FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003644430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length140
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGAGACCAGCCTGACCAAGATGGAGAAACCCCATCTCTACTAAAAAA28.695652173913043No Hit
CGCCCATCCCGGTGAGCGGCAGGCCCGCGGATGTGATGACGGCGAGCATC28.695652173913043No Hit
GCTGGCACAAGACCTTCTTACCGCAATTTACGGGTGCATCCGCAACGAAG14.3478260869565215No Hit
CTGCAGGGCCGCACCGGGCTGGCGCTCTACGTGGCCGGGCTCAACGCGCT14.3478260869565215No Hit
GAGGTGCGCGATTCTCCCGGCCCCGATGCAGGGGTCAGCCGCCGATCCGC14.3478260869565215No Hit
CGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACA14.3478260869565215No Hit
CCCCATGCCCTGCGCCTTCGCTTGGGTCAAGATCGCGTGCATCGACTTGA14.3478260869565215No Hit
CGGCACACGTCCGCCGAGCCGTCCGGGCTGCCGTCGTTCACGAGCACGAT14.3478260869565215No Hit
GCGTTCACAAGACTCTCGCTTCGGCTCGGTCACCCTCCCAGCAGGCCTGT14.3478260869565215No Hit
TTTAAAAAACAGGACATACATGGGTATAACCACCGCCACTACCCCCAGGA14.3478260869565215No Hit
GGCCTTCTATAGCCTCTGTTGTGACCTCGGAAATCCCGATTCTGATATAC14.3478260869565215No Hit
GCTCGACCCGGAGGCGCCGCCGACGGCGACGGAGCTTTTGCGGCTGCTGC14.3478260869565215No Hit
ATTGAAGCCAACTATCAGGGCCGACTGCTCGCCAGACAAAGCGGCTTCCT14.3478260869565215No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTC14.3478260869565215Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA14.3478260869565215Clontech Universal Primer Mix Long (95% over 21bp)
GGTTCTTTAAACAAATTTACTGAAGCAAAAGCTTCAAAGCTGGAACAACC14.3478260869565215No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG14.3478260869565215Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTAATGAAAGATGCTAAATTCATTTTTGATGAAAAATGTAATGACGCATT14.3478260869565215No Hit
GCGGTCACAAGACTCTCGCTTCGGCTCGGTCACCCTCCCAGCAGGCCTGT14.3478260869565215No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC14.3478260869565215No Hit
GTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGTGTCAAATTAAGCCG14.3478260869565215No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)