FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA35.88235294117647No Hit
CGTTCACGTAGCGCTGCGCCTTGCCCAGCAGCGTGCCGACGGTACGGGCC23.9215686274509802No Hit
TGCTTAAACCGTTAGTTTGAATTAAAATATGTAACTGATGGAAACGGTTC23.9215686274509802No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG23.9215686274509802Clontech Universal Primer Mix Long (96% over 26bp)
ATTTTAGATGACTTGTTTTTGTACTTTATTTTTAAGAACTGTGCCCTCAG23.9215686274509802No Hit
ATACCAAAACCAGACAAGCACACAACAGAAAGTAGACTACAGGCCAATAT11.9607843137254901No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.9607843137254901Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAGT11.9607843137254901No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTAAAAAAA11.9607843137254901No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTG11.9607843137254901Clontech SMART CDS Primer II A (100% over 26bp)
TCGCCCCGCTGGCCGGGCTGCGCGCACGGCACGGCACCTTCGTGGTCACC11.9607843137254901No Hit
TATCAACGCAGAGTACATGGGATACGGCGACCACCGAGATCTCTGTCTCT11.9607843137254901Illumina Single End PCR Primer 1 (95% over 23bp)
CCCTGCATGCGGAAGATGCGGTTGTGGCGGCCATCACCGTGGCTGGTGTC11.9607843137254901No Hit
GCGCCAGACAATCCGGCTCGTCGTCGTCGCCGGTCATCACGACGTCACGA11.9607843137254901No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTAACAAAA11.9607843137254901No Hit
AACCTCAACTTTTGCAGGGAAGGTTACATTTAAGGTTTTATCTTTTAAGG11.9607843137254901No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC11.9607843137254901Clontech SMART CDS Primer II A (96% over 26bp)
TTCGCCGCCATCGCGGCATATGGATGTGAGGTAGCCGTCTCGCTGCAGAT11.9607843137254901No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTAAAACAA11.9607843137254901No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGTAGATTGGTTTGAACCCA11.9607843137254901No Hit
ACCCCGACCGATCGGGACCCGTCGGTGGACGTGGTCGTCCTGGACGGGCC11.9607843137254901No Hit
TCCCAGTACTCTGCGTTGATACCACTGCTTCCCAGTACTCTGCGTTGATA11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT11.9607843137254901Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCAAGGGTTCCTGCGTAAAGTTAATCTGCGCAGGTTTAGCCGGCCCCTA11.9607843137254901No Hit
GTATCAACGCAGAGTACATGGGCGACCACCGAGATCTACACTTTTTTTTT11.9607843137254901Illumina Single End PCR Primer 1 (96% over 26bp)
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAACTGTCTC11.9607843137254901No Hit
AGAGTGGCTGCTGGATTGACCATTACTGGGCGGGAGCGGATCTGAGAGAG11.9607843137254901No Hit
AGGCACCACCGGCCGATTCGTCGGCGTGGGCATCGAGATTTCCCAGGAAG11.9607843137254901No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC11.9607843137254901Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCTACATTTACATGAATTTTCTTCTTTTCTTGTGGTATCATTTGTAAA11.9607843137254901No Hit
GGTGCTATAGGGTAAATACGGGTCCTATTTCAAAGATTTTTAGGGGAATT11.9607843137254901No Hit
GTTTTATTCTTTAGCAAATGAAAAAGCCAGAGCTTATAGATGTTATCAAG11.9607843137254901No Hit
GTACGGTACGTATCCGTATGCTAACGACCCCGGTGGAACCTAAACCTGCC11.9607843137254901No Hit
AATCCGTCCGGCGGTCCGGCCCTGGCAACACATCCCGACAATCTCCAGCC11.9607843137254901No Hit
ACGCAGAGTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA11.9607843137254901No Hit
CCTCCATCTCGCGCACAATCGTCGCGTGCGGCGCCTCGTCCAGCTTGATC11.9607843137254901No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA11.9607843137254901No Hit
GAATCAAAGGGAATCATCATCGAATGGAATCGAATGGAAATGTCAACCAA11.9607843137254901No Hit
CGTCTCGACCTGCGAGCCACTCTGGTACGCTGGATTCAGATCCCCGTTCC11.9607843137254901No Hit
CGTTTGATGGCCCACTCTGCCAACGTAGCGAGTTTCTTCCGTGGCAGCGG11.9607843137254901No Hit
ACACCACCCCGATCAAGGCGCTGAGCAACCAGAAGTTCCGGCAGTTCCGC11.9607843137254901No Hit
TCCAGGGCCAGTGAGGTGCTTTGGATGGCCATGGCGGAAAGGGTATCAAG11.9607843137254901No Hit
GTGCGAGTGTCTTGCTGTCGGGATCGTCAGACACCGGCCCAGGCTCCCGC11.9607843137254901No Hit
TTATAATGATTATAAAAATTATGGATGTATAATTTTTAGATAATTTTATT11.9607843137254901No Hit
CTGGCTCCTGAACCCAGGAGCCAGTCATGCCCGAGGTCGTTACCGTTTCC11.9607843137254901No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)