FastQCFastQC Report
Tue 10 May 2022
EGAF00003644611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85603
Sequences flagged as poor quality0
Sequence length140
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA3760.43923694263051527No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT2770.323586790182587No Hit
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT2600.3037276730955691No Hit
GAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTT2180.25466397205705404No Hit
ACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCT1940.22662757146361692No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC1820.21260937116689838No Hit
CTATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCC1690.19742298751211992No Hit
CTCCTGCAATGCACGCTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTT1560.18223660385734144No Hit
TCCCAGAAGTGGTTGTTTCCCTTGCATGGGACGAAAGCTTGGCTCCAAAG1560.18223660385734144No Hit
CTGCAATAGCAGGCCATGTTTTTCCTTGAGCCTGGATGCTTTGGAGCCAA1400.16354567012838334No Hit
TCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTTTCTGCAA1390.16237748677032346No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG1370.1600411200542037No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC1200.14018200296718572No Hit
TAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTT1190.13901381960912584No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT1170.1366774528930061No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG1140.13317290281882643No Hit
GTACATGGGGCTCCTTGCTATAGAAGACCTGGGACAGAGGACTGCTGTCT1130.13200471946076656No Hit
GGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTT1130.13200471946076656No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1130.13200471946076656No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT1040.12149106923822764No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1030.12032288588016775No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA1020.11915470252210787No Hit
ATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTG1000.11681833580598812No Hit
CTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTTGAGCCTGGATGCTTT980.11448196908986834No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT980.11448196908986834No Hit
GGCCCAGGCTGAGCCACTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCC940.10980923565762882No Hit
GGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGCACGCTGGTATT920.10747286894150906No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT900.1051365022253893No Hit
GCCCAGAGTCTTCCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGC880.10280013550926954No Hit
ATATTAAGGTGTTCAGTTAAAATCTTTGAACCAAGCGCAATTGCTTCTTT880.10280013550926954No Hit
GCCATGAGGACCCTCGCCATCCTTGCTGCCATTCTCCTGGTGGCCCTGCA870.10163195215120964No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG860.10046376879314976No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)