FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAGGCGGTCCGCCGCGCCCGCGGCGGCCGGAGCGCCGCCGCGCGGG25.714285714285714No Hit
ACAGGGAAAGATCTCCACCCCTCTCTCGGGGAAGAGACCTAGTTACATCA25.714285714285714No Hit
GCCTTCCTGTCCCGCCGGCAGCAGCGCTAGGCGGTCCCCGCCGTGTTCGC25.714285714285714No Hit
GGTGGATAGTCTGAGAAGCTCTCAACACACATGGGCTTGCCAGGAACCAT25.714285714285714No Hit
TATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCG25.714285714285714No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCTGTCTC12.857142857142857Clontech Universal Primer Mix Long (96% over 26bp)
CTGCAAAGGGATATTTGTGAACATTTAGAGGCATATCATGAAAAAGGAAT12.857142857142857No Hit
CCCCGCTCCCAAGATCCAACTACGAGCTTTTTAACTGCAGCAACTTTAAT12.857142857142857No Hit
CAACGCAGAGTACATGGGAATGGAATGGAATGGAATGCAATGGAATGGAA12.857142857142857No Hit
TAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCT12.857142857142857No Hit
AGCCGAAGGCGAATCGTCATAAGAGCAGAGCTAAAGCAATAGAAACGGGC12.857142857142857No Hit
GATACAGAGTGCTGATTGGTGTATCCACAAACCCCAAGCTAGACACAGAG12.857142857142857No Hit
AAATAACCGTCCTTGCCCGGTTCGCGATATGTCAGCAGGGAAAGTCCGAA12.857142857142857No Hit
GTCTTTAAGATCCAAAAATATCCTTAAGTCTTTCTTTTTGGAAAAAGTTT12.857142857142857No Hit
CCCCGACACGTCGACGACCCATACGAACGTCTCCCCTATCAACATACGAT12.857142857142857No Hit
CCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGAT12.857142857142857No Hit
CGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACG12.857142857142857No Hit
TCAGTCTTTTTAAACACCGAATCAGTGGTTTTTAAACTAGGTTTTGCTGA12.857142857142857No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG12.857142857142857Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CACTTTCCCTGCTCATTTTTTTATTTTAATGCTTAGGTTCAATCTGCTCA12.857142857142857No Hit
CTTCAGGATTTCTATTTATTTTTCTGATTGAGTCTAGGAGGATTGTATGT12.857142857142857No Hit
GTGGAAGGCTGGCAGCGCCACAAGTTATGAAAACAATGAATGAATCAATA12.857142857142857No Hit
TTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGC12.857142857142857No Hit
GATCTGTAATCTGCCCAAGGCTTCCCCATATTTTTGCTTGAATAGATGGG12.857142857142857No Hit
ATCTGGTGCTGTGGGTCTCTCTCAGGCCTTGAGGGACACTTCCTTCCTCC12.857142857142857No Hit
TCTCCTGACCTTGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATT12.857142857142857No Hit
ATGTTCATCCTTGAAACAAATGGCAAAGCTATTCAGGATTGGTATTATTC12.857142857142857No Hit
ACTCTGATTAATAAAGAGAACTGTGATGATTTCTATAAAATTTTGAAATT12.857142857142857No Hit
ATCCTGATGGCAAGAGCTACAGCAGCGGCGGCGAAGATGGTTACGTCCGT12.857142857142857No Hit
GGTGAGGTTTCCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTGG12.857142857142857No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)