FastQCFastQC Report
Tue 10 May 2022
EGAF00003644587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387416
Sequences flagged as poor quality0
Sequence length140
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG20910.5397299027402069No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG16260.41970388419683236No Hit
GTATATAGGCTGAGCAAGGGGTGGTGAGGTTGATCGGGGTTTATCGATTA15330.3956986804881575No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC12210.3151650938526029No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG12180.31439073244264565No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG11610.2996778656534578No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC10140.26173415656555227No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC9260.23901955520680612No Hit
GTGTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTT8510.21966051995787475No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT8470.21862803807793174No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC8300.21423999008817396No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC8290.21398186961818821No Hit
CCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCG8170.2108844239783592No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA8150.21036818303838767No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC8060.2080450988085159No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG7600.19617155718917134No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT6870.17732876288021143No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC6730.17371507630041094No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT6710.17319883536043942No Hit
CAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCT6610.17061763066058191No Hit
TACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCA6360.16416461891093811No Hit
TTCCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTA6140.15848596857125158No Hit
GCATAGTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA5580.14403122225204948No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT5580.14403122225204948No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC5530.14274061990212072No Hit
CTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGC5410.1396431742622917No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG5380.13886881285233443No Hit
GTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACC5320.13732009003241993No Hit
GTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAA5280.13628760815247692No Hit
CACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCC5260.1357713672125054No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA5140.1326739215726764No Hit
CTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCATTCCAGTGAG5090.13138331922274765No Hit
CCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTAGG5010.12931835546286163No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA4980.12854399405290437No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT4890.1262209098230326No Hit
GTCTTTACGTGGGTACTTGCGCTTACTTTGTAGCCTTCATCAGGGTTTGC4780.12338158465318932No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC4620.11925165713341732No Hit
ATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCA4570.11796105478348855No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT4540.1171866933735313No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA4540.1171866933735313No Hit
GTACATGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGAT4520.1166704524335598No Hit
ACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAA4460.11512172961364528No Hit
GGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA4420.11408924773370227No Hit
ACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATAAGGG4400.11357300679373077No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG4400.11357300679373077No Hit
CCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACAC4400.11357300679373077No Hit
CTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATA4370.1127986453837735No Hit
CCTTTGAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAG4330.11176616350383049No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG4330.11176616350383049No Hit
GGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT4320.11150804303384476No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA4300.11099180209387326No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG4280.11047556115390175No Hit
GTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT4280.11047556115390175No Hit
TAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTTCGTAGTCTATTTTG4210.1086687178640015No Hit
GCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAAC4160.10737811551407273No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC4160.10737811551407273No Hit
GCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTAC4100.10582939269415821No Hit
GTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTA3980.10273194705432918No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)