FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003643944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG211.11111111111111Clontech Universal Primer Mix Long (96% over 26bp)
GGTCTGTTCGACCAGACATATCCTAGCTAAGGGATGTCCAAACATCAGAA15.555555555555555No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGATGCGTGC15.555555555555555No Hit
CTTCAACAACTTCCCTATCTTTAATCCTCTCACTCCACAAATTCATAAGC15.555555555555555No Hit
GGTCAGAAGGATTACTATGTGGGCGACGAGGCCCAGAGCAAGAGAGGCAT15.555555555555555No Hit
GTCTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAA15.555555555555555No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC15.555555555555555No Hit
AATACATGAGGTGCATTATTAAGGTTTGACAACATGGTGACTGCCTATAA15.555555555555555No Hit
TCATGGCGGTGTCGGAGAGCCAGCTCAAGAAAATGGTGTCCAAGTACAAA15.555555555555555No Hit
GGGCTGCATCGGCGCCGTCAACGAGGTCCGCTGCCTGCTGGGGCTGTACT15.555555555555555No Hit
GGTCAGTTCGACCAGACATATCCTAGCTAAGGGATGTCCAAACATCAGAA15.555555555555555No Hit
GTACATGGGGCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGC15.555555555555555No Hit
CTCATCTACATGTCTACTTTGTTACTAGCCTTTCGAGTAGTGCAGGGCCA15.555555555555555No Hit
GTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTTCCCCT15.555555555555555No Hit
TTCCTGCAGTCCAGCCTGAGGGCTCTTCGGCAAATGTAGCATGGGCACCT15.555555555555555No Hit
CCTTCACGGTTTGCTGGTGTGTAGCCTCCTGGATACAGGCAATGTATATA15.555555555555555No Hit
ACTTGTGGACACTGATCTCATCCAAAGAGGACAGAAGTTTCAATCATCTG15.555555555555555No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)