FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length140
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA47.6923076923076925Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTTCGACCACACGGTGAACGTCTACGGCAAGACCGAGGCCGAGGGCTT35.769230769230769No Hit
AGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAAC35.769230769230769No Hit
CGGCGGCCCAGACGAGGCGGCTGCTGGCTTGGGCCGATCGGCACGCAATG23.8461538461538463No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT23.8461538461538463Clontech Universal Primer Mix Long (100% over 23bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA23.8461538461538463Clontech Universal Primer Mix Long (96% over 26bp)
TCTTCATGGGCGGCGGCAAGGCGCTGGTCAATGCCTATTACCGCAGCGCC23.8461538461538463No Hit
GTGTGAGTGCCATGCGTGTTGGGCTGCCGCCTCGGCGGCAGCGGGTCATG23.8461538461538463No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC23.8461538461538463Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG23.8461538461538463Clontech Universal Primer Mix Long (96% over 26bp)
AGATGGAAGCGCTCCGCGAAGCGCACGCCCGGCTCGCGCACGTCGAAGGC23.8461538461538463No Hit
ACCCCGTCTGGGAGGTGAGGGGCGCCTCTGCCTGGCCGCCCCGCCTGGGA11.9230769230769231No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAATAAAAAGGGTTAAAGTT11.9230769230769231No Hit
CCTTCGTCGCGCGCCCCATTGGCTCTGCCGTTTTTGGTCATTTTGGCGAT11.9230769230769231No Hit
GTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTTCTTTC11.9230769230769231No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC11.9230769230769231Clontech Universal Primer Mix Long (96% over 26bp)
GGAAATAAAATCACTATCTCAAAGAGGTATCTATAGTCCCATATTCAATG11.9230769230769231No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATAC11.9230769230769231Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC11.9230769230769231Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.9230769230769231Clontech Universal Primer Mix Long (96% over 26bp)
GCTTTCCATGTACTCTGCGTTGATACCACTGCTTTCCATGTACTCTGCGT11.9230769230769231Clontech Universal Primer Mix Long (96% over 26bp)
AGCGCGGAGTTCTCCAGCGTCAGCACGAACTGCTCGTTGGAATCGGTGAA11.9230769230769231No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC11.9230769230769231Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGATCTGT11.9230769230769231Illumina Single End PCR Primer 1 (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG11.9230769230769231Clontech Universal Primer Mix Long (96% over 26bp)
ACATAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACTCTGCGTTTATAC11.9230769230769231No Hit
ACGCAGAGTACATGGGCCTGACTTGACTTCCTGTCTCTTATACACATCTG11.9230769230769231No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11.9230769230769231Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC11.9230769230769231Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGTCTCTTATACACA11.9230769230769231Clontech Universal Primer Mix Long (95% over 21bp)
GAGCGCAGGTCGCCGGGATTGCCTGCGCTGGCGGCGAGCGCGAAGAAATC11.9230769230769231No Hit
GTGTAAAGATGCGAGGTGAAAGACCACGAGCAAAGCCACGGAGACCCTCT11.9230769230769231No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA11.9230769230769231Clontech Universal Primer Mix Long (96% over 26bp)
CCATTGCACTATCGTCACGATGTCCGACCGTAACTAGCGTACCTTCGAAC11.9230769230769231No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.9230769230769231Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC11.9230769230769231Clontech Universal Primer Mix Long (96% over 26bp)
CTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGATCCCTTCCG11.9230769230769231No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)