FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71
Sequences flagged as poor quality0
Sequence length140
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGG34.225352112676056No Hit
CCATTGCTATCCATTCCAGTCAATTCCATTCCATTCCGTGCCATTCCTTT34.225352112676056No Hit
ATATACGAATTGAAGCATAGCATCACAAGAAAAAAAATCTTAAAATTGTT22.8169014084507045No Hit
GTTCATTGACTGTTCCATTCCATTCCATTCGATGATTCCATTCGAGTCCA22.8169014084507045No Hit
CAACAAAAAAATACAACTCAATTAAAAATTGGGCAAATAATTTGCATAGA22.8169014084507045No Hit
GCTATATGTAGAAAGCTGAAACTGGATCCCTTCCTTACACCTTATACAAA22.8169014084507045No Hit
GGAAACGAGAATATCTACACATAAGAACTAGACAGAAGCATTCTCAGAAA22.8169014084507045No Hit
GAGTCCCCAGGAGAAAAAAGACATCCCAGGAAGAGAAGGCCAAAGTATGT22.8169014084507045No Hit
TCAGCGGTTCGCTGGCGAAGGTCTCCTCGGCGGCCGCGCGCAGGCGGCGA22.8169014084507045No Hit
GACAAAAGCAACAAGCTCAGGGCTGACATCAAGATACCTCCCAGAAAGAG22.8169014084507045No Hit
CACCGACGGTCCGCCGCACCTGCTGCTGGGCGACGCGCTGACGCTGCTCG22.8169014084507045No Hit
AGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCA22.8169014084507045No Hit
TCTTTATCAACTGTGTAATCTATGTGTTCATGACTTCAAAAATTAACCAT11.4084507042253522No Hit
AAGTACAAGATACAGGAAGATGCCTTATTTTGTTATCCCAAACAATGGGT11.4084507042253522No Hit
GTTTAACTCTGTGAGTTGAATGCACACATTACAAAGAATTTTCTGAGAAG11.4084507042253522No Hit
ATGCAGTGCCAGATATAATCTTGATCTCCATGTTCGGCCTTTTCAATAAT11.4084507042253522No Hit
GGCACCATCTCGGCTCACTGCAACCTCTACCTCCCGGCTTCAAGCGATTC11.4084507042253522No Hit
GGGCTCAAGCGATCTGCCTGCCTTGGCCTCTCAAAGTGCTAGGATTACAG11.4084507042253522No Hit
CAGCGCATCAGTAGTGCGTGTCGAACGTCGCGGTCACCACCGCCTCGCCA11.4084507042253522No Hit
GTTGCTACCACTCAAACAGCCAGCGAAGGCTAACATGGTCATCAGAGATG11.4084507042253522No Hit
GGTAGCTACGGCGCCTGGCATAGAGTGCACTGAGGGTGAAGCAGGTAAAG11.4084507042253522No Hit
GTACACATTATTTCATCACTCAGATAATAACCATAGTGCCTGGTAGTTTT11.4084507042253522No Hit
TATAGTATCTATTCACTTAGGATTTTATGCAAATCATAACTGAAAGGTTA11.4084507042253522No Hit
TTTTTAAATACTGTGATAGCAGACAGCAATTTGTTACTCCTAAGATTCTT11.4084507042253522No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGAACATGGGAAGC11.4084507042253522Clontech Universal Primer Mix Long (96% over 25bp)
GAATGAAATGAGTGACCATATGGACATAGGCCCCTGCAGCCGCCACAAAC11.4084507042253522No Hit
ATCATCGTAAATGGAATCAAGTGGAATCATCGAATGGAATCTAAGGGAAT11.4084507042253522No Hit
CCATTACATTATCTAACTAGCTATTGTTTGTGTGTAGAAAGGCTTCTGAT11.4084507042253522No Hit
CAAACTTCTGGTCTCAAATGCTTCAGCCTCCTAAAGTTCTGGGATTATAG11.4084507042253522No Hit
GGTGTGGCTAGGCTAAGCGTTTTGAGCTGCATTGCTGCGTGCTTGATGCT11.4084507042253522No Hit
GTGTGACCACCCTGTCCAGCCTGGTGCGGATGATGTATTCGCGCGCCGGC11.4084507042253522No Hit
ACCAAAGGCCTCTTTATAATTGACTAAAAAAGCAAAAAACAGAGAGGCTA11.4084507042253522No Hit
GAAATAATGGCAATAGCCTACACATTCATTAAGCACAATAATTCCAAGTG11.4084507042253522No Hit
TCTGTGACTAGGACTTAAAGGAATATATTTGTTCACACTTTTGTTGGAAC11.4084507042253522No Hit
GATGGCAACACCTCATAGCCATGAAACTGAACAGAAAAGACTGGGACTTC11.4084507042253522No Hit
GTGACCATCCAGCCTTTCCCAATTAGACTTCCTCTCCTTCCACCCCTCAT11.4084507042253522No Hit
ACATACAGGTTTGCCTGGAAAAGCCAAATGGATCCAAAGAAAACATTCCC11.4084507042253522No Hit
CTCTGGAACCATGAACATATTTTATCGAAAGATCAACTTTGATGCGCTTT11.4084507042253522No Hit
CAGTGGCTCACGCCTGTAATACCATCACTTTTGGAGGCCGAGGTGGCCAG11.4084507042253522No Hit
GGAGAGCACATCCGGTGTTAGAAGCGCTGGTAGGCCTCGGAGAGGCGGGT11.4084507042253522No Hit
AGGTAAACGTGCACCATGGGGGTTTGTTGTACAAATTACTTCGTCACCCA11.4084507042253522No Hit
ATCGCGGGAAGCCCGGCCAGCGACGACGGCACGGTGAAGACATCGTTCAG11.4084507042253522No Hit
GCAGTGATTCTGTGTCAGTTGCTCCTAATAAATCCCCTTCCGTATATCCT11.4084507042253522No Hit
GGCTAACACGGTGAAACCCCGTCTGTACTAAAAATACAAAAAGTTAGCTG11.4084507042253522No Hit
GTAAAGGATCGTGGATCTGCCCTAAGAACGCTTAAAATATGTGCCCTCCT11.4084507042253522No Hit
GAGGAAAGAGTAGGTGCATTTTTGGCATCAGGGACATAAGTTGTTCTTGT11.4084507042253522No Hit
AATGCAAACATCACAAAGCACTTTCTGAGAATGCTTCCGTCTAGATTTTA11.4084507042253522No Hit
GCTCTGCAAACGGAAGAGAGCAGAGAGCCAGCTGGCTCTGCATTTTGAAC11.4084507042253522No Hit
GATTGGTGTAACTCAACCACGAAAAGTAGCTGCTATATCAGTTGCTCAGA11.4084507042253522No Hit
CTCTGGAACCATGAACATATTTGATCGAAAGATCAACGTTGATGCGCTTT11.4084507042253522No Hit
GCTGTCTGCCTTGGGCTCCCTGATATTGATGATTTGGCTGATGGCAACAC11.4084507042253522No Hit
CCTGTAATGTGTGCAAAAAGGAAATGAGGGGTGGAAGGAGAGGAAGTCTA11.4084507042253522No Hit
GGCCTAAGGCAGGAAATAGAGATCAGAGGCTAAGGCGCACTTTTGGTTGG11.4084507042253522No Hit
TTCTTGAATAATCCAGTGTACAGATGCAAATTTTCCTGATTGGCTTGCAA11.4084507042253522No Hit
CTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTATAG11.4084507042253522No Hit
ATTCTAAGTGAAGTACCAAGCACCTGAACTTGTATTGGATGGGCCATTTG11.4084507042253522No Hit
GCCGTAGGGGACCTCCAGTGCCTGCGGACCAGCATGTCCTTTGTGTTGAG11.4084507042253522No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)