FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003643943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACATAACATTAAAACTGAAGGGGAAAAAAAACCAAAAACCAGTTTGTT211.11111111111111No Hit
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT211.11111111111111Clontech Universal Primer Mix Long (96% over 26bp)
ATCTGGGTCATCTTCTCGCGGTTGGCCTTGGGGTTCAGGGGGGCCTCGGT15.555555555555555No Hit
TTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATATTTCGTCACT15.555555555555555No Hit
AAACAGATAGGGGGATTATATGGGTATGTGTCCAGTAGCCATAGGCATAT15.555555555555555No Hit
CGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATC15.555555555555555No Hit
GGCACAGTGTGGGTGACCCCGTCACCGGAGTCCATCACGATGCCAGTGGT15.555555555555555No Hit
GTGCCCAGTGGACAGGTTTCTGACCAGAAGAAGGAATGCCCATTAACAAC15.555555555555555No Hit
CTTCGCAGGAACAGGGGTAGGTGACCTCAGGGCGATTCATGAGCTCAGCG15.555555555555555No Hit
ATCTCACACTGTCTCCCACCCACCTCCTCCACTTAGTGGCCTTTTCTCCT15.555555555555555No Hit
CTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAGTAATT15.555555555555555No Hit
GAGGGTGGGGGTGGAGGTCCTGCTCCTAGAGATGAACTCTATCCAGCCCC15.555555555555555No Hit
TCCTTGCACCTCTGGGCCACATCCTGGGCAGCCAACATAGCAGCATATGG15.555555555555555No Hit
GACGACAGCCTGTGTCGCATCTGCATGGATGCCGTCATCGACTGTGTCCT15.555555555555555No Hit
GCTGTAAAGACAAGCAGGAGGGTCGACATGGACTGAAATCACCCTCTGCA15.555555555555555No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG15.555555555555555No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)