FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length140
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGTAGAAGTCCTCCACGCTGTAGTCGGCCACGCCCGCCTTCATCGCG25.714285714285714No Hit
CCTTCATATTTGGCAAGGATTCTGCTGATGAGTTTTTTTCATCAGTATGC25.714285714285714No Hit
AGCTGAAGGAAAAGATTGATCGCCGTTCTGGTAAAAAGCTGGAAGATGGC25.714285714285714No Hit
ATTTCATATTGCTTCCGTGGAGTGTGGCGAGTCAGCTAAATACTTTGACG25.714285714285714No Hit
TCCCCATCCTCGCCCCCGCCGGCACGCCGGCGCCCGCGGTGGCCGCGCTG25.714285714285714No Hit
TTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGC25.714285714285714No Hit
CCCAAAGACAGCAGAAGAAAAGAAATAACAAATAACAGACAACTAAATAA12.857142857142857No Hit
AGTTGAATCCATTCCATTGCATTCCATTCCATTCGATTCCATTCGATTGC12.857142857142857No Hit
TTCACACACTTACTGCTGATTTGAATCTTATTCTCAGAGAATATACATCA12.857142857142857No Hit
GTTTTTTTGTGGGAGCTTGCTGCCCGTTTCTATTGCTTTAGCTCTGCTCT12.857142857142857No Hit
ACATCAATCACAGTGACAAATTTATAGGTTCGGCAAGTTCCACTGTGGCT12.857142857142857No Hit
GGCTGGTCTTAAACTCCTGACCTCAGGTGATCCGCCTGCCTTAAAAAAAA12.857142857142857No Hit
TATGGGGTGCAGGCAGTGTCATCTCTCTGACAAAATTTTTCAAATTTTCA12.857142857142857No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA12.857142857142857No Hit
CCAACATGGATAAACCCCGTCTCTACTAAAAATACAAAATTAGCCAGGCG12.857142857142857No Hit
TGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTA12.857142857142857No Hit
CAATTATTCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC12.857142857142857Clontech Universal Primer Mix Long (96% over 26bp)
AAAATACAGTTTCCAAAGTGCTCAATCAAAAAAATGGTTCAACTCTCTGG12.857142857142857No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTACTGTCTC12.857142857142857Clontech Universal Primer Mix Long (96% over 25bp)
GTATTGAATTGAAACACCTTTGTTTGGAATACATGACTCCATGGCTGTCA12.857142857142857No Hit
TAAATATCTGAATTCTGGAGGAAGCCCTCATTTTCCTTATTCTCCCAAGA12.857142857142857No Hit
ACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTCG12.857142857142857No Hit
GATAATGGTATAAAATTTTAGCTTGAGAGCTAAACAATAGGTCCTATTTT12.857142857142857No Hit
GGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGA12.857142857142857No Hit
GTGCAGGATCCACTAGGTGAAGCCAGCTGGGCTCCTGAGTCAGGTGGGGA12.857142857142857No Hit
AAACTCTTCTTTCAAAAGAAATTAACCAGAAATAGATTAAAATGACTTTA12.857142857142857No Hit
GTTATCCGCTCGGAGTCGGAAAAAATCCGGTTCCGATCGGCACGGTCGCG12.857142857142857No Hit
GTCCTATACATGTGTCCTGGTGGAGCAGAGGGAGCGGGAGAGGACCACGG12.857142857142857No Hit
GTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACT12.857142857142857No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)