FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length140
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAAGAGTGCTACATAAAGCAAGAAACACTCAGTGGGTTGATTTCTAA24.761904761904762No Hit
CTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAATTA12.380952380952381No Hit
GCTGACACACAGCTAAGGAGCCTCATAGCCTGGCGGGGGCTGGCAGAGCC12.380952380952381No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAA12.380952380952381Clontech Universal Primer Mix Long (95% over 21bp)
GGTGAATAAATGAGAGATGCTCTGGGGGCAGAACAACTAGTACTTGGTGA12.380952380952381No Hit
GCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCG12.380952380952381No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC12.380952380952381No Hit
CAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGATCAGGGCGGT12.380952380952381No Hit
CCCATGACCTGGCGACAAACTGTGCAATGATAAAGGCAAACCCTGAGTGG12.380952380952381No Hit
ACATTAGGTTTTTGCTGGATTCATGCTTGGGGCAAGGGATGCATGCATAC12.380952380952381No Hit
CTAGAATATATGCAGAATCCTTTAAACAGTCGACTTCATATTTTAAACTT12.380952380952381No Hit
GGATGTGGCCATAGTGTTTGTCATCTATGATGAAGTGGTGAAGCTGCTCA12.380952380952381No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG12.380952380952381Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGAT12.380952380952381No Hit
ACGTGGTGGCTCACACCTGCAATCCCAGCACCTTAGGAGGCCGAGGCAGG12.380952380952381No Hit
TTGTAAGACACTAGCATTTGAACAGTAGGATGTGGCAATAGGATCTGGTA12.380952380952381No Hit
TATTACCCCAGGGATTTTTGTCCAGCATGTGGTTGCTGTTAAAGCAACAT12.380952380952381No Hit
GAAGGATTCATCATCATGGAACTTGCTTTTGAAAGCCAAATTTAGGATCA12.380952380952381No Hit
ATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATA12.380952380952381No Hit
CAGTGGCTCACACCTGTAATTCCAGTACTTTGGGAGGCAAAGCCAGGCAG12.380952380952381No Hit
CTATGATGCAATATGAATCAACTTCATTAATTGGACAAGTCCAGTGAGGC12.380952380952381No Hit
ATTCTAAGGAGGCTTGGTGCTCAGAAAGTAGTCCTCGCAGCGCCGTGAAG12.380952380952381No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG12.380952380952381Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAG12.380952380952381No Hit
CTTTGTCAAACACATTCTTGTATTGCTCTAGTGAACCTAGCACTGTGCCA12.380952380952381No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12.380952380952381Clontech Universal Primer Mix Long (95% over 22bp)
GTCCTCTGTCATGTTGCCATACTTGGTGGCATTCTGCATGGGCCCCTGGG12.380952380952381No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA12.380952380952381Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCCTACCCATTGACCAGGTGAAGACCAGCCATTTGGAGAATGACTACAT12.380952380952381No Hit
CATCAAACATTATGATGGCACACTGGGCTTGGATATAATAGCCATCTCTC12.380952380952381No Hit
TATCCACAAGGGAAAATAAAGAACTGAAACACTGCGATAGGAAAAGCTTT12.380952380952381No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCAAGTCTTA12.380952380952381No Hit
CCATGGCATATACCCGTAATCCCAGCTACCTGAGTGACCGAGGCATGACA12.380952380952381No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTGTTA12.380952380952381No Hit
GAACACCTCACGGATCCGACGGCCCGCCTCGGTGCGGATCGGGATGTTCT12.380952380952381No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT12.380952380952381No Hit
CTTACAATCATGGTGGAAGGCAAAGAGGAAGCAAGCACATCTTACCACCA12.380952380952381No Hit
GGTCCTGCTCCCTGGGGTGGCCAAGCATAGGTGGGTGCAGCCGCCATTGT12.380952380952381No Hit
GCAGGTGAACTATTGAGTCTCAGCATCTGTATAACTTGAAAGAACAATGG12.380952380952381No Hit
CTGTTCAAGTACAGGGGCCTGGTCCGCAAAGGGAAGAAAAGCAAAAGACG12.380952380952381No Hit
TAAGACAGTTATGCAAAATGGAGCTGGAGGAGCTTTATTTGTGCACAGAG12.380952380952381No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)