FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG310.714285714285714Clontech Universal Primer Mix Long (96% over 26bp)
ACGTTTACCGGCGCCGGGGGAGGGCAGCGAAAAGAGGAATCTTTCTTTCA27.142857142857142No Hit
AGCTTAAGGAGCTGGAACAGAAGCACTCGCAGCTGACCGAGGAGAAGAAC27.142857142857142No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT27.142857142857142No Hit
GCGTAACAGAGCAGAAATGCTCTTCATCGGTCCAACGCAGGCGATTGCCG27.142857142857142No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA13.571428571428571Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC13.571428571428571No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA13.571428571428571Clontech Universal Primer Mix Long (96% over 26bp)
AACCCTGACTTCCCTAATTCCCCCCATCCTTACCACCCTCGTTAACCCTA13.571428571428571No Hit
GTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCA13.571428571428571No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGAACACTGCGT13.571428571428571Clontech Universal Primer Mix Long (96% over 26bp)
CGCTGTGGGCCACGCTCACCACCACCTACGATGCCTTCAGCCGCATCGTC13.571428571428571No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT13.571428571428571No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG13.571428571428571Clontech Universal Primer Mix Long (96% over 26bp)
TGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA13.571428571428571Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13.571428571428571Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG13.571428571428571Clontech Universal Primer Mix Long (96% over 27bp)
CAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCAGAATATT13.571428571428571Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13.571428571428571Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC13.571428571428571Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG13.571428571428571Clontech Universal Primer Mix Long (96% over 26bp)
TCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA13.571428571428571Clontech Universal Primer Mix Long (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)