FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143
Sequences flagged as poor quality0
Sequence length140
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA322.799650043744532Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT151.3123359580052494Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG110.9623797025371827Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC90.7874015748031495Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT80.699912510936133Clontech Universal Primer Mix Long (100% over 23bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG70.6124234470691163Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG70.6124234470691163Clontech Universal Primer Mix Long (96% over 26bp)
GTGCAAGCGTGACTTGAAGTGTTGCATGGGCATGTGTGGGAAATCCTGCG50.4374453193350831No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA50.4374453193350831Clontech Universal Primer Mix Long (95% over 21bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG50.4374453193350831Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ATAAAATTCATGAGCCAAAAAACCCAAAACAAAACTAAAAACAGGGAAAA40.3499562554680665No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC40.3499562554680665Clontech Universal Primer Mix Long (96% over 26bp)
GAATAAGCTTTTGTTCTCTTTTCTGTTTTGCAAGAACTTCATCATATAAT30.26246719160104987No Hit
GTGTTCAGATATGTTAAAGCCACTTTCGTAGTCTATTTTGTGTCAACTGG30.26246719160104987No Hit
CCCACACTTAGAAAAAATAAATAGTTAATAAATTATAGGCAAACCAGTTG30.26246719160104987No Hit
GTGCAGAATTCATAGTGTAACAAGAACATATAAGGGAAGAAGGAGAGGTC30.26246719160104987No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC30.26246719160104987Clontech SMART CDS Primer II A (96% over 26bp)
CTAATGACCTGTATCTATTAAATGTCTGTGATGTACCAAGAATAAAGCAG30.26246719160104987No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG30.26246719160104987Clontech Universal Primer Mix Long (96% over 26bp)
GGATTGTGGGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTT30.26246719160104987No Hit
AGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGG30.26246719160104987No Hit
ATCAACGCAGAGTACATGGGAGAGGAACGGGCCATTTGTGAGTACAATGA30.26246719160104987No Hit
GAAGTCTTCCTGAGGACTATGGACCAAGGTCTAGAGCCTGGGAGCAGTTT30.26246719160104987No Hit
GTGTTGAGAGGCACTGACAAGATTGAATTCTAATTCATAAACAGGATTAT30.26246719160104987No Hit
GCCGATCCCACGCAAAGAGCAGAACGCCATTGCTGAGCGCTTTATCCGCC30.26246719160104987No Hit
GATGCTGGGCCTCTACTGGAGGCCTAGTTCCGACTGTGTTGCCCTCTCCT30.26246719160104987No Hit
CACACACACAGATGCACACATACACTTACAAATATTTTTCTTCCCTCCAT30.26246719160104987No Hit
GTATATTGAAGAGATAATCTGCATGCCCATGTTTATTGTTATTACAACAG30.26246719160104987No Hit
GACCTGCACGAGGCCGGCTGCGACATCATCACCATCACGCAGTACGTGCG30.26246719160104987No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA30.26246719160104987Clontech Universal Primer Mix Long (96% over 26bp)
GGATATTGCTGAATGCAGCTGGGAAAGGTTCTTTGGCAAAGACTAATCAG30.26246719160104987No Hit
GGCTACGGTTTATTCAAATCAAACATCAGCTGTTTGCTTGGCGAGCTCTA30.26246719160104987No Hit
GTCTTGATTACTGGCCAGCGTCACACTCTGCCTAAAAGCGGCTTGAGTGT30.26246719160104987No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG30.26246719160104987No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.26246719160104987No Hit
CTCCTCCACTGGGCAGACAGGCCCTCAGAGTGCTCTGTAGGAGCTGTAGA30.26246719160104987No Hit
CTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTT20.17497812773403326No Hit
AAACATAGGCAGGGGCCAGAGCATGGAAGGGGGTTAGTAATTGGAACCTG20.17497812773403326No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCTGTCTCT20.17497812773403326Clontech SMART CDS Primer II A (96% over 26bp)
ATCTAGATCATCAGAAGCATCTTTTTTCCTAGTGTCCTCTTCATCAGCTT20.17497812773403326No Hit
CGCGTGCCCCGCCGCGCGCCGGGACCGGGGTCCGGTGCGGAGTGCCCTTC20.17497812773403326No Hit
CCACCTTCCAGGTTCACGCCATTCTCCCACCTCAGCCTCCGGAGTAGTTA20.17497812773403326No Hit
GGATTTAATTGTAGTATGTTTTTCTCCAACATTCCTGGTACTGTTCCCTT20.17497812773403326No Hit
GATTAATAGTAACTTCTTTAGTTACTATTAATCCAGACACTACTTGTGGC20.17497812773403326No Hit
CTACGAGGCGGGCGATGGGGGAGACCATCTCGTTGATGGAGGCCGAGACG20.17497812773403326No Hit
CTTTGTGGAATTCGAAGATCCTAGAGATGCAGAAGATGCAGTACGAGGAC20.17497812773403326No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20.17497812773403326Clontech SMART CDS Primer II A (100% over 24bp)
ACGCAGAGTACATGGAAGCAGTGGTATCAACGAAAAAAGTACTCTGCGTT20.17497812773403326No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC20.17497812773403326No Hit
CTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGAGCGTCGTGTAGGA20.17497812773403326No Hit
CGGGGGCAAGGACGCGCACGGGCGTCAGACGACCAGATGCGCGGCGGCGT20.17497812773403326No Hit
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT20.17497812773403326No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT20.17497812773403326Clontech SMART CDS Primer II A (100% over 26bp)
GACCTGGACCACACAGAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGA20.17497812773403326No Hit
TCTTTATAGCAGCATGATTTATAGTCCTTTGGGTCCCATGTACTCTGCGT20.17497812773403326No Hit
GTCTTGAACTCCTGACCTCAGGTGATCCACCCACTTTGGCCTCCCAAAGT20.17497812773403326No Hit
AAAGAAACTTTTAAATTCTAATAAAAGTGGGGCAGCCTTCAACCAGAGCA20.17497812773403326No Hit
AGACAGGGTTTCACCATGTTGGACAGGATGGTCTTGATCTCCTGACCTCG20.17497812773403326No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT20.17497812773403326Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA20.17497812773403326No Hit
GGTCTTGAACTCCTGACCTCAAGTGATCTGCTCACCTCGGCCTCTCAAAG20.17497812773403326No Hit
AAACAACAATCTAACAAAAAACGAATATATACCAATCTATCACATATACA20.17497812773403326No Hit
AATGAAAGCTAAGCCTCGGGCTAATTTCCCCATAGCCGTGGGGTGACTTC20.17497812773403326No Hit
GGCGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTGGTGG20.17497812773403326No Hit
CTGTCACCCAGGCTGGAGTGCAGTGGCACGATCTCGGCTCACTGCGACCT20.17497812773403326No Hit
GTAACAAAACATACATTAACAAGAAATAATCTCTCTTACATGATTCAGGC20.17497812773403326No Hit
GGTGTGGATTGGATGCTGGGGTGTGGGGGTTGGAAGCTGTCTGTGGCCCA20.17497812773403326No Hit
TTTTAAGTCAGTTTGGTACATGATACAGGCAGTATCTGCCTGTGCAACAA20.17497812773403326No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTG20.17497812773403326No Hit
GTTGTCCTATATTTATAAGCGTTGAACCAATATGGATACTAACTGAAGTC20.17497812773403326No Hit
GCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA20.17497812773403326No Hit
GAAACTTCTAACTAGTTGTACAACTAATCCGTGACAAATTACCAGATTAA20.17497812773403326No Hit
ATTTGTAGGTGCCTTTTTTTTTCCTGTAGAGATGAGGGTCTTGCCATGTT20.17497812773403326No Hit
CTGTGGTCCCAGCACTTTGGGAGGCTGAGACGGGAGGATCAGTTGAGCCC20.17497812773403326No Hit
ATTTACCGCTCAGTAAGCTATTTTTTGCTCCATATCATCAAGTTTCCAGA20.17497812773403326No Hit
ATTATAGGGAGTTCCTGTTATGGTGCAACAGGCTATGAACCCAACTAGTA20.17497812773403326No Hit
GGCTCATTCAGAGAAGCTCACTTCCACATCAGAGCACTTCCTGGCTCCCT20.17497812773403326No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA20.17497812773403326Clontech SMART CDS Primer II A (96% over 26bp)
CACAATTTTTACGTTGCTGCCAGAAGTCTTCTCAGCATTTGCTTTGAATT20.17497812773403326No Hit
AGCCAACATGATGAAACCCCGTTTCTACTAAAAATACACACAAAAATTAG20.17497812773403326No Hit
ACCGAGATCTACATCTTTCCCCAGGTCTTGTCGATACCAGAACAGATCGG20.17497812773403326No Hit
TTACAGGTGTGAGTCACCACACGTGGCCTTGCACCTAGTTTTAATAAGTA20.17497812773403326No Hit
GTATCCCTGCCCTGAGGGCCTCACAGCCAGATCTCCTGAGAGCAGAGTGA20.17497812773403326No Hit
TTCTGAAGCTAACAGGCCACTGCCTTGGCAACAGTAGAAAAAAATATGAG20.17497812773403326No Hit
TGAGAGAACTTGTTAAAAAGGGTTTCAATCCACCTGTTCTTGTTTTTGTT20.17497812773403326No Hit
GGGTGGGAGAATGTTTACAGAAATAGGGGTGGAGAAAAGATAGGAAATCA20.17497812773403326No Hit
AGGAATAACAACATGATTTACGTTAAAATAGATTGAGATGAAGGACATAG20.17497812773403326No Hit
ATGCAGTTCACATAAATTAATTAGTTGGCACTTTGAGTCAAAGATGGGGT20.17497812773403326No Hit
AAATTAGCCAAGCATGGTGGCGGGCACCTGTAATCCCAGCTACTCGGGAG20.17497812773403326No Hit
GGTTAGAGGCCAGGTGCCATGGCTCATGCCTGTAATCCCAGCACTTTGGG20.17497812773403326No Hit
TTTATTTTGTTTTCCTCTGTCCATTGGGGCTGAGAACTGGCCTGCAAAAT20.17497812773403326No Hit
ACACCTTGACCTAACGTCTTTACGTAGGTACTTGCGCTTACTTTGTAGCC20.17497812773403326No Hit
CACTTGAGCCCCAGGGGTTTGAGACTAGCCCGGGCAACACAGGGAGACCC20.17497812773403326No Hit
GCACAGCACCATGCTCAGGATCATCTCGAAGATCATGATCACAGCGACCA20.17497812773403326No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT20.17497812773403326Clontech Universal Primer Mix Long (96% over 26bp)
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCG20.17497812773403326No Hit
GAGGATTACTTGAGCCCAGCAGTTCAAGGTTACAGTGAGCTAATTAGCTG20.17497812773403326No Hit
GTATAAGAGACAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTA20.17497812773403326No Hit
CTTTCTTTTAAGCAAGGAAAATGCAAGTTGATATATTTTACCTGTGCTCA20.17497812773403326No Hit
GAAGCGTCAGATGTGTATAAGAGACAGGAGGTGCCGGTGGAGCCCGAGTC20.17497812773403326No Hit
CTGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGTGGATCACGAGGTCAG20.17497812773403326No Hit
TTTTAATAGAGATGGGGTTTTGCCATGTTGGCTAGGCTGGTCTTGAACTT20.17497812773403326No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA20.17497812773403326No Hit
GCCTACGTCACATAGGGGGTTCTTTTCATTTTCATGATGCTTAATATAAT20.17497812773403326No Hit
CAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGT20.17497812773403326Clontech Universal Primer Mix Long (96% over 26bp)
GGTTAGAATGTGGGACAACCCACCCATAGCTGACTTAACATGTATCAGGA20.17497812773403326No Hit
TGCCAGATTTTCGCCGCAAGTCAGGGCCATAGCGGGGGGCATAACAAGGC20.17497812773403326No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG20.17497812773403326Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC20.17497812773403326Clontech Universal Primer Mix Long (96% over 26bp)
GCACTTTGGGAGGCCGAGGTGGGTGAATCACCAGAGGTCAGGAGTTCCAG20.17497812773403326No Hit
GTCTTGGATACACAGACCGTATTCTTCATCCTAAATTTATTGTGGGCTTC20.17497812773403326No Hit
GGCCTCTTCTTTGGCAAAGAGTCTTTTGATGGCACTTCAACACCCTGAGC20.17497812773403326No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCT20.17497812773403326Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT20.17497812773403326Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGAGCTGAGATGGCGCCACTGCACTCCAGCCTGGGCGACAAAAGCAAGA20.17497812773403326No Hit
GTATTTTTATTGGAGACAGGGTTTCACCATGTTGGGCAGGCTGGTCTTGA20.17497812773403326No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGA20.17497812773403326Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAAAAAAAAAAAA20.17497812773403326No Hit
CCACCGAGTCTACACTCTTTCCCTACACGACGGTCCTGAAAAACGAGCGT20.17497812773403326Illumina Single End PCR Primer 1 (96% over 27bp)
GCGTCCTGTATACGCCCAAAGATCTCCTTGGCATCGTGGTAAAACTTGTG20.17497812773403326No Hit
CTGCATTCCAGCCTGGGCGACAGAGCAAGACTCTGTCTCAAAAACAAAAA20.17497812773403326No Hit
CATTATTTCATAGAAAAAGGAATATAGCAAACGGTCAGGGTCAGGGTTGT20.17497812773403326No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC20.17497812773403326No Hit
GATTATAGCCCTGAACCACTGTGCCGCCACTGCACTCTCTGTCTCTTATA20.17497812773403326No Hit
ATTCGGGAGGCTGAGTCAGGAGAATTGCTTGAACCTGGGAGGCAGAGGTT20.17497812773403326No Hit
ATACACGTACGCACACACACGCACGAACACACATGAACACCCGTGAGCCT20.17497812773403326No Hit
GTCTTGCACTGAGTATATCATGAGGATCCGGTGGCACAGTGCTGTCAAGA20.17497812773403326No Hit
AAATAGAAACAGGGTCTCACTTTGTTACCCAGGCTGGTCTCAAACTCCTG20.17497812773403326No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT20.17497812773403326Clontech Universal Primer Mix Long (95% over 21bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA20.17497812773403326Clontech Universal Primer Mix Long (96% over 26bp)
GGGTCTTGCCATGTTGTCCAGGCTGGTCTTGAACTCCTGACCTCACACAA20.17497812773403326No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA20.17497812773403326Clontech Universal Primer Mix Long (95% over 22bp)
GGCTGAAGCGGCGCGGTCGCCGCGACGACGTGCAGATCGCGACCAAGGTG20.17497812773403326No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.17497812773403326No Hit
TTATTAACATGTCTTTTCTGGACTAAATATCCCACAAATTACCTCAATGA20.17497812773403326No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC20.17497812773403326Clontech SMART CDS Primer II A (96% over 26bp)
GGTAAATATGTAGCCTAATTATAAAATCACACGCAGACACACAGTGAAAA20.17497812773403326No Hit
AACGCACTCACAGTCGCATCATAATCCTCTCTCAAGGACTTCAAACTCTA20.17497812773403326No Hit
CAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA20.17497812773403326No Hit
ACATGGGTGTACGCGCCACCGAGATTACACTCTTCCCTACACCTGTCTCT20.17497812773403326No Hit
GGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCC20.17497812773403326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.17497812773403326No Hit
CAGCAAAGCATTCAAGAGTTGACAGAGCATAAAAATTGGGAAAATTTGCA20.17497812773403326No Hit
ACAACAAATGGCTACCCAAATGGAGGTAGAAAAAAAAAATCAGACTCACT20.17497812773403326No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)