FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43
Sequences flagged as poor quality0
Sequence length140
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA36.976744186046512No Hit
CGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGG24.651162790697675No Hit
CCGGGGAAGACGGCCGTTTCCGCCTCGCCGTCGAAGATCTGGTCTGCGAT24.651162790697675No Hit
CGACTGCGTCGTCGACGACCGTGATCGCCGTCTGGTCGTCGATCGCATAC24.651162790697675No Hit
GGTCTTGACACTGTCGGTCCGGACGATGCAGGTGGGGCGGTGTCGGTTCA24.651162790697675No Hit
CAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG12.3255813953488373No Hit
CATCATCATCATCATCATCACCACTTTTTCACAAAAATAGGCACATGTGG12.3255813953488373No Hit
GCAGAGCTGGGAGCCGGCCCTCAGCATGACCACCGAAACTTTATTTACAA12.3255813953488373No Hit
GGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGT12.3255813953488373No Hit
CCATAATACTTACAGCAAGCTGTTTGGGTAGGTTTTCTGCAATACGGCTT12.3255813953488373No Hit
CATAAGGGGATGGCCATGGCTAGGTTTATAGATAGTTGGGTGGTTGGTGT12.3255813953488373No Hit
TTCTTACCACAACTATCCTATAAACACTGCAACTATTCTTTCCAAAAGGA12.3255813953488373No Hit
GTGTAACACAGTGACAAGAGAGCTTTTACAATTAAGTCTTCTAATACTGC12.3255813953488373No Hit
GCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCC12.3255813953488373No Hit
GGGTGATGGTCATCTTGGTCTGGGCCACCGCCCGGTTCCCCGCGACCTCC12.3255813953488373No Hit
CCTCCTGGATGCCCTCCCAATGCTGCTCAGCTTGCAGTCATGCAGGGAGC12.3255813953488373No Hit
AAACAGGGAGTACCTTCAGGATTGGCCTGTTATCTTCTTTAGAACTAAGT12.3255813953488373No Hit
GGTATCCACTTGATGTCCTTTTATTTGACTTGTTACCATTAGTACTCTCC12.3255813953488373No Hit
ACTCCAGCCTGGGCAACATAGCGAGACTCCGTCTCTAAAAAAAAAAAAAA12.3255813953488373No Hit
CAATATTACAGTAAAAAAAAAAAAAAGCTAGTGGCAAAAAGAAGTGCCAT12.3255813953488373No Hit
GCTGTGGCCGCTTCGCGACCACCGGGCGGATATCCTGCTGGGATCCGCCC12.3255813953488373No Hit
GAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATC12.3255813953488373No Hit
ATTTACCCGGCGCCCCAGGTTTCCACAGCTGTAGTTGAGCCCTACAACTC12.3255813953488373No Hit
GGTTAGCATTTCCACCCTTTTTTAGGCCACTATCTGATTCCGTTCTCCTA12.3255813953488373No Hit
CACCTGTACGGCGTCTTTCCTGGCGACAGCGTCACCGCCGAGTCGGACCC12.3255813953488373No Hit
AGCCGAAGCCCATGTGGAAGCCCTCGCGCACGCCGACGGCGGCGGCGCCC12.3255813953488373No Hit
GGGACTATCACGTCTACGCCATGACGCCCGACTGCCCGAACTGGCGTGCC12.3255813953488373No Hit
GTACAGCAAATGACTGAAAGGGACAGAAGAGGTTGCGCTGTGGCAAGCTA12.3255813953488373No Hit
AGCCAATGGGGCGAAGCTACCATCTGTGGGATTATGACAGAACGCCTCTA12.3255813953488373No Hit
CCCTCAATCCAGCCCCGGAGAAGCAAGTGAAAAGGGTGGGTCCCTCATAG12.3255813953488373No Hit
CCCCCCATAGGGGGCAGTGTCTTGTTCCTGCCAGCCTCAGTGTCTTGCTT12.3255813953488373No Hit
GGGCAAGGGGATGCAAACAATACAAAAATCAAAAGCTTATCTGGTATTTA12.3255813953488373No Hit
GCCGTGCTCCCCGACGGCACGCTCCGCTTCGGCCGCCCGAGCGCGCTCGC12.3255813953488373No Hit
CTGGATGCTGCTGTCGGGGCCGTCGTCGCGGGCCACCGTCTACGGGTGCG12.3255813953488373No Hit
GATCGACACCAAATCGATCGGGCGCCGGTGCAGATGCTCGGCGACACGGC12.3255813953488373No Hit
CGTTGCGGGTGTTGGCGCCCAGCCCCCATTCCGGCGTGTTGGTCTCTGTC12.3255813953488373No Hit
CCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTC12.3255813953488373No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)