FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003644307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15
Sequences flagged as poor quality0
Sequence length140
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGCAGCTCGCCAAAGACCGGTTCGTGGCAGAACTGGCTACCGTTTCCG213.333333333333334No Hit
GGCTGGGAGTACTGTGACGGCTTTCTGACAGCGCTCGTCTGCTGCCGCCG213.333333333333334No Hit
ACTGGAACCGCAACTTCGGCTTCCCCGGCGGCTTCCAGGAGCGCGCGACC213.333333333333334No Hit
AGGCAGTGGTGGCGTGCCATGGTCACCCGCCGCGATGACGTCGTTGGTCT16.666666666666667No Hit
CTCCCGCCGCAGCCGCCGCAACTCCTCCAGCTCGGCCGTCGTCAACCGGT16.666666666666667No Hit
GTGCTCTCCTTCCCGGAGAAGACGCGCTTCCAGGTGCTGGCACCGGTAGT16.666666666666667No Hit
CGGTTGGCGCCGCTGTCAGCATCGAGCCACTGCTGCGCCTTGATCACCGC16.666666666666667No Hit
ACTTTAAGACCTTCGTCGAAGTCTTTGACTTTAGTGAAGCCGACAACAGG16.666666666666667No Hit
GGCGGAAAGCCGTCGACCCCCCCCCCCCCGGCCGGGGGGGGGGGGGGGCG16.666666666666667No Hit
CATTGAGAAGGTGGATGGGAAACCAGTGGCCTCCGTTCTCGACGTCGGCA16.666666666666667No Hit
GTACTAGATTCGGCAGACAAGCCACCAAGGCCCAGCGTAAAGATCTAAAG16.666666666666667No Hit
TGGTGGCGATGAGCAACGCCGGCACCCTGCGCGATGCGTACACCGCCAAG16.666666666666667No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)