FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003644372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length140
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTATGGCTCCATGAACGGCTGGGTGGCCGACCTGATCAGCCAGGAGGT210.526315789473683No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA210.526315789473683Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA15.263157894736842Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACACACCAATGACCTAGCATCAGCACCAGGAAAGCAAAAATGACCACA15.263157894736842No Hit
ATCCGCCGCAACAAGTCGAGGTAGATCTGCATTCAGCGCCCCTCCAAATG15.263157894736842No Hit
GGATTTTAGAGTACTCGCTCTTCATGTACCCCTTAGAACTAGCCTCAGAC15.263157894736842No Hit
GTCGCGGTCAACGCGCCGTACATCGAGAACTCGTTCGTCGTCGGCGAGCT15.263157894736842No Hit
TGGTTAGTCGGTTCGTCGAGCAGCAGCAGATTCGGCCGCTGCCAGACAAT15.263157894736842No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT15.263157894736842Clontech Universal Primer Mix Long (96% over 26bp)
GCAGCGTATATCCACTAGGCAGTTGCCCTGATGCACCCGGGCTACCACCG15.263157894736842No Hit
GTTGCTTAGCAACCAGCTAATATTACAGCCAAAACAAACAGATCAATTGG15.263157894736842No Hit
CCTCTGCCCATTGCACCTATTGGCAGAGACCTCAGAACCTTAGGAAGGGC15.263157894736842No Hit
CTTGAACCCTATGCCCCTCTTCCGCGGCCCGCGGTCGAGCCACTGCCTCC15.263157894736842No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT15.263157894736842Clontech Universal Primer Mix Long (96% over 26bp)
GCTGGGACTATAGGCGCCCGCCATCACGCCCGGCTAATTTTTTGTATTTT15.263157894736842No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT15.263157894736842Clontech Universal Primer Mix Long (96% over 26bp)
TGCTGACCCCCACCCGCGAACTCGCCGCGCAGGTCGAAGAGTCGGTGCGC15.263157894736842No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)