FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33
Sequences flagged as poor quality0
Sequence length140
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGGGAATCTCGCTACGGCGGATCTCACTACAGCTCATCTGGTTACAGT39.090909090909092No Hit
CCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATC26.0606060606060606No Hit
TCTCCAAGTTGGTCAGGATGGTCTCAAACTCCCGACCTGAGGTGATCCGC26.0606060606060606No Hit
CTCCTAGACCCAGGGAGGCGGCATACTGCTGGCTGAGCAGGGAGCTGGCC26.0606060606060606No Hit
CTGCTTGCTCACGGCCAGCGTGGCCGGGTGCAGGCGCGTGCCGGAGCCAC13.0303030303030303No Hit
CAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCG13.0303030303030303No Hit
TGTTCGACAAGGACCTGGCGGCGTCCGACGAGATCACGCTCGACCAGTGG13.0303030303030303No Hit
CCCTCCAGAATATGTATGGCTGCGACGTGGGGCCGGACGGGCGCCTCCTC13.0303030303030303No Hit
CAACTGGCATCCTCACAGGTCGATATCCCGCACGTCTGTAGGGGTGCTGG13.0303030303030303No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13.0303030303030303No Hit
ATTGCACCTTGGCCGCCTGCAGCAGCCCGGGCGAAGCCAAGACCTCCGCC13.0303030303030303No Hit
CCAGCAGGAGGTACTTTGGATGACAAACCAAAGGAACAACTGCAGAGGAG13.0303030303030303No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGCTGTC13.0303030303030303Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTCTGGCTGGCAAGCCAGGAGAAGAAGGTGCGAGGCCGTGGGCGAGGTG13.0303030303030303No Hit
GATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCC13.0303030303030303No Hit
TCCCACCCCTGCTGAGATCCCTAGAGCTATTCAGAGCCTGAAAAAAATAG13.0303030303030303No Hit
GTAACCAAATCCAGTCTCTCATATCCCGTATTTTTTCTTTAGCTCTTCTA13.0303030303030303No Hit
GGGGAGGGCACGAAGCTGGCGGGACAACACTCCATGCTCAGCTGGCAGGC13.0303030303030303No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAATG13.0303030303030303No Hit
ACCTATCGCTGCTGGCAGCACTTTTTGAGCTAGCCGAGAGCACCCATTTT13.0303030303030303No Hit
GCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCC13.0303030303030303No Hit
AGCCACTGCGATGGTCTTTCCACGACCGGTATGACCGATAGCTAGCCCAG13.0303030303030303No Hit
CGGCATCACGCCCAGCGCGATCAGGCTCATGCGCTCAAGCGAACCGCCCG13.0303030303030303No Hit
GGCCTGGGGGTGGAGCCCTAGCCAGAGACCGTGCCCTTCCCTTCCCCCCT13.0303030303030303No Hit
GCGCTTCATCCCCACAGTGGAGTTCTTTGTTGTGAGGGGAGGGAATGCAA13.0303030303030303No Hit
TTCACTCCCATCAAAAGAAAAGAAAAGTCAAAAAAATATACAAAGCCAAA13.0303030303030303No Hit
GTATAAGGCTTTCCGGAGTGACAGTTCATTCAATTTCTTCGTTTTCTTCT13.0303030303030303No Hit
CACCCACACAGGTCGCCAGTCCTCCCAGTCGATGACCGCCAGCCCCGCAG13.0303030303030303No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)