FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37
Sequences flagged as poor quality0
Sequence length140
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCACCAACGACGGCAAACCAAAACCAGAAGCCCTGGCCCTATCAGACA25.405405405405405No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA25.405405405405405No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT25.405405405405405Clontech Universal Primer Mix Long (96% over 26bp)
GATGGCTCGTACATCGGGACGCATCCCGCGTCGACGTCTGGCGCGAGCCA25.405405405405405No Hit
TTGATGGCCGTTGACCTGTTAGCAATAGCCCCATAATCCGGATCCTGGCC25.405405405405405No Hit
GTAAAATGGGGGCATCTTTTCTGTTTTTATCCCTGATGGTTCTGTTTTCT25.405405405405405No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT12.7027027027027026No Hit
ACATGGATTCCGTGAATCTAAGACTGGCGCTCAACGGTCTGCCACTGCCG12.7027027027027026No Hit
AACTCGCGGTGCCATCAGTTATCGAGAATGACGCTCGTGCCGCGACAATC12.7027027027027026No Hit
AGTTTAAGGAGAAGCTGACGGCTGCATCTGTTGGTGTCCAAGGCTCAGGT12.7027027027027026No Hit
GTTTCCACCATGTTGGCCAGGCTGGTCTGGAACTCCTGGCCTCAAGTGAT12.7027027027027026No Hit
CTCCTCCCGAAGGATTCTCACCGTCGACGCTGACTGCGGTTGAGTCCAAT12.7027027027027026No Hit
GACCTAGCCGATGTTGGCGGGGTACTAGCGGTTAATGCCGCCAGAGACCT12.7027027027027026No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGG12.7027027027027026No Hit
CGCCAGAACCATTGCCAGGACGGCCGAAAATAGACGAAGCTAGCCTTGTA12.7027027027027026No Hit
GGCCTGAAGGCCAAGCACGTCGACGGCATGGGCGACGCGGTGGAAGTGGT12.7027027027027026No Hit
GTCTTGACATCGAGGACGAGGCTGTCGGTGCCCTCTGCGATCTTCTTGCT12.7027027027027026No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACA12.7027027027027026No Hit
GGACCATTCTTAATCCGTGAGACCATGTCGACGAAGGCGGTGTTGATCGA12.7027027027027026No Hit
GTAGAATAAACTCAAAGAGACTGACACTGAGATACATTATAGCTAAACTG12.7027027027027026No Hit
TTATATCATTTTGTGTTAATAATGACATTTACTGCATTAAAAACAGACAA12.7027027027027026No Hit
GGCTTTGATGAGCGCGCAGGGTGTCGGCGCCGAGGTTCGACAAATCCGCC12.7027027027027026No Hit
TTATAATCCAAATGACTGAAATGTGGCAGACAAAGAGTGAAGTCAAATAC12.7027027027027026No Hit
TCCCTTTCCAATACAGCCGGTAAAAATTGTTCGTCAATCAACCCTTGTTT12.7027027027027026No Hit
ATCTTCGCGTACACCAGCGCGAACATGCCGAAGTGGAACTCCATCTCGAT12.7027027027027026No Hit
TAGCCGAGCTGGTGCGCGCTCAAGCCCTCATCCTGCTCTCTGACGTTGAC12.7027027027027026No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC12.7027027027027026No Hit
ACAGCCCTAACCCTCGGGCGTGAGCTGGAGCGGTCGGGGTGACGAACAAA12.7027027027027026No Hit
GCAACTTGCATGGGTGTATAGGTATCTAGTTGTCCGATGGCAATTTGAAA12.7027027027027026No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT12.7027027027027026No Hit
GCAACAAAAACCTCTATACAAATGTAGTACAGCATACAAATTTTTAAAAG12.7027027027027026No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)