FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65
Sequences flagged as poor quality0
Sequence length140
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCTCTCAAAAATAACAAGTATTGGTGAGAAGATAAAGACATTGGAGC34.615384615384616No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAGTACC23.076923076923077Clontech Universal Primer Mix Long (95% over 22bp)
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA23.076923076923077No Hit
GCACCACACCACGCTCGCTTGGCAGTAACGCGAGCAGGGGGCGTCCCTAT23.076923076923077No Hit
GCTTCCCATGTACTCTGCGTTGACACCACTGCTTCCCATGTACTCTGCGT23.076923076923077Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ATCACAAACAGATGAGTCACTTTCCACAGGGCTAATGATTCTCTCCCAAG11.5384615384615385No Hit
ACAGGGAACAATTAATTTTTATTGAGGTGAGGATGCAACAAGTAAGATTT11.5384615384615385No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.5384615384615385No Hit
TATGCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCAC11.5384615384615385No Hit
AATCTGGTCACCTATTTTGGGCTAGAATTTCCACAGCTGTTACAGAGGCA11.5384615384615385No Hit
AAATTGTCTCTTTGCTGCAAAGCCTAGCTCAGAAGCACACTAGGAAGCAT11.5384615384615385No Hit
AGATATGCAATATTTATTAGATATTTTATATAAAGAGGAAGCATAGTGAA11.5384615384615385No Hit
ATTGTAAATAGAATATATGATCTCTTTATAGATAATAACGTCCCAGAATT11.5384615384615385No Hit
GTCATAACACCTATTGCATAGCATTACAGTGGGCATTAAATGCATAAAAA11.5384615384615385No Hit
GCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTTGCAAA11.5384615384615385No Hit
ACTCAATACAGTCCAATCAGCGAGAAAATCTTTGCCAAACATGCCATTCC11.5384615384615385No Hit
GAAAAGCATTCTCACCACGGTGAATGGGGCGCGGCTGTTTTCCGGGACCT11.5384615384615385No Hit
GTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGTC11.5384615384615385No Hit
TTTCAGTATTCTCGCCACATTACAGGTCCTTCAATGTGCATTTCTCATTT11.5384615384615385No Hit
CTGATGGTGTGGTCCGGCGTCACCTTGTGTGTGTTGCGGATCATCGCAAA11.5384615384615385No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT11.5384615384615385No Hit
AGGTACAATAAATGTTTGTATGTTGAATTAATGTGAAATGAGCCTGGGAA11.5384615384615385No Hit
ATAACTAGTAGGGTGACTGAATCAGTAAACAAAAGCCTCCCCCCAGCAAA11.5384615384615385No Hit
CACGAATCCAACGGCGTCACACTCATGCCACGCTGCATAGGTGTATGGTC11.5384615384615385No Hit
CCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGG11.5384615384615385No Hit
GGTTGGGGTTGAAATGTTAAATGCACCCAACCCCCCCCCCGAGGGGGGGA11.5384615384615385No Hit
GGTTGGTGTTTGACAAAAAAAAGCAAGCAAACCCCCCCCCCCAGGGGGGG11.5384615384615385No Hit
GCTTAAAAAAATCTATTCCTTATATGGTATGACACCATCAGTACTTAAAT11.5384615384615385No Hit
GGGTTTTGTTTGTTCTTGTTTCTCTAGTTCCTTGAGGTATGACCTTAGAA11.5384615384615385No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.5384615384615385No Hit
CCGCAGCTAGGAATAATGGAATAGGACCGCGGTATCCAGGCGGCTCGGGC11.5384615384615385No Hit
GATGTATGCTATTACACTTTTCCAGTGGAAGATAACAAAGCCCATATAAA11.5384615384615385No Hit
ATAGAAGCACTGCTGGGTTTTTTGGTTTTGTTTTGGTTTTGTTTTTGGGT11.5384615384615385No Hit
AGTCTTGTTCTGTTGCCCAGGCTGGAGTGCGGTGGGATGATCTCTCCTCA11.5384615384615385No Hit
CCCTGGAAGGGGCTGGTTCCGCGCGTGGAACTTCCCAGGGGACCTCAGAA11.5384615384615385No Hit
GGTGAGGGTTTATAGACAGGTACACACCTGGAGGAGTTGACAGGAAGTCC11.5384615384615385No Hit
CAGCTTAAGTCACTGGAGTTAATTCAAGGTGAACCTCTGAACAAAGATAA11.5384615384615385No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTA11.5384615384615385No Hit
CTCCAAGACACATAATTGTCGATTCACCAAAGTTGAAATGAAGGAAAAAA11.5384615384615385No Hit
GTCCACGACGAGACCAGAAGTGGCATGATAAACAGTATAAGAAAGCTCAT11.5384615384615385No Hit
GAGTGCAGTGGCGCGATCTTGGCTCACTGCAAGCTCCGCCTCCCGGGTTC11.5384615384615385No Hit
ATCCTCAAATACTATAAAATAATAAGCAAAAGAATGTTACTTCCACTCAG11.5384615384615385No Hit
GAAAGAGAGCCTTAGATAAGGCAGAGAAATCAGAGGTGTTTTCGAAGGTC11.5384615384615385No Hit
GCAGCTGGTCGGCCATGTTGCCGGTGAGCTCCTCGTTCAGGTGCAGCACC11.5384615384615385No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG11.5384615384615385No Hit
GACAGGGTCTCACTCTGTTGCCCAGGCTAGAGTGCAATGAAACGATCATG11.5384615384615385No Hit
TCCCTGGTTAGCTGTATTCCTAGGTATTCTTTTTGTGGCAAGTGGGGATG11.5384615384615385No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC11.5384615384615385Clontech Universal Primer Mix Long (96% over 26bp)
CAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAG11.5384615384615385No Hit
TTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTA11.5384615384615385No Hit
ACTGATATGAGCCAGTGCTGCTGCTCTCTACGGAGTGTGCTCGGCTATAA11.5384615384615385No Hit
TCTCGGCGCTGCTGCTGCTGCTGCAGGTCTCCTCTTGGCTCTGCCAGGAG11.5384615384615385No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAGAGTACATGGGA11.5384615384615385Clontech Universal Primer Mix Long (96% over 26bp)
ACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA11.5384615384615385No Hit
CAGTAGTGGCAGAGGCAGAAGTGGACTTATTGTAGATTGCAGTACAGATA11.5384615384615385No Hit
CTATTATCATTGAGTTCAAAGAACTTTTAATTTCCATTTTGATTTCATTG11.5384615384615385No Hit
CTTTTGCCATGTAACGTAACATACTTACAGGTTCCAGGGATTAAGGCACA11.5384615384615385No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11.5384615384615385No Hit
TCTCCACGCACACCTTCTCCAGCATCTGGGCAAACCTGATGAGGTCATGG11.5384615384615385No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)