FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37
Sequences flagged as poor quality0
Sequence length140
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAATAAGCGCCCATCGCTCAGGGCACAGGGCAATGTTGCGTTCGCACT25.405405405405405No Hit
GAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGC25.405405405405405No Hit
ACGATGTGGCGGGCGTATCGGATCAGGTCACGGTTGATTAGCGACGTCGT25.405405405405405No Hit
GCCTAAGGACGACGCCCTTAAAGCCCTTAAGGATGCCGGGTTCTCTGATA25.405405405405405No Hit
CCATGAGGATTAAGAAACAATAATGTAGGTAATGCACTTAGCACAGTGCC25.405405405405405No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12.7027027027027026Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT12.7027027027027026Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCCCGGGCATGACGATGACGATGCCCGCTGCAGCAAGGGAGGCCGGGCT12.7027027027027026No Hit
TTCAGTTACATTCTCCCAGTTGATTACATTCCAAATAGCTTTTAGATAAT12.7027027027027026No Hit
CCACCGGTACCCCGGCACAGGTGGCCATTCGGGCGGCTTCAAGTTTCGTG12.7027027027027026No Hit
GGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGT12.7027027027027026No Hit
TCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGTGT12.7027027027027026No Hit
GTGGAAATGGACGAGCCCAAACGGCCCAGCTGCCGTAGGATCGCAGCCCC12.7027027027027026No Hit
AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG12.7027027027027026No Hit
ATCTATACTCAAGCCATGTGGCAAGTAAAACAGATTTTTTCCACTGGACT12.7027027027027026No Hit
GCATGCGGGCCGCACGCATCTTGGCGACCTCCATGAAGAAGTTGGTGCCA12.7027027027027026No Hit
CCTCATGAAGTTGGTTTACGACGAGCTCATCGCCAGAGGGTTCTCCGATC12.7027027027027026No Hit
GACTGGGGGAGTGCGTCATTTCAGTGCTGCCTGCGCCCAGAGCTTGTCGA12.7027027027027026No Hit
ATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCA12.7027027027027026No Hit
CGGCCATGCACAGTACCGAAAGGCCAGCAATGCGAGCCCTGCGTCGGTGA12.7027027027027026No Hit
GACTATGCGAGTATCGTTCGAGCCTCGGGGATGGATTCGGTGACCCAGTA12.7027027027027026No Hit
TCCTCATACCTAGTCATGAACAGAATTAATGATTTAGAGGCATTAGAGCG12.7027027027027026No Hit
ATTATTTTATGTCATTTAAAAGAATGATGGAAAGGAATGTTAGCAGATTT12.7027027027027026No Hit
CTACTGAATACTATAAAATCAAAGAGTTCCCTTTTGATACGATCGACTTT12.7027027027027026No Hit
GTTCTGGACCCGTCCAGAACGGGCTGTCGACGCCATCGACCTGCGATCGG12.7027027027027026No Hit
CTGCCACATAGGTGAAGTCGTGTTCAACGTATGCGCGCAACGGCTGTGAA12.7027027027027026No Hit
ATAAAAGGCATCCAGAACACCAAAAGGGTAAAAATGCCAGAAATAAATCA12.7027027027027026No Hit
ACAAGATGGAGGCCATTCCCGGATGGCGGGCGGATCTCTCTCATTAAGAG12.7027027027027026No Hit
GTGAGATGTCATCTCAAAAAACAAACAAACAAAAAAGATATACTTCTACA12.7027027027027026No Hit
GTGTAGGACGCCGTTGGCCACTCGGTAACTCGAGACAAGGTCGGAACTGA12.7027027027027026No Hit
GATCTGGTGTATGTAATAGTCACTTTACTCTTGCTGCTTTCAAAATTCTT12.7027027027027026No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACACA12.7027027027027026No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)