FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATGTATTA22.73972602739726No Hit
GACGCCGCCGGTGTCGAAGAAGCGCGTGCCGGCGGCCAGCTTGTCGAGCG22.73972602739726No Hit
ATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGT22.73972602739726No Hit
GTGTGCCAGAGCGAGGCGAGGTCGGTCAGCAGGCCGGTGGTCCCGGCGAG22.73972602739726No Hit
GATGGCCGGTTTGCCGGCGAGGGCGCGCGCGACGGCGACACGCTGCTGCT22.73972602739726No Hit
TTGCTGCCGATCAGGTTCACCTCCCACTTCCACTGCCGCGCGCGCTGGTT22.73972602739726No Hit
GAGGTATACTGTCTTTTGCATAAGAATTATTTTTATGTCATTTAATTTAT22.73972602739726No Hit
CGGCCGGGCAGAGACACTCCTCACTTCCTAGATGGGATGGCGGCCAGGAA22.73972602739726No Hit
ATATAAAAGAGGAAATCAAAATCAAAACGTTGGCCCAGACTCTTAGAACA22.73972602739726No Hit
GCCAATAGAGGGTAGAGAGAATTGTTTGAGTTGCAGGAACAGCAAGTGGT22.73972602739726No Hit
CAGAAACCAAGAAAGTAAGAGTTCACACAGAAAGAAAATTCTGAGAGTGA22.73972602739726No Hit
GTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAA11.36986301369863No Hit
AGATTATTCTCCCTTCATCTTCTTCCTCCAAGGCCACCTCTAAGGTAACC11.36986301369863No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTTAACACAGCGA11.36986301369863No Hit
GTCTCATTCTACAAAGAGAGAGGTGGCCCCCCTTTCTTGACCTCCTCCTC11.36986301369863No Hit
GAGTACATGGGAAGCAGTGGTATCAAAGCAGAGTAAATGGGAAGAAGTGG11.36986301369863Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT11.36986301369863Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG11.36986301369863No Hit
CAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAATGCGTTTT11.36986301369863No Hit
CCTACACCCCTTATCCCCATACTAGTTATTATCAAAACCATCAGCCTACT11.36986301369863No Hit
GTATCAACGCAGAGTACATGGGAAGCAATGGTATCAACGCAGAGTACATG11.36986301369863Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CACCGGCCGAACGCATCACCGAGCGCGGCAGCTACAACCTGGCCGGCGTG11.36986301369863No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC11.36986301369863No Hit
TCTCGAGTGTGTTTAGGTGAGTGTATGACTCGGGACAAAACTAAGAGGAA11.36986301369863No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
GGTTTTGCCCAAGGGCCGCACAGGTGGATGAAATTAACCCGGGGTGACGA11.36986301369863No Hit
CACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACT11.36986301369863No Hit
GCGTTGATACCACTGCTTGACCCATGTACTCTGCGTTGATACCACTGCTT11.36986301369863Clontech SMART CDS Primer II A (96% over 26bp)
AATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATGTATT11.36986301369863No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
CAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAACTGCGTTTA11.36986301369863No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC11.36986301369863No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
GTGCCAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACG11.36986301369863No Hit
CCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGA11.36986301369863No Hit
GCAAAAGCAGTGCTAAGAGGGAATTTTATAGCAATAAACACCCGCATTGA11.36986301369863No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT11.36986301369863Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC11.36986301369863Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCACCATGTCAGCTCAC11.36986301369863No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGCTGTCTCTTATA11.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC11.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
TGCTGCTGAAGCACAATGTTTTGGTGCTTTCTTTTCTCATTTGTTAAAGG11.36986301369863No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG11.36986301369863No Hit
GTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAGAGTACATG11.36986301369863Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
GTTTCAATGTTCAAAGCATCCCGCTCAGCATCAAAGTTAGTATAGGCTTT11.36986301369863No Hit
GTCCTGGGTCATGAAACTTAATCCACAGCAAGCTCCCTTATATGGTGATT11.36986301369863No Hit
CTCTGGCTGGACGCGTCATTGTCAAGTGCCCTACCTCAGGTCGGCTGGAG11.36986301369863No Hit
CGCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGCTAGCAAAAAGG11.36986301369863No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCA11.36986301369863No Hit
CGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAG11.36986301369863No Hit
CAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAGAGGGGTTTA11.36986301369863No Hit
CATGTACTCTGCGTTGATACCACTCTTCCCATGTACTCTGCGTTGATACC11.36986301369863Clontech SMART CDS Primer II A (95% over 22bp)
CCACGCGCCCCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCCGGGA11.36986301369863No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAGT11.36986301369863Clontech Universal Primer Mix Long (95% over 22bp)
TCCAAACAGCAAAGACTCAAAAGAGATTCTGCATTTCACATCAGTTCACA11.36986301369863No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTATGTCTCTTATAA11.36986301369863No Hit
CTGTCAACTCCGTCTCATTGAGGGTCCTGAGGAAGTTGATCTCATCATTC11.36986301369863No Hit
CTTTATTTTTCTTCCTTTTGTAGCTGAAGCAGAAAGAACTGGCTCATATG11.36986301369863No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGTTTA11.36986301369863No Hit
TAGTTGGTGACAGATTTCTATCATGCCAGACCACTGACTGTTCAGTCTTC11.36986301369863No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)