749262536873156No Hit
TATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGG20.14749262536873156Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGT20.14749262536873156Clontech Universal Primer Mix Long (96% over 26bp)
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT20.14749262536873156Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTCCATGTACCCTGCGTTGATACCACTGCTTCCATG20.14749262536873156Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCTGTACTCTGCGTTGATACC20.14749262536873156Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACG20.14749262536873156Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGATATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14749262536873156No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT20.14749262536873156Clontech Universal Primer Mix Long (96% over 25bp)
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA20.14749262536873156Clontech Universal Primer Mix Long (95% over 21bp)
GAGTACTGGGAAGCAGTGGTATCAACGCAGAGTACTGGGAAGCAGTGGTA20.14749262536873156Clontech SMART CDS Primer II A (100% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT20.14749262536873156Clontech SMART CDS Primer II A (96% over 26bp)
GTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCA20.14749262536873156No Hit
CCATGTACTCTGCGTTGATACCACTGCTCCCATGTACTCTGCGTTGATAC20.14749262536873156Clontech SMART CDS Primer II A (96% over 25bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA20.14749262536873156Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCATGGTCGAGACCATGCGGCGCGAAGGACATAGCGCCCTGGTGCACA20.14749262536873156No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT20.14749262536873156Clontech SMART CDS Primer II A (100% over 26bp)
ATGGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA20.14749262536873156Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AATGAGATGAGACTGCAAATTTTGTTGTTAGGCAGTCACCATTTATGACC20.14749262536873156No Hit
TTTTTGGGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGATACCACTG20.14749262536873156Clontech SMART CDS Primer II A (100% over 23bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.14749262536873156No Hit
ATACCACTGCTTCCCAGTACTCTGCGTTGATACCACTGCTTCCCAGTACT20.14749262536873156Clontech Universal Primer Mix Long (96% over 26bp)
CTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG20.14749262536873156Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)