FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33
Sequences flagged as poor quality0
Sequence length140
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTGGACCGCGAGCGAGACCTGGAGACCGAGGAGGACTTGGATCGTCG39.090909090909092No Hit
GGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGA26.0606060606060606No Hit
TTTATGAGTACATCATTTTCCGAGGAAGTGACATCAAGGATATCACTGTG26.0606060606060606No Hit
CTTTTATTCAGCACAGAACTGACTTTCTGTAGTTTTCCAGATGTGCCCTC26.0606060606060606No Hit
TGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAAT13.0303030303030303No Hit
GGCTAAGGCGCTTCGGACAGAATTTTCGGCCACGCGCCAGCATCGGCGCT13.0303030303030303No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTC13.0303030303030303Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGGAGCCACTCCACGCACTCGCCCTCCAGGTAGGCTCTCAGCTGCTC13.0303030303030303No Hit
ATCCAACTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGA13.0303030303030303No Hit
TCCTGGGGGAACCCGGGCTGAACTTTTCTTCTCCCCAGGGACCCTCCAAA13.0303030303030303No Hit
ACGACGGCCTTCCCGACCCCCGCGAAGCGGCCGCTGAATTCCCGGCTCGA13.0303030303030303No Hit
CAAGTGCTTGTTTGCAGTCTATATTTTCCACCACTTGGACTCACTAGCAT13.0303030303030303No Hit
CCTATAATCTCTCCGACATAAAACCACATCAACACCTCAGTGGCCACCAA13.0303030303030303No Hit
CCCCTTGTGCTTTCCACCCAGCCGGCCTGTCTGCGTCTGTGCTGAGAAGT13.0303030303030303No Hit
GCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAAC13.0303030303030303No Hit
ACTCCCCACACGCCTGTCAGGTTCAGCAGTCATGGCCATAGGATTGGGAG13.0303030303030303No Hit
TGCGGGAGGTGAGGCGGGCTCTGCGTGCGCACCGCGCCTACACGCCCAAC13.0303030303030303No Hit
CCACCGAGATCTGGGTAGGAATTATCGGAACCCTGGTACTGGGGATCATC13.0303030303030303No Hit
CCCCAGCAGCGGGAGAAGCTGTCTCTGATCCGCAAGCATGCTCAGACCTT13.0303030303030303No Hit
GCCCCCGTACCAGCAACAGAGCCCGTTGGACGACCGAGAGTTCAGCATGC13.0303030303030303No Hit
AGCGTGGGACGTGCCCACACAGGACTGTGGCCCACGCCTCAAGGTGCCAC13.0303030303030303No Hit
GCCCACAAATGGCTGACAAATATTTATTGGGCACCTACTATGTGCCAGGC13.0303030303030303No Hit
TCCAACCCCTGCCTTGTCCACCATGACCACACCTACTCCCTCCCGCGGGA13.0303030303030303No Hit
ATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACATTCAAA13.0303030303030303No Hit
ACGAGGCGCAGATGCCCTTCGGCGGCGTCAAGGGCTCGGGCCTGGGCCGC13.0303030303030303No Hit
GGAGTCACCAGGGCACAGCCCTCCAGGCCCGCCTCAGGAAGGAATGAAAG13.0303030303030303No Hit
CTCCCTGGAGTTCTTGCAAGTCGGCCAGGATGTCTCAGGCTGAGTTTGAG13.0303030303030303No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTT13.0303030303030303No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)