FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTGCAGCATTCTCTTCTGCATTACCACCAATTTCACCAATCAATATG412.5No Hit
GGTCAGTTATGTTGCTAAATTACTTTTGGCATTAAGTTTAGGGAAAAAAA26.25No Hit
GCCTCTACCATTGCACGAAAATAGGTCCTATCATGGTCACCAATGACTAC26.25No Hit
GCTACTCGACCACCGCGCTGGTGCGGCGCATACGCCGCTAGATCGCTAGC26.25No Hit
ACGCAGAGTACATGGGCAAAACTGAGGGCAGATGAGGCAAAACAAAGTGC26.25No Hit
ATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTTTTCCACTTCAA26.25No Hit
GAAGAAGAGAAGCTCAAAGCCATTACTTCCAAATCCAAAGAAGACAGACA13.125No Hit
CCTAAGCCAGCCCCAGGGTTCCCCCAGGGAACTCAGTTTTCCCATATGTA13.125No Hit
ACGGAACAGGGCTGACGCCGCTACATATATAGGAAAAGGGAAGGTAGAAG13.125No Hit
GCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAG13.125No Hit
GGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA13.125No Hit
GCTTAAAGATAAACTGAATATTTGAAGAAAGAACTAGCTAGTAAGTCTAG13.125No Hit
TAAATAGGATTATCCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGG13.125No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC13.125No Hit
ACATTTTTAAAAATTATTTTATTTTTTCAGACGTGGTCTCACTATGTTGT13.125No Hit
ACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAAC13.125No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCTGTCTCTTATACACATCT13.125Clontech SMART CDS Primer II A (96% over 26bp)
GATCTTGGCTCACTGCAACCTCCGCCTCCCAGGTTCAAGCAATTCTCCTG13.125No Hit
GTTTTAAAAGTCTGGGGCCACAATGCAGTCTCTTCCCCTCACAACAGAGT13.125No Hit
GTGGAGCCTGCGGCTTAATTTGACTCAACACGGGAAACCTCACCCGGCCC13.125No Hit
ATGTTCATGGGTCCCAGTCCTCCTTGTTTCTTAGGTGTATTGACCCATGC13.125No Hit
TCGTAGGCCTCGACGAAGCGGGCGCGGCGGATGTCGCCTTCGCCGAAGAG13.125No Hit
CCCTACTCCCCCACCGACGTCTTCGACGTGGCGCCCCGGTCCATGACCCG13.125No Hit
GTGTAGTGCTCCTACACATAAATGGGGTCAAGTGACATCACAAATTAAAA13.125No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)