FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA41.4035087719298245Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG31.0526315789473684Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTAAATAACCAAATTATAAAAAAGCTTAAATTTTAGTGTAAGCTGATG31.0526315789473684No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT31.0526315789473684Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC31.0526315789473684Clontech Universal Primer Mix Long (96% over 26bp)
GGATGTACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT31.0526315789473684Illumina Single End PCR Primer 1 (97% over 46bp)
CATTGGGTTTTCTGGCCAGACTCTGTGTATCATGATTGCAGGAGCCTGGG31.0526315789473684No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC31.0526315789473684Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTTATCTATAGTTGATGACGATAGCAAGTTTCATCCTTTAGT31.0526315789473684No Hit
TCATCGAGAAGTCGGGCGGCAAGAACGTGCTGCTCGGCCTCGTGTCCGAG20.7017543859649122No Hit
GCTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCG20.7017543859649122No Hit
TGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG20.7017543859649122Clontech Universal Primer Mix Long (96% over 26bp)
CGTTTTTCAGGACCTGTTGGTACCAGATCGGAAGAGCGTCGTGTAGGGAA20.7017543859649122Illumina Single End PCR Primer 1 (100% over 26bp)
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAGTATTTTTTTTT20.7017543859649122No Hit
GTACATGGGTGATACCGCCACCGAGATCTACACTCTTTCCCTACACGACG20.7017543859649122Illumina Single End PCR Primer 1 (97% over 34bp)
GGCGTGGCTTGTGTGTTCGGTTTCATGGGGGTCCCTTTTTGGGCCTGTTG20.7017543859649122No Hit
GGCCGAGCGCACCGTGGCGTGGATCGCGGAGCGGCACGGCCCCGAGGTCG20.7017543859649122No Hit
CCTGGAAACATGGCGCACAGCAGCGCGCCGAGATCATGCCTGGCCGGCGG20.7017543859649122No Hit
CGCCATACGGGATGAACTGCAACGGTTCGCTGGCGATGCTCACCGCCTTC20.7017543859649122No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA20.7017543859649122Clontech Universal Primer Mix Long (96% over 26bp)
AGTGGTATCAACGCAGAGTACATGGGAACGCACCGAGATCACACTTTCCC20.7017543859649122Clontech SMARTer II A Oligonucleotide (100% over 21bp)
GATCGGAAGAGGTCGTGTAGAAGTAGGTGCCCATGTACTCTGCGTTGATC20.7017543859649122No Hit
GCTCACCAGGAATTTCCCTGTGGGCAAAATATAGGGAAGGCAGGTATTTT20.7017543859649122No Hit
GTTCAGCCCGGCTGAAGCTGACCGAATGAGACTATGGGCTGTGCCTCCGC20.7017543859649122No Hit
CAGCGGGGCCCTGTTGCCGCGCAGCCGTCTGCCTGCCTCGCGTGACCTGG20.7017543859649122No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC20.7017543859649122Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA20.7017543859649122Clontech Universal Primer Mix Long (95% over 21bp)
GTATCATTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT20.7017543859649122Clontech Universal Primer Mix Long (96% over 26bp)
CTCTCCCGGGTCTTGTCGATACCAGTACAGATCGGAAGAGCGTCGTGTAG20.7017543859649122Illumina Single End PCR Primer 1 (100% over 22bp)
ATTCTGGAGACATTCCAATGTATGTTGCCTTTAAAAAGTCACAATGAGAG20.7017543859649122No Hit
GTGTTATAATATTCTGTTTGTCTATATATTTAACTATTACCAGTGAGGTT20.7017543859649122No Hit
CATCTGGACCGCCAGGCTGCTGCCCTAACTCGAAATGGCGGCACCTTCGA20.7017543859649122No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC20.7017543859649122Clontech Universal Primer Mix Long (96% over 25bp)
CCTGACTAATGGCCTCTTATCTGCCTAGAACACTTTCCCAAAAATTCTTT20.7017543859649122No Hit
AATGATCCGCTTCAGCCTCCCAAGGTGCTGAGATTACAGGTGTGAGCCCC20.7017543859649122No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC20.7017543859649122Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCCAAAAAAAAAAAAAAAAAAAA20.7017543859649122Clontech Universal Primer Mix Long (95% over 21bp)
CATTCAATTACATTTGATGATGATCCCATTCAATTTCATTTGATGATGAT20.7017543859649122No Hit
TCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGA10.3508771929824561No Hit
GAGATGAGTTGTCATGTCCTGCAGCCACTGGTTCTGTGCCTGCAGCTTCG10.3508771929824561No Hit
GCGTTGATACCACTGCCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT10.3508771929824561No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAATGATACGGCTACCACCGAGT10.3508771929824561Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAAAAGAAACTACCATTGGAGTGAACAGACAACCTACAGAATGGGAGAA10.3508771929824561No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAGGG10.3508771929824561No Hit
GTATATATATGATTGCCTGGCTTGTATAATAAGAAAGACCACTGATGTGT10.3508771929824561No Hit
CCTGGATTCAAGTGATTCTTCTGCCCCAGCCTCCCAAGCAGTTGGGACTA10.3508771929824561No Hit
CTAATGTGGAGTGCGACTTGGCCAAAAGAAGTAAGACAGCTTGCTGAAGA10.3508771929824561No Hit
CTTCATAGAAAACATTTGAAATTAATTTAAGGTTTTCAGGGTAAATTATA10.3508771929824561No Hit
CCCACCAGTTCGCCGGCTTCGACCACGAGGCGCTGGCCGACGACCTCGAC10.3508771929824561No Hit
CCACCCTGCTGACCATCGCGGGTGGCCTCGACGAGCCGACGGCAGGCACG10.3508771929824561No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGACGACATCA10.3508771929824561Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTTT10.3508771929824561No Hit
GTAGGAAAGAGTGAGATCTCGGTGCGTATCCTGTCTCTTATACACATCTG10.3508771929824561No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAACTGCGTTTATACCAATG10.3508771929824561No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGTCGGCGACACCGAGA10.3508771929824561Clontech Universal Primer Mix Long (96% over 26bp)
TCAACGCAGAGTACATGGGTGATCGGCGCCACCGCTGTCTCTTATACACA10.3508771929824561No Hit
GAGTACATGGGTGATACCACGGCTTCCCCTGTACACTGCGTTGATACCAC10.3508771929824561No Hit
TCATCTTTGAACTTCTCAAATTTTATGGTAGATGTTACCTATTCCTTCTA10.3508771929824561No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATTTAAGG10.3508771929824561No Hit
ACTGAAATGTGCCATCAAATGAGAGCTGTGAAATAAACTTAATACAAACC10.3508771929824561No Hit
TGATTTGGCTCTTAATTCAAATAAATCAACTTTAAAAAGTTATTTTTGAA10.3508771929824561No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAA10.3508771929824561Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCCAGACTGTGGCTCAGCCCAATTAATGAAAGTAGATGCACACATTGGCT10.3508771929824561No Hit
AGGCTGAGGCGGGCAAATCACCTGAGGTCAGGAGTTCAAGACCAGCCTGG10.3508771929824561No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTAAAACAATACTACC10.3508771929824561No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.3508771929824561No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGTAT10.3508771929824561Clontech Universal Primer Mix Long (96% over 26bp)
ACCCGATCAGCTATCTCTTCGATCGTCACGGCTATCCCGCGCGGACGCCC10.3508771929824561No Hit
ATTCTGACACTGGGACTGGATGAAAAGGACCAGCAAAATACATCTCGCCT10.3508771929824561No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG10.3508771929824561Clontech Universal Primer Mix Long (96% over 26bp)
GGTCGAGGCGCTCGTGCAGCTCTATCGCGACGAGCGCGACCGCGAGGGCT10.3508771929824561No Hit
GAGTTTACTTGGCATCTTAGTCTTATTAAGATGGAAATGAGAAATCTCAA10.3508771929824561No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTCGT10.3508771929824561No Hit
GAGAAGGAGGGAGGCCTGCCTCGGCAGGTGGGCAACAAGACCGAGTGTGC10.3508771929824561No Hit
TCTGAAGAGTGGATGCCCTTGTTATCCCACTTTTCAGATGAAGAAACCAA10.3508771929824561No Hit
GCTCTTCCGATCTGGTACCAACAGGTCCTGAAAAACGAGCGTCGTGTAGG10.3508771929824561No Hit
CCCATGCTCCCGTCGGTCGCCGCGAGCACCGAGGGCTTCGTCCTCGCCGA10.3508771929824561No Hit
ATATACAATTATTACTTGTCAATTATAAAAAAAAGCCCTACTGCAACCAC10.3508771929824561No Hit
CTTTGAGTTACATTGATGGGTGGGCTCACTGATACACACCACACAACTCT10.3508771929824561No Hit
GATGCTCACCTTTCCTCTGAGTCCTCCAGCTGCTCATTTGGAGAGCTGGA10.3508771929824561No Hit
CAATTGCTCGAGATGAACGGCCAGCCGGCCGGCTATGTGCTTTCCCATCC10.3508771929824561No Hit
GGGCGGTGGCTCGCCTCGCGGCGGACCGCCCGCCCGCTCCCAAGATCCAA10.3508771929824561No Hit
ACGCAGAGTACATGGGATGATACGGCGACCACCGAGATCTACACTCTTTC10.3508771929824561Illumina Single End PCR Primer 1 (97% over 35bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCT10.3508771929824561Clontech SMART CDS Primer II A (96% over 25bp)
ATATATATGCAGCAAATATCTTTTACTCCTTCCGTTTTTTTCTACTGAGT10.3508771929824561No Hit
GCTTCCAACCAACAGCCAGACACAACTAGTGTCTTCAAGTCTATAGCACC10.3508771929824561No Hit
NTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT10.3508771929824561Clontech SMART CDS Primer II A (100% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACAT10.3508771929824561Clontech SMART CDS Primer II A (96% over 25bp)
CCCAAGTAGCTGGGATTACAGGTGCCCACCACCATGCCCGGCTAATTTTT10.3508771929824561No Hit
CTTCCTGTCCAAGTTCTGCAGGTACCGGAAGAGCGTCGTGTAGGGAAAGA10.3508771929824561Illumina Single End PCR Primer 1 (96% over 27bp)
TCACCGCGGTTGGGGTCGCCACGACCGTCGGTGTTGTTCGTCAGTATCCA10.3508771929824561No Hit
TGAGCACCACCCCCACCCCACCGGACCCGGCGGGCACACGCCGCGTCGCG10.3508771929824561No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCAGTGGT10.3508771929824561Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTGGGAACATGGCGCACAGCAGCGCGCCGAGATCATGCCTGGCCGGCGG10.3508771929824561No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.3508771929824561Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGTGTACGCGACCACCGAGATCTACACTCTTTCCTACAGCAAC10.3508771929824561Illumina Single End PCR Primer 1 (96% over 27bp)
CTCTTCCAGTGTGGGGGCCACAAGGTCTCTGAACCTGTCTCTTATACACA10.3508771929824561No Hit
GTAGAAGGTCTGAGGAAGATATAATTTCATTTGTATCACTTCAGAAGCAA10.3508771929824561No Hit
GCCGCACGCCGATCCTGATCGACTTCGGGCTCGCCCGCGTGGCCGACGAC10.3508771929824561No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA10.3508771929824561Clontech Universal Primer Mix Long (95% over 22bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.3508771929824561Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC10.3508771929824561Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTGGG10.3508771929824561No Hit
TTAATCGTCTCAACATCAGTGGTTTCAGGATAGCCTGAAGTTTCTGTACC10.3508771929824561No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.3508771929824561Clontech SMART CDS Primer II A (100% over 23bp)
AGGTAGAGGCTCTTACTATCCCCATGTACAGTTGAGGAAAGAAAAGCTTA10.3508771929824561No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACCTGTCTCTT10.3508771929824561No Hit
TCATTCCACTCATATAAAGTACCTGGAAGACTCAAACCCATAGAGAATGC10.3508771929824561No Hit
CCTCCATGGCGCCCAGAACGCCATCCATCACGGCGGCGATCGGAACACCG10.3508771929824561No Hit
CAGTCCTGCAAGGTGTAGGGGGGTATTTCCTCGAAAGTCTCGGAGCTCAG10.3508771929824561No Hit
CCTTGTGATTCACCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGT10.3508771929824561No Hit
GTCCATTAGAAACCAGTCTCCTCCCAATTGTCTGGAGTTTATGAGCCTGG10.3508771929824561No Hit
TAATCATCCCCCTCGCCTGGCGCCGCGCCAAGGGCGGAGCTTGGCACAGC10.3508771929824561No Hit
AGCTTGGCCACCACATACTCGCCCCGGCTGCTGGGTTTGGGAGCCACGGC10.3508771929824561No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAA10.3508771929824561No Hit
AGATGGGGTTTCACCTTGTTGGCCTTGTTCATGTCTTGAACTCCTCCCTC10.3508771929824561No Hit
AGTCCCAGCTACTCGGGAGGCTAAGGCAGGAGAATCACCTGAACCTGGGA10.3508771929824561No Hit
TTTCCGTACATCTTCTGAATTCTAGGCGGTGGTTCCCCAACGTCAACTTG10.3508771929824561No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCAGA10.3508771929824561Clontech Universal Primer Mix Long (96% over 26bp)
GAAGAGCGTCGTGTAGGGAAAGATGAGATCTCGGTGTCGCCGTACCCATG10.3508771929824561Illumina Single End PCR Primer 1 (95% over 23bp)
CCTTTCATTTCCATTCGATGATGATTCCATTCGTTTCCATCCGAAGATGA10.3508771929824561No Hit
GTACTTTTTTTTTTTTTTTTTTTTTGTTTTGCGTTTATACCACTGCTTCC10.3508771929824561No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTCTGTC10.3508771929824561Clontech Universal Primer Mix Long (100% over 23bp)
TCATTGAACGGAATCGAATGGAATCACCATCAGATGGAAACGAATGGAAT10.3508771929824561No Hit
GCCTCCTGAGTAGCTGAGATTACAGGCACACGCCACCAAGTCTTGCTAAT10.3508771929824561No Hit
TTCTTTTGTTGCATAATAAATTGCTACAAATTTAGCAAATTAAAACAACA10.3508771929824561No Hit
GAGTACATGGGGAGGGCAGTCACATCTTTCCCTACACGACGCTCTTGCCT10.3508771929824561Illumina Single End Sequencing Primer (95% over 24bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.3508771929824561Clontech Universal Primer Mix Long (96% over 26bp)
CGTTTGATCTATCAGGTGTGATTTGCCTACTGGCTTACATCATGGAAATT10.3508771929824561No Hit
GCTCACAAGACTGGCTAAATAATCCGCAAAACCTTCTGAGCTAAAGGCTT10.3508771929824561No Hit
ATAAGAGACTCCACGTACAAACTCCACGACTTCTACTTCTAGTCAAGATG10.3508771929824561No Hit
GCCCACAGCCGCGCGCGGGCGCGCGGACTCGACATGCCCCGGCGTGCGGG10.3508771929824561No Hit
ACGCAGAGTACATGGGTCGGCGACCGAGTCTCCTCTTTCCCTGTCTCTTA10.3508771929824561No Hit
GTATCAACCCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10.3508771929824561Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCTCTGCTGTAAGACAGTAGACTCCTTCGATGAGGTGCTCAAAAATGCT10.3508771929824561No Hit
GTCTGATGGGAGCCACTGTTCCTCTCCACAGGATTTCTTCAAGTGGGCCT10.3508771929824561No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTG10.3508771929824561Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGG10.3508771929824561No Hit
CACCAAACACTGGCCAAATAAACTGAACTCTTATTCTTTATTCCTGACAC10.3508771929824561No Hit
GCCTGTTGTCGGTGCCTGGACGGAGCCTGCGGGGAACGCGCCATGAATGC10.3508771929824561No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCCCCATGTACTCT10.3508771929824561Illumina Single End PCR Primer 1 (97% over 40bp)
ATCCGACACGGCACCGGTGACCAGGGGCTTGTCGGTGCGTCGGCTGCTGC10.3508771929824561No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.3508771929824561Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGAGAGTACATGGGAAGCAGTGGTAT10.3508771929824561No Hit
GTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT10.3508771929824561Clontech Universal Primer Mix Long (96% over 27bp)
GGTGGGGACTGGGTATGCTTTTTATTCATGTTGCCATCCAAATCTTAGTA10.3508771929824561No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCTGTCTCT10.3508771929824561Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACTCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTT10.3508771929824561Clontech SMART CDS Primer II A (100% over 26bp)
GCCTGAGCCAGGACGACTTCCTGCAGTGGGGCTGGCGCATTCCGTTCGTG10.3508771929824561No Hit
CCAGGTTTGGGAAGTTTTCAGCTTTCATTTCATTAAATGTTTTCTGTGTT10.3508771929824561No Hit
CATAAGGACGTGTGTAAATATGTACATTTGTCTTTTTATAAAAAGTAAAT10.3508771929824561No Hit
GTACCAGCGAGTGTCATGCAAAAGCCAACAGTTTGAAACAGGAAGGGCAA10.3508771929824561No Hit
GCAGTGGTATCAACGCAGAGCTTCCCATGTACTCTGCGTTGATACCACTG10.3508771929824561Clontech SMART CDS Primer II A (95% over 23bp)
GCCCCGCGGATATCGAGGTCATGGCTGGCTAGCTGGAAACCGGCGCCCAG10.3508771929824561No Hit
GAGGCGGACTGTGCGTAGCTGTCCACGAACGTCCCTGCTCCGCTGCCTAG10.3508771929824561No Hit
GTGTAGGGAAAGAGTGTAGATCTGTCGCGTCACCCATGTACTCTGCGTTG10.3508771929824561Illumina Single End PCR Primer 1 (95% over 23bp)
CAGCAAGGCATGGACGCATACCGCTACAACGCCGCATTCGTGGACGCGGG10.3508771929824561No Hit
GAACAGATTAAAACTTTAATTATCACTTGTCTTTCTGCTTTTCTCCTTCC10.3508771929824561No Hit
GCTCCTGTTTGGCAATAATTTCTGGAACTGAGGCACAACTGAATATTTTA10.3508771929824561No Hit
CCTTCCTGTCCATCGGTTTTCCACAGGGTGATTTGCACTGCCTGAGAGAA10.3508771929824561No Hit
ACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTT10.3508771929824561No Hit
ATATAAATAGTGTTTAGGGTCATTTCTGATCCCTATCACCAGATAGGCAA10.3508771929824561No Hit
CCCATGTACTCTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT10.3508771929824561No Hit
GCTCATCCACTATCAACAGAACCAGGCATCATGCGGCAAACGCGCAATCA10.3508771929824561No Hit
GTCAATACAGTTTCTACCAAAGTACCAATGTCATTCTTCACAGAGTTATT10.3508771929824561No Hit
GTCTTGCTCTGTCGCCCAGGCTGGAGTGCAGTGGTGTGATCTCGGCTCAC10.3508771929824561No Hit
GCCTTCTCCCTTCCTGTTCCCTCATCTCTGCCTTCCCCATGTACTCTGCG10.3508771929824561No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.3508771929824561Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGATGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC10.3508771929824561Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA10.3508771929824561Clontech Universal Primer Mix Long (96% over 26bp)
TGTCCTGCCCCTGCAGCGCGGAGACGACCTCTGGTCTCCCCACAGGGCGC10.3508771929824561No Hit
TTCCCAGTCTTTCTAATCACAATAAATTCCCACATCATTCATAGAAGTGC10.3508771929824561No Hit
CACGAGACTACTTTTCCCTACACGACGCTCTTCCGATCTCATGTTTACGG10.3508771929824561Illumina Single End PCR Primer 1 (100% over 27bp)
GAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10.3508771929824561Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACATTAATTTGCATCTCTAGTGCCCTCTCTTATAGTATAAATAGCACTCA10.3508771929824561No Hit
GACGAAATCCAGTATGGAGCGCCGTCGACGCAATACTGGACGGAACTGTC10.3508771929824561No Hit
AATGATCCGCTTCAGCCTCCCAAGGTGCTGAGATTACAGGTTTGAGCCCC10.3508771929824561No Hit
CTGCAAGCTCCACCTCCCGGGTTCATGCCATTCTCCTGCCTCAGCCTCCC10.3508771929824561No Hit
AAGTCGGAGCACTTCGCGCGACCGCTCGAGGACGAGACCATCGAGTCCGT10.3508771929824561No Hit
AAATACTGGGATTACAGGTGTAAGCCACCGTACCTGGCCTCTCTTGATTA10.3508771929824561No Hit
ATTATCTGCTTATTTATTTAAGTCCTTGTTGCTAGTCTGTTTCCTTCCAC10.3508771929824561No Hit
GATTATGGGCGTTGATTAGTAGTAGTTACTGGTTGAACATTGTTTGTTGG10.3508771929824561No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATACACATCT10.3508771929824561Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGGAATAAAACCAATTGAATCTAGTTTCTTTCAAGGCCAAAGGCTACCT10.3508771929824561No Hit
TCATAGCTATGCCCTAACTGTGTAAGTTCTTCATCGTAGTGAGTCATACG10.3508771929824561No Hit
ACATAAAAAAAAAAAAAAAAAAAAAAAAAAAATTTCACCGGCGTTTATAC10.3508771929824561No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10.3508771929824561Clontech SMART CDS Primer II A (96% over 26bp)
GTCGAGGCGCACGCCTCGATCAGCCGTGCGCTGCTCGGCCTGCTGCGCCA10.3508771929824561No Hit
CCGAAGATCGCCGCGACGGTCTCCAGACGCTTCTCGAGGTAGATCGCGAC10.3508771929824561No Hit
TTGTTAGATATGGGGAGTAGTGTGATTGAGGTGGAGTAGATTAGGCGTAG10.3508771929824561No Hit
CTGAAATGTAGATGAATAAGGTTCAACCCCAAATGGAGGGTATTTTCAGT10.3508771929824561No Hit
GTACATGGGTATGGCGACCGAGCTACCTTTTCCCTACACGACGCTCTTCT10.3508771929824561Illumina Single End PCR Primer 1 (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGAACCAGGGGT10.3508771929824561Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGT10.3508771929824561No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTTAAAGTGGGTTTA10.3508771929824561No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT10.3508771929824561Clontech SMART CDS Primer II A (100% over 26bp)
CTTCTAGCTTCCTCTCCCAACTGCATCGCTCCTCCTCTGTTCCATAGATC10.3508771929824561No Hit
CAATAAGAGAAACGCTGGAAACTACACAAACACATGGAAATTAAACAACA10.3508771929824561No Hit
CCCTAGGAGTGCCTTTCCCTGAAAATGAAACCAAAGTAAATACAGTTTTT10.3508771929824561No Hit
CCACTGCGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10.3508771929824561Clontech Universal Primer Mix Long (96% over 26bp)
CATTAAAACCACTACCTTAATTTTATGTTAGGATGCTTTTTTTTTTTTTT10.3508771929824561No Hit
GATCCAATCACCTGCCACCAGGTCTCTCCCCCAACATTGGGAATTACAAT10.3508771929824561No Hit
ACATATATACCAAATAACTCAGCCTTCATCACCACCCTCAGCTCATCCAG10.3508771929824561No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.3508771929824561Clontech Universal Primer Mix Long (96% over 26bp)
ATGTATAACTTTTCCCCAAAAACACACCCCTTCCAGCCTGCTTTAGAAAG10.3508771929824561No Hit
AATTAAGGCAGCTGAAAAGTAAATTGCCTTCTAGACACTGAAGGCAAATC10.3508771929824561No Hit
GTGTTCTTTGGGGAGGAATTTTCAGTGAATTGCTTTGTTTCTGCATTTTG10.3508771929824561No Hit
ATTCCATTCTTTCCATTCCATTCAAGTTGAATCCATTCCATTGCATTCCA10.3508771929824561No Hit
CTTCCGATCTCTGGTACCTGCAGAACTTGGGACAGCGTCGTGTAGGGAAA10.3508771929824561No Hit
GTTAATTAGTGAAAGTATATGGACCCAGGCGGTGAAGTTTCTCCCTGGCA10.3508771929824561No Hit
GTCCAGTAAATACAGGAGCTTCCCTGGTTTAAAAGCTTCTGAATTCAGGG10.3508771929824561No Hit
CCGGCGAGGTTATCCGGCTCGTTGATCGCCAGCACCCCGATGATGTTCGC10.3508771929824561No Hit
GCAGATGTATGAGCTGGACTCCGTGTGCCCTGCCTCCCTGGGGTTTTCAA10.3508771929824561No Hit
CAATGATAGGGAACGTGAGCTGGGTTAGACCGTCGTGAGACAGGTTAGTT10.3508771929824561No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTCTGTCTCT10.3508771929824561Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATT10.3508771929824561No Hit
CGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCG10.3508771929824561No Hit
CATCGAGATCATCCGCGATCTGCGCCTCGGCGCTTTCGACGTGCTTGTCG10.3508771929824561No Hit
AGTGGTATCAACGCAGAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC10.3508771929824561Clontech SMART CDS Primer II A (100% over 24bp)
GGTTATGGGAGTGGATCCTTCATAAATGGCTTGTTGTCATCCCCTTGGTG10.3508771929824561No Hit
AGCCAAGATCATGCCACCGCACTCCAGCATGAGTGACAATCTCAGCTCAC10.3508771929824561No Hit
GCTCGTTTTTCAGGACCTGTTGGTACCAGATCAGAAGAGCGTCGTGTAGA10.3508771929824561Illumina Single End PCR Primer 1 (95% over 22bp)
ATCTCGGTGTCGCCGTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.3508771929824561Clontech Universal Primer Mix Long (96% over 26bp)
GTCAGCTTGCCCAGGTTTAGTCCACTCATGCAACTTTTCCAAAGTCAACT10.3508771929824561No Hit
GTGTAGGGAAAGAGTGTAGATCTCGTGGCGCGATCTCCCATGTACCTGTC10.3508771929824561Illumina Single End PCR Primer 1 (96% over 25bp)
CTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCA10.3508771929824561No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT10.3508771929824561Clontech Universal Primer Mix Long (96% over 26bp)
GGTCTTGAATTCCTGACCTCAGGTGATCCACCCACAGCCTCCCAAAGTGC10.3508771929824561No Hit
GGGCAATTAGATGAGAAACAGAAACCAAAGGCATCCTAACAGGAAAGGAA10.3508771929824561No Hit
GGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCCAAGGCAGGAGGA10.3508771929824561No Hit
AGGCACAGCTCGCGCTCGCCGCGTATCAGGGGGGGCTCGCCGTCAGCGCG10.3508771929824561No Hit
CCCAGGAATAACCTGTTTGAGACCTGTGGGTGCGTGCCACCCGCGAGAAA10.3508771929824561No Hit
CTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAG10.3508771929824561No Hit
CCCATGTACTGTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.3508771929824561Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGCCTGGGACATGCACTTGTAGTCCGAGCTGCTTAGGAAACTGAGATGGG10.3508771929824561No Hit
GATACACTCTTCCCTACACGACGCTCTTCCGATCTATCCCTGTTGGTACC10.3508771929824561Illumina Single End PCR Primer 1 (96% over 27bp)
AGTGAAAGGTGCCTTTTGTGGCTCACTGCAGCCCTGCATAACAGGCCACA10.3508771929824561No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)