FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37
Sequences flagged as poor quality0
Sequence length140
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCTGTGCTGTCCCCTCGCAACTCCAGGATGGTGTGTAGCCCAGTGACT513.513513513513514No Hit
GTTCAAGGAGGAGTCCCTGCTGGCTCTGACGAGTATGAAGATGAATGCCC25.405405405405405No Hit
TAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACA25.405405405405405No Hit
GCCATGGGCCGTGTGATCCGTGGACAGAGGAAGGGCGCCGGGTCTGTGTT25.405405405405405No Hit
ATACACATCGTGACAGTCACCATTAACGGAGATGATGCCGAAAACGCAAG25.405405405405405No Hit
CCACAGGTCTCCTGCCCTTTCTCCACATTCCTGATCCATCTATCTACCAG25.405405405405405No Hit
GTACATGGGGTCATCACACTGAATACCAAAAAAAAAAAAAAAAAAAAAAA12.7027027027027026No Hit
GCTGTTGCTGTTGCTGCTGCTGCTGCTCCTTCTCCTTGAGCACTTTCTCT12.7027027027027026No Hit
AGGCTGGGCTGTCGGGGGCAGGACGAAGATTCTGGCTGAACTTTCCAAGG12.7027027027027026No Hit
GTTATAAACACCATGCAGTGTATTCGGTGGACTGTGCTATTTCTGTTTAT12.7027027027027026No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12.7027027027027026No Hit
TCTATATCCCATGCTTCCTTCCCATGGAGGAAAATGCTTAACACTGATCA12.7027027027027026No Hit
AGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCG12.7027027027027026No Hit
GGACCAGTGTGTGGAAATCACTGAGGAGTCCAAGGCTCTCCTGGAGGAAT12.7027027027027026No Hit
GGCTACAAGGACTCGCCCTCCGTCTGGGCCGCCGTCCCCGGGAAAACGTT12.7027027027027026No Hit
AAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAAAGA12.7027027027027026No Hit
ATATGATACGCGCACTGTCTATACATGGACGACACTTTGCACATCATAGT12.7027027027027026No Hit
GTATCAACGCAGAGTACATGGGGCGCGCGCCCGGCGTGGCAGAGCTGCGC12.7027027027027026No Hit
CCCAGGGGCACTGGCATCGTCTCCGCACCTGTGCCTAAGAAGCTGCTCAT12.7027027027027026No Hit
GCACAGAGGTGGTAGCAGCTTTCACCTCCTTGGGGGTAGGAGAGTTCCCT12.7027027027027026No Hit
GGTAAGCCCTGTCTTGCTCACTCCACGTATGGCAGTGCAGATGAAATGAG12.7027027027027026No Hit
TCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCTAG12.7027027027027026No Hit
CTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAG12.7027027027027026No Hit
GGCCTAGTATGAGGAGCGTTATGGAGTGGAAGTGAAATCACATGGCTAGG12.7027027027027026No Hit
ATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGC12.7027027027027026No Hit
CAATAGTATTCTTTGCTCTTATGCGATGGATGGACCCGTCCTGTACGCAC12.7027027027027026No Hit
GAGCCCCGTCTGTACAGTCTCTGGCCCCATGGGCACCAGGTGCCAGCTCC12.7027027027027026No Hit
AACCTGGATCTTGCATGAGGCCACCACCAATTTGCAGAGGAGAATCCACT12.7027027027027026No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)