FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003644396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCATCGATCAGCGCATTCGCATCGTTGGCAAACTGCGCGGCAGAATAC312.0No Hit
ATCTCACTGACCGGTGCGTTGCTGGTGCATCGGGGGATGCAACCGCTGCA312.0No Hit
GTGTAGCAGCAATGCTCATTAATGATGACCCGATTGGAAAACGTTTCATT28.0No Hit
GCGATATCTGATACAGATTGTTGGAATTCATTGTGGTCAGTCAAGATAAC28.0No Hit
AACATTGGCAATGTGCTCCCTGTGGGCACCATGCCTGAGGGTACAATCGT28.0No Hit
CCCTGTGAGTCAGGGGGCTCAGTCTCTTTGATAGGTTCATTTTCTTTATT14.0No Hit
CATTCGTAACGGAACCAGTCACCACATGTCGATATGACAAACCGCATCGG14.0No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14.0No Hit
ACAGAATACTGCCGTAGTGGTGCATCATATGCGCCAGACGGGGAATAAAG14.0No Hit
GCTCTGCGCTGCGGCCCCGGGCCACCCAGGCCAGGGGCAGCAGCCGGCTG14.0No Hit
CGTCTTCCACCGTGTGGTGGCCGTCGATGTGCAGGTCGCCCTGGCAGTCG14.0No Hit
CCCCGGCGCAGAAGTGGGTCTTCGTGCCCTACAAGCCGAGCCCGCCCAAC14.0No Hit
AGTCTCTTCTCAGGCGATCCAGATAAGTGGAAATGAGAGCCGGGTTTTCT14.0No Hit
CCCCGAGTACGGGTCGACCCGGGCAGGGGTGAGCAGGCCGATCTGGTCGT14.0No Hit
GCTCACCTGCCCGTTGGCCGGAAAGCTGGGCCGCCGGCCGTCTTCGAGGT14.0No Hit
GTCCAGGAGAGGCAGCCCATCTTGGCCGTGGATGAAGAAGGAAACCCACA14.0No Hit
TGCATGGAACGGTTCTAACGGGATTTGGCGCGAATGTCGCGGGTGGTGTC14.0No Hit
CGACCCACAGTCCAGTTCTTCCTGGTGGCCTTTGCCCTCTACGTGGGCTA14.0No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)