FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACGCAGAGTACATGGGAGTACATGGGAAGCAGTGGTATCAACGCAGA34.10958904109589Clontech Universal Primer Mix Long (100% over 21bp)
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA22.73972602739726No Hit
TTCTAATATAGAGCTGCCAAAATTGCTCTCCTGCATTTTTTAGGTGTGCA22.73972602739726No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA22.73972602739726Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTCAGCAGCTCCGAGTGGGACGGCTGGAGCGTCGGTTCGCTCGCCGCCC22.73972602739726No Hit
CCATTGGGAAGTATGACTTGGCTATGCTATTTGCTAGTGACAACAGTTAT22.73972602739726No Hit
CTCCAAGCTGGTGCCGGCCGAGGAGGTGGAGGCGGCGGCAGCGCAGCAGT22.73972602739726No Hit
GTGCTCAATGTTGCCCAGGCTGGAGTGCAGTGTCGTGATCTCGGCTAGCT22.73972602739726No Hit
GTCTACGAGAACGTCGAGCTGCCCTTGACCTATCGTGGAATGGCCGCGAG22.73972602739726No Hit
ATTCAAAACAGAACGGAAATAAGTTTATATAACTCACTCATCCACTTTTG22.73972602739726No Hit
GGCCAAGGTCGACCTGTCCAACTTCGGCCAGCCGCTGGCCGGCAACTTCA22.73972602739726No Hit
GTGTTCTACAGTGGAGGGAATCAATGATAGTGTCTCTGCCGAATGCTACC11.36986301369863No Hit
CACTACTGATTAAACAAGAATAAGAGAACATTTTATCATCATCTGCTTTA11.36986301369863No Hit
GGCTCAGGTCTCTTTCTGCTCTACTGGGATGATGCAATGTCAGCAAGTAC11.36986301369863No Hit
AGACCATCCTGGCTAACAGGGGGAAACCCCATCTCTACTAAAAATACAAA11.36986301369863No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAATTACACT11.36986301369863Clontech Universal Primer Mix Long (95% over 22bp)
GCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA11.36986301369863No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGCT11.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
ATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCC11.36986301369863No Hit
GTACCGGCCGTGGGGGGGGTGTAAGTCAGGGGGGAATATTTTTTTAAAAC11.36986301369863No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.36986301369863Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAAAAAAAAAAAATTACTCTGCGTTGATACCTGTCTCTTATAC11.36986301369863No Hit
CACCACGAAGATGAGGCCGGCGGCCGTATGCAGCATCCAGGCCCCGTCGG11.36986301369863No Hit
CCAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCA11.36986301369863No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
GTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCA11.36986301369863No Hit
GAATTAGAATTGTGAAGATGATAAGTGTAGAGGGAAGGTTAATGGTTGAT11.36986301369863No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATGCCGTGAAAGAAGAAAGA11.36986301369863No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA11.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG11.36986301369863Clontech SMART CDS Primer II A (96% over 26bp)
GTACCGGCCGTGGGGGGGTTTAAAATGAGTGGGGGAAAATTTTTATAATA11.36986301369863No Hit
GTATCAACGCAGAGTACATGGGGCTCAGCATTTGGGGACGCTCTCAGCTC11.36986301369863No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTTCCATGTACTCTGCGT11.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
GTAGTAAACAATTTCATTGATTTTTCTTAAATCAAACCACCTCATACTCG11.36986301369863No Hit
GCATTGAGGTACCTGTACAGGCCGCTGTTCTTTATGTTGTTTCTCAGGCA11.36986301369863No Hit
GTCTGCCGCCGCTGCCCGGACCGTGCATGGCACAAGATCTGGTGGCTGGG11.36986301369863No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACCTGTCTCTTATA11.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
CCCGTGAATAGGAGGGCATGACACAGCAAGACGAGAAGACCCTATGGAGC11.36986301369863No Hit
TGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCC11.36986301369863No Hit
GCACAGAGCTGCACCTTCACTGTTTCACAACAATCATGACCAGGAGCAAT11.36986301369863No Hit
CAAATACAAGATATTATCATTAATATTCTTCCTTCTCTTCCTCACTTTTA11.36986301369863No Hit
ACCCAAGACCGCAGGCTGGGTGTCTGCAAGGCAGAGCTCCATCTCCACTA11.36986301369863No Hit
GCATTTAGGTAACTGTAAAGGCAGCTGTACTTTCTTTTGTTTCTCAGGCA11.36986301369863No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTA11.36986301369863Clontech SMART CDS Primer II A (96% over 26bp)
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC11.36986301369863No Hit
GCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCG11.36986301369863No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC11.36986301369863Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGCTGAGAATGAGTTTGTGGTGCTGAAGAAGGATGTGGATGCTGCCTA11.36986301369863No Hit
GCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGGTGC11.36986301369863No Hit
GTACCGGCCGTGGGGGGGGTGTGATTGAGTGGGGTATAATTTTTTAAAAA11.36986301369863No Hit
ATTTTATTGATTTATTTTATCATTTCAAAATACCAACTCTTTTGATTTGT11.36986301369863No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGT11.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA11.36986301369863Clontech Universal Primer Mix Long (95% over 21bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC11.36986301369863Clontech Universal Primer Mix Long (96% over 25bp)
TTGTAAGAGTAATTTGGACATCCTAAACATTCAATAAAGAAAGCTATTAA11.36986301369863No Hit
TCCTAAGAGAGGCTGGAGAGAAAGCCACAAACATTAAGAAGTGCCGGTCC11.36986301369863No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTCAAGCAGTGGTA11.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
AGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCA11.36986301369863No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)