FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46
Sequences flagged as poor quality0
Sequence length140
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAGTAGGTTGGTCGTGCCGGCGACCGTGAAGGGCAGCCCCGCAGAGA36.521739130434782No Hit
GCCATGGCCGCGCGGTCATCGCAGCCGGCAAGCAGTGCGAGGCTGATCAG36.521739130434782No Hit
GTCGAGTCCTGATAGCCAGGCGAACAGAGCGTGGGGTTGGCCAGCGCGGC24.3478260869565215No Hit
ATTCCAGCCGTATACCTAGCTTCTGCGTCCAAGTCACGATGGCTGTGCTC24.3478260869565215No Hit
GTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGT24.3478260869565215No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA24.3478260869565215No Hit
CCACCCTGGACGCCCAGGTGCTGCAAGCGCTGTCGCATGCACAACCGGCG24.3478260869565215No Hit
TCTCGGTGGTCGCCGTATCATTCCCATGTACTCTGCGTTGATACCTGTCT24.3478260869565215Illumina Single End PCR Primer 1 (100% over 22bp)
GGCAAGCAGTTCCTTGGGAGAAAATGGTTTGGGCAGATAGTCGTCCGCTC24.3478260869565215No Hit
GCCTTGCGCCCCTTCTTGTCGGCGTACATGCCCAGCACGATCGCGCCGAC12.1739130434782608No Hit
CACGTAAGGCTTGGACTTGTAGCCTTCCTTCTCGAACGCACGGCGCATCT12.1739130434782608No Hit
TCAAGGTGCCGTGCGCGCTGGTCGGCGCGGACCTGCCGCCGGGCGAAGAC12.1739130434782608No Hit
GTCCTGGACCTTGTACTCCGCAGTCATGCTGGTTCCTTTGATGATGCGAG12.1739130434782608No Hit
CACTTTCCGCGCGCGATGACGATGGCGCCCGACGGCAGCCACGTCTACGT12.1739130434782608No Hit
GACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGC12.1739130434782608No Hit
GGTGTGCTGCCAGTCCGACACGCCGCTGCTGTATCTGCAAAACACCACCG12.1739130434782608No Hit
TTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCAAATTGAGCTGGCT12.1739130434782608No Hit
GCTCAGCCCCATGACGGCTTGGGCCCAGGCAACTGCCAAGACCTTCACCA12.1739130434782608No Hit
CCCAGCTTGAGCGCGCATTCCACCGCATCCGGTGGTGATTCCAACTGGCA12.1739130434782608No Hit
GAAAACTAGCGCGACCCATCTGGTTCGGAGAAGGCTCACGTCCTGATTCT12.1739130434782608No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA12.1739130434782608No Hit
ATCATGGAGAGTGACGAGTACCCGCCTATGTCCGGGGGTAATACCATTTC12.1739130434782608No Hit
AGATGACCGAGGCATTCAGCGGGCCGATCACCTGCTGCCCCGTGCCCGGC12.1739130434782608No Hit
GTCGTTTTCATCGTTCGTGCACTCTCTTCAAGCGGCGCTCAAGACACCAC12.1739130434782608No Hit
GGTGCCCAGCGGCGCGAGCATCGCCGCCGAATCGTTCAAGGCCCATTGGA12.1739130434782608No Hit
CACTTTCCGCGCGCGATGACGAAGGCGCCCGACGGCAGCCACGTCTACGT12.1739130434782608No Hit
CAGCGCCCCGGCGATGATGAACCCGGTCGGGGCGCCCAACTGCCCGACCA12.1739130434782608No Hit
CAACCGGGCCGGCACGCACAGGCCCGAAGCCAAATGCGTGCCGCGCCCGC12.1739130434782608No Hit
CCCCAGCGTGACGGGCCTGCACCACGCCACCAAGGGCGACACCTTCAAGG12.1739130434782608No Hit
GTATTGGACGATTATCGATGGCGTTTCTATGCTGGCCGGACAAAACAGGA12.1739130434782608No Hit
ACCGCAATGCCGCCGGCCTCCTCCTGCGCAACCTGCACACCGTCAAGGGC12.1739130434782608No Hit
AACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA12.1739130434782608No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC12.1739130434782608No Hit
TCTCAACAGCGAGTGTATTTAGGACGCACGCAGCCCTATTACGAGGGTTG12.1739130434782608No Hit
CTGTCGGCCTGCGAGTACTGACGGATGATCCGTTCGTTCTCGTGCACGAA12.1739130434782608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)