FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003644038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11
Sequences flagged as poor quality0
Sequence length140
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA436.36363636363637Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC19.090909090909092Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC19.090909090909092Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG19.090909090909092Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTTTGAAACTAATTCCAGTTCTGCCTTGAAGCTAAGGTTGGAAATGACCA19.090909090909092No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA19.090909090909092Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT19.090909090909092Clontech Universal Primer Mix Long (96% over 26bp)
ACTAAATAATGAGGCACACAAATGTGAACAGATTGTTATTTTAAAGTAGT19.090909090909092No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)