FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71
Sequences flagged as poor quality0
Sequence length140
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC34.225352112676056No Hit
CAAAAATGCTCTCCCATTCTGTAGGTTGCCTGTTCATTCTGATGGTAGTT22.8169014084507045No Hit
GAATTGAATGGACACGAATGGAATGGAATGGAATGGAATGGACTCGAAAG22.8169014084507045No Hit
CTTTGTATTTATAAAATTATGGTGAAGAACTTAAGGTTTGCTCATTAACG22.8169014084507045No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGG22.8169014084507045No Hit
AGCTCGGGCACCTTCGCCGCGCCGCGGGTGTAGAGCACCCAGCCGACGGT22.8169014084507045No Hit
GTGTTTCAATTTCTCTGCCTCCTGACCAATACTTGTTATTTTCTATTTTC22.8169014084507045No Hit
GAATGGTCTCGATTGGAATCATTATCAAATGGAATCGAATGGAATCACCG22.8169014084507045No Hit
TTACAGGAGTTGGCCTGGGCCCTGCCCTGGAGTTTTGTATTGCTGTCAAC22.8169014084507045No Hit
GCCAGGCGCCGTAGCTACCTCTTTCTGGGAGGTATCTTGATGTCAGCCCT11.4084507042253522No Hit
CCCGTATCCTGCCTACAACAAGGAAATGATACCCTCATTAAACATTTATC11.4084507042253522No Hit
ATGTCCATATGGTCACTCATTTCATTCAGGCTGGCCTGCTGTCTGCCGTG11.4084507042253522No Hit
CTCTTATATTCATATTATTTTCTCATCTCCTCTTGTCTCCTGGCCATTTT11.4084507042253522No Hit
GTGTCATTGGTTAAAAAAAAATGTCATAAAGTATATCTGAAAAATAAGAC11.4084507042253522No Hit
ACTCTGGAGGCTGAGGCAGGAGAAACGCTTGAACGTGGGAGGTGGAGGTT11.4084507042253522No Hit
CATTAAATCTACAGAGAAGCATTCAGAGAATCTTCTTTCTGTTGTGTGCT11.4084507042253522No Hit
CCTACATGTTGAATTACCAATAAGAGAAACTGATTTATTCTTATTAATCT11.4084507042253522No Hit
TGAGTACACAAATCAGAAACTCGTTTCTGAGAATGCGTCTGTCTACTTTT11.4084507042253522No Hit
AGTACATGGGATGAATTAGAAGGGACTAAAGACGGAATGAGTTAAGGGCT11.4084507042253522No Hit
TCACAGAACACACCACCTGTAATGTGTGCAAAAAGGAAATGAGGGGTGGA11.4084507042253522No Hit
GTGTGTATACACACATATAAAATATATATGTTAGAGATACTATTCCATTC11.4084507042253522No Hit
GCGTTGGAGCAATCGTTCGAACTGGCCGGCATGGCGGTGATCGCCGAACG11.4084507042253522No Hit
ATTCAAAGGTGATTTCATTCAAGTCCATTCGATAATTGCATTCGATTCCA11.4084507042253522No Hit
GGGTAGATGCTCATATGAACTGTGAAGATATTGCCATGAACTTCCTGGTG11.4084507042253522No Hit
GGCTGATGGACATAGGCCCCTGCAGCCGCCACAAACATACAAAGGGCAAA11.4084507042253522No Hit
ACCAAATACTGCATGTTCTCACTTATAAATGGGAGCTAAATGATGAGGAC11.4084507042253522No Hit
TCTAGGGGCTGCCCACACTCCTTGGCTCACTGCCCTACATCACTTTGACC11.4084507042253522No Hit
ATAACATTAGCTGGGCATGGTGGCATGCTCCTGTAGTCCCAGCTACCCAA11.4084507042253522No Hit
CAATTGTTTTATCCTCGCTTAAAAAATTTCAAGAAGTGCAGCACTCAGAA11.4084507042253522No Hit
GTTTTATTTATTGTTGTTTATTTGTTTACTCTGTCGTCATGAAAAAGCAA11.4084507042253522No Hit
GTTCCAGTGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGAGTACAGGT11.4084507042253522No Hit
GTTGTACGCATGTCAGAGAGGTTGCAGGCTGTTTTCAATTTGTCAGTTTG11.4084507042253522No Hit
GCTCAGGGAGGGATCGGGCACCATGATGGCCTCGGTCACCTCGTACACAT11.4084507042253522No Hit
TGCATACACACCTCCCAACTATTCAACCTTGCAGCTGGCTTTGGCAGAGC11.4084507042253522No Hit
GAACTGTGACTTAATTAAACCTCTTTCCTTTATAAACTATCCAGTCTTGG11.4084507042253522No Hit
ATGTCCATATGGTCACTCATTTCATTCAGGCTGGCCTGCTGTCTGCCTTG11.4084507042253522No Hit
CAAGAACTGCTGTTGACTTTTCTGCCTGTGCTTCTGCTTATTTATCCCAT11.4084507042253522No Hit
GGTATATGATCATTCTAGTTACCGTCTCTAAAATTATTTATCCTACATAA11.4084507042253522No Hit
ATACCACTGCTACACATGTACACTGCGTTGATACCACTGCTTCCCATGTA11.4084507042253522Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG11.4084507042253522No Hit
GACGGGTTCGGGGCCGAGGTGAAGCGGCGCATCCTGATCGGGACCTATGT11.4084507042253522No Hit
GGTGCAGTGGCTCATGCCTGTAATCCCCATAGTTTGGGAGGCTGAAGCAG11.4084507042253522No Hit
GGTTCATGCCATTCTCCTGCCTCAGCTTCCTGAGTAGCTGGGACTACAGG11.4084507042253522No Hit
TGAGTACACAAATCAGAAACTCGTTTCTGAGAATGGTTCTGTCTACTTTT11.4084507042253522No Hit
CCACAGCTTTGCACAAAGAAAACTCCTCATGTGGATATGGTTTCCGTTTA11.4084507042253522No Hit
CGCCGTAGCTACCTAATTCTGGGAGGTATCTTGAAGTCAGCCCTGAGCTT11.4084507042253522No Hit
AGCGAGCGGCGCGATGCGGGCGCGGTCGTGTGGCAGGTGCCGGTGCCGGC11.4084507042253522No Hit
GGATATCTTCATATAAAATCTAGACAGAAGCATTCTCAGAAACTTCTTTG11.4084507042253522No Hit
GGATGTGAGTGGTAGATCACACCAGTGAGGTTGTGATTTTGATTAGAGTG11.4084507042253522No Hit
GCCTTCTGGCTTCCATTGTTTCTGCTGAGAAATAGGCTGTTATATTAATG11.4084507042253522No Hit
CCACAGCTTTGCACAATGAAAACTCCTCATGTGGATATGGTTTCCTTTTA11.4084507042253522No Hit
AGACCAATGGAACAGAATAGAGAACCCAGTAATAAAGCCACATACCTACA11.4084507042253522No Hit
GTTTGAGTTTGCTAGGCAGAATAGTAATGAGGATGTAAGTCCGTGGGCGA11.4084507042253522No Hit
GACAATAAATTTCCTATCAAGATTTGTATCTCCAGATGGTCTAGATAATT11.4084507042253522No Hit
GGGTGAAGCAGGTAAAGATCATTGCCGTGCCCATGAAAGCAGTGGGTAGG11.4084507042253522No Hit
ATCAATATCAGGGAGCCCAAGGCAGACAGCAGGCCAGCCTGAATGAAATG11.4084507042253522No Hit
CATATAACCCCGTCAACGCAGCAGCACCTGAAGAAGGTCTATGCAAGTTT11.4084507042253522No Hit
GCGTTGGAGAAATCGTACCAACTTGCCGCCATGGCACTGATCGCAGAACG11.4084507042253522No Hit
ATCAATATAAGGGAGCCCAATGCAGACAGCAGGCCCGCCATAATGAACTG11.4084507042253522No Hit
GTCATGAAATCTAAGAAGAGATCAATGCAGTGCCAGATATAATCTTGATC11.4084507042253522No Hit
GACTTGAATGGACACGAATGGAATGGAATGGAATGGAATGGACTCGAAAG11.4084507042253522No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)