FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTCTCACTGTGTCACCTAGGCTGGCCTGAAACTCCTGGCCTCTGGTGA23.9215686274509802No Hit
TATCTGAAGGCATAGCCCGCTTCCACAAAGCAATCTCAAATTTACAACAC23.9215686274509802No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA23.9215686274509802No Hit
GGTTTCAAGGACGGCATGAAGGACGGCTCCGCGTCCGACGCTTCCGCTGC23.9215686274509802No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.9607843137254901Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTTTTGCGTTGATACCACTGCCCCATGTACTCTGCGTTGATACCTGTCTC11.9607843137254901No Hit
ATCACGAAGTTACGGAGGCAAATTGCCGAGTTCCTTCAGCATAGTTCTCT11.9607843137254901No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.9607843137254901No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
CAATTCCATGATGAATCTATTTTATTCAATTGGTTGACATTTCCATTCGA11.9607843137254901No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTGAT11.9607843137254901No Hit
ATGCCGATTCCGTATATTTGAAATCTTTTGTTCTTTTGGCAAACGCACGT11.9607843137254901No Hit
GTCGAGGAAGCAGCGGTGCTCGGCCACCGCGGTGTTGCCCTCGAGGGTGC11.9607843137254901No Hit
GGGGTAAAATTAACGTAACGTCAGGCACCATATCAGTTGCTAACGATTCT11.9607843137254901No Hit
GTCTAAACCAAACCACTTTCACCGCTACACGACCGGGGGTATACTACGGT11.9607843137254901No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
ACCATGCAGTCCAGGTTGTGGAGCGCCGACGAGCCGCTGTAGATCATGTT11.9607843137254901No Hit
GTGTATATTTTACCTGCGTAGAGTCCGCATCACTCAAAGCGGCAGTCTGG11.9607843137254901No Hit
GGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAC11.9607843137254901No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA11.9607843137254901No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT11.9607843137254901Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
GTCTCATCACCGGAGCTGGCTCCGATGCTGAACGTGACGTCCAGCGTCAT11.9607843137254901No Hit
GGCCAGGAGTTGCGCAGCACAGAGTAGCCTTCCTCCTGGGAAGGGAGTGT11.9607843137254901No Hit
ACCTTGCCGGAAGCATCATCTGGGCGAATGAAGCGGCACTCGCGATGCAT11.9607843137254901No Hit
AGCCTATACCAACGACACGGACGTCTATCTCGCTGGCGAGAGCTTTGCCG11.9607843137254901No Hit
ATATTGGCCTGTAGTCTACTTTCTGTTGTGTGCTTGTCTGGTTTTGGTAT11.9607843137254901No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTGTA11.9607843137254901No Hit
CTGTCTTTGTAATGCAGCCGGCGGGATGGGCCGGCTGGAGATTGTCGGGA11.9607843137254901No Hit
AGGCGGATCCAGCCGTAGCCCGGCTCGATGATCTTGCCGCGGACCGACTG11.9607843137254901No Hit
GCCCAATACCATGCCGCTGTACGACGACCAGGAACTCTCGTTGAGCTGGG11.9607843137254901No Hit
GGTATATACATTGAGAAGCAATGCTATAAAGCATATGTCTTTGATCTGTA11.9607843137254901No Hit
GACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTATAAT11.9607843137254901No Hit
AGATAGGGTCAGCGTCCCCGCTGCCACGGAAGAAACTCGCTACGTTGGCA11.9607843137254901No Hit
GGTAGATGTCGCTCACGCGCAGGCCGACGAGATCACACCCGCGCAGCTTG11.9607843137254901No Hit
GACGACTCGCTCCCACCGAAGCCCTCGCTCGTGGGCATGTCCTTTTCGCC11.9607843137254901No Hit
AGATCTCGGTGGTCGCCGTATCCCATGTACTCTGCGTTGATACTGTCTCT11.9607843137254901Illumina Single End PCR Primer 1 (95% over 22bp)
GCCCGCGTGTGAACCCCATCGCGAGCCAGAGTGATGCCCGCTTCAGAACA11.9607843137254901No Hit
AAAAAAAAAGTGTAGATCTCGGTGGTCGCCCATGTACTCTGCGTTGATAC11.9607843137254901Illumina Single End PCR Primer 1 (95% over 22bp)
GTGCAGCGCCAGGTGCGGGTCGCTGGCATGTGCCGCGTCGAAATGGCCGG11.9607843137254901No Hit
GCGGGAGCCTGGGCCGGTGTCTGACGATCCCGACAGCAAGACACTCGCAC11.9607843137254901No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTT11.9607843137254901No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG11.9607843137254901No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11.9607843137254901No Hit
GATCTGGATGGCGGCCCGGGCAAGCCGGTGAAGAAGCCGGCGGTCACCGA11.9607843137254901No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)