FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33
Sequences flagged as poor quality0
Sequence length140
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTACATGGGATGAACGACTGCTACTCCAAGCTCAAGGAGCTGG39.090909090909092No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26.0606060606060606No Hit
CGCTGATGCCCTGGCCCTCCTCCCGGCAGAGTTGCCGGAGGGATGTCTAC26.0606060606060606No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26.0606060606060606No Hit
GTCTCGCTCACCCGCGCGGAAGGGCCGCAGCTGTCCTACGACAAGCCGTC26.0606060606060606No Hit
ACGAATGTATCTCCATCGTCTCAAAAGAAGCGGCAAGGACATGATGCAAA26.0606060606060606No Hit
CTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGC13.0303030303030303No Hit
GGATGCTGATATCCGTGTTGAGGGTGGTCAGCGGCGTCCTGGACGCCTGG13.0303030303030303No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13.0303030303030303No Hit
GGATGAAGACCCAGGCGTTTCCGGCCTGAAGAAGTTGAGCGAAGTTTTCC13.0303030303030303No Hit
GGACAGGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCT13.0303030303030303No Hit
CTATGCGATGATTCCATTCCTTTCCATTAGAAGATGATTCCATTCGAGAC13.0303030303030303No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT13.0303030303030303No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT13.0303030303030303No Hit
CTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGGGTCAATTTAAGC13.0303030303030303No Hit
CTCTCACACCTACGCCCCATGACCCTGGACTACCTTGATTTTGATTACAG13.0303030303030303No Hit
GCCTTCAGCCGCCCGTTGACTAAATCGGTCGGCGTTGCCATCGTGAGTGC13.0303030303030303No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA13.0303030303030303Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCCGAAGCGCGCGGCTGGAAGGTGGCGCAGGAGAAGAACGAGGAATAC13.0303030303030303No Hit
GGCACATGCTGCCCATTACCCGACTTTCTCGGTCCTGGTCTTAAGGGGAG13.0303030303030303No Hit
ATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA13.0303030303030303No Hit
GTAAAGTACGGAGCGAAGTTGCAGTTAAAGTGAGAGTGATGCAGGTCGTG13.0303030303030303No Hit
GCGATGTCCCAGATGGATTGCGCCTGCGCCCGCGCCATCTTCTCCATCTT13.0303030303030303No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT13.0303030303030303No Hit
GCATATCCACATTGACGGAATGCCGCTGCATATCATCGATACCGCTGGGC13.0303030303030303No Hit
ATGTAGTTCTGAATTTTGAACCACGTGGATGTATTACCTTACTAAACAAG13.0303030303030303No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)