FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003644385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8
Sequences flagged as poor quality0
Sequence length140
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCTGGGAAAGAGTGTAGATCTCGGTGGTCGCGCTGTCTCTTATACAC225.0Illumina Single End PCR Primer 1 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACCCGAACGGTTGTATCGCGTA112.5No Hit
CCGTCAGCCGGCTTGGCTGCGGCAATCACCAAATCATCCTGAGTACCCGG112.5No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT112.5Clontech Universal Primer Mix Long (96% over 26bp)
CCGTCAGCCGGCTTGGCTGCGGCAATCACCAAATCAGCCTGAGTACCCGG112.5No Hit
CTCCATACATGGGTTTGGGTGAGCTCGTTTCGTCTGCCTCGCTCGTGGAT112.5No Hit
ACCAAGCATCAGCGGGGCTGCAAGTTTGGGTTATGATCGAGGCATACGAG112.5No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)