FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003644371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG315.789473684210526Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATAGACACGCACTGGCAGGCCTTCCTGCCGCGCCTTGAGCTGCAGCGCC210.526315789473683No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGC15.263157894736842Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA15.263157894736842Clontech Universal Primer Mix Long (96% over 26bp)
GCCCAGTCTCTCCAGCCAGACTGAAGTCCCTTCACACCCAGGCCTCCGTC15.263157894736842No Hit
TTCTCGCGCAGCGTGTCGACCGCCGCGCTGACCTTCGCGGTCAGGGGAGA15.263157894736842No Hit
ACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCG15.263157894736842No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGAACACTGCGTTGATACCACTG15.263157894736842Clontech Universal Primer Mix Long (96% over 26bp)
GGCCATTTGGTCCGGGCTCTCCTCGGGCGCCGTGAGCGAGGATGACGCCG15.263157894736842No Hit
CATGTCGAGCATGCGGTCGGCTTCGTCGAGGATCAGCATCTGGAACTGGC15.263157894736842No Hit
GCGGGGTGTTGCAGGACCCTAGGAAACAAAAACTGTTAGAACTTTAGAGG15.263157894736842No Hit
ACCCGCTACCCGGTTATGCGGTTAGCCTGCCAGAACACTTTGCGGACGCG15.263157894736842No Hit
ATTTTAGCTAATTTGGAGATGAGTAATTTTAGCAAAGGATTCACACAGCT15.263157894736842No Hit
TCAAAGGCAAGTCTGCGCAACCTCTGCCATGCCCCGCTGACTTTCGCCTG15.263157894736842No Hit
GCACCGAGATCACCAAGGTGGCGACGATGGAGGGGCGGATCATCTCGGAG15.263157894736842No Hit
CCTTCCCTGACGTGCTGGGTTTGCTTATTGTATTACTCCTTTTATTCCCT15.263157894736842No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)