FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003644359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10
Sequences flagged as poor quality0
Sequence length140
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTTGGGGAGCCTTCCTTTCTCAGCATTTGCAGACTCTCTGAAGAGCC110.0No Hit
TCAGAGAATTGACACCTTCATGGCATTCAGTCTTTCCGTCCAAGAAAGGA110.0No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACGTG110.0Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACTCAATAAAGGTAAAAGAATAAGCAGAGGTAGATCTGGTTTTCTGAA110.0No Hit
GACAATGGGAGGAGTGTCAGTCTCTCTCCCCTCGGGCGATGCTACCTAAA110.0No Hit
GTACATGGGGACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTC110.0No Hit
GGCGCCATGCCGCCACCGGAAGGCAGCCACCAGTCGTCCAGGCGGTAGTT110.0No Hit
TTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAG110.0No Hit
GTATGTGAGTCACTTGAATGTTAGTTCCCTTCCTTGTTTAAAGGAGTACG110.0No Hit
GACGATGACTATTCATCATCTACGAGTTTGCTTGGTCAGAAGAAGCCAGG110.0No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)