FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28
Sequences flagged as poor quality0
Sequence length140
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTTGACCATCATGAGCTCGCGCTCGGTATAGGAGCCCTCGGTCAGGT27.142857142857142No Hit
GTTCTTCAAGGTCCAGCATCTGCTGGGCCATCTTCTTCCTTTCAGCCTGT27.142857142857142No Hit
GACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATT27.142857142857142No Hit
GAGTATCGGCAACAACGAGCGGACGATCTTGCCCTTGTATTTGCCATCGC27.142857142857142No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA13.571428571428571No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA13.571428571428571Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC13.571428571428571Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG13.571428571428571Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TCGTAGGCCTGGGTGGTGGCCGCCGTCTGCATGACCTTGGCGTCGTACAG13.571428571428571No Hit
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT13.571428571428571Clontech Universal Primer Mix Long (96% over 26bp)
GTTAGGGTTAACGAGGGTGGTAAGGATGGGGGGAATTAGGGAAGTCAGGG13.571428571428571No Hit
GACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTG13.571428571428571No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13.571428571428571Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG13.571428571428571Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC13.571428571428571Clontech SMART CDS Primer II A (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT13.571428571428571Clontech Universal Primer Mix Long (100% over 23bp)
CTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGG13.571428571428571No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT13.571428571428571Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC13.571428571428571Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG13.571428571428571Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC13.571428571428571Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG13.571428571428571Clontech Universal Primer Mix Long (96% over 26bp)
TTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT13.571428571428571Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCGGTATCTT13.571428571428571Clontech Universal Primer Mix Long (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)