FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length140
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCCTATGGAGTAGCCATTCTTTTATTCCTTTACTTCCTTAATAAACTT26.896551724137931No Hit
GGGCCGGAGAGGGGCTGACCGGGTTGGTTTTGATCTGATAAATGCACGCA26.896551724137931No Hit
CCAATATGCACTGTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTT26.896551724137931No Hit
GTTCACTGCTAGCCTCGCCAAGTACGCAGACCCAGTAGCTGACCTGCTGG26.896551724137931No Hit
ATGCACGCTTAACTATCTTAACAAGCTTTGAGTGCAAGAGATTGAAGAGT26.896551724137931No Hit
GGGCAGGGTTGGGGGAGGTAGGAGACTCGGACCGGCAGCCCTGGCTCCAG13.4482758620689653No Hit
ATCACAAAGTTTCTTTGAATGTGAAATAGAGATACAGAAGAGTCAGTGTC13.4482758620689653No Hit
ACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGAC13.4482758620689653No Hit
GCAGGAAGCTGGGGTGTCGATGATGTCGATGTCCTTGGATGGCGGCTCAG13.4482758620689653No Hit
GGATTTTTTCGCCGCCCTTGAAAAAGCGTCCCCCGTCCCGATTGCATTTG13.4482758620689653No Hit
CTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAAT13.4482758620689653No Hit
GAGCTAGACTCCGTCTCAAAAAAAAAGGAAAGAAAGAAAGAAAAAGACAG13.4482758620689653No Hit
GAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAG13.4482758620689653No Hit
GACCTGAGTCACCAATACTGAACTGCACTTTTTGGGTAGTGCGGTGAAGT13.4482758620689653No Hit
GTTTATTAAGTATGAATTTTACAAACTTTACTTATATTAGCGGTAACGGT13.4482758620689653No Hit
GAATGGAATGGAACGGAATGGAACGAAATGGAATGGAATGGAATGGAAGG13.4482758620689653No Hit
CCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCG13.4482758620689653No Hit
CACCACGCAGAGCACAGCAGGAGACCAGGAGATGAGCACCCCACGATGTT13.4482758620689653No Hit
CTTCAGAGATGTTATCAATAAATTACACATGGGGGCTTTCCAAAGAAATG13.4482758620689653No Hit
AACATACACATTTCTCAGTGATTGTCCCCTCTTTGCCCTTTGCTGGCCAT13.4482758620689653No Hit
CTAAGAGATGATGAAAGCATCAGAGATGGCCAGGGGGCGGGAGGAGTGCA13.4482758620689653No Hit
CTGCTAATCATATACACAGTAGAAAAGACACATGATCTGGATTTTCTGTT13.4482758620689653No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA13.4482758620689653Clontech Universal Primer Mix Long (95% over 22bp)
AGCAGGATGTAGCCTGGAGGGCAGGAGCAGAAGTATGTGCCGGGCTGGTT13.4482758620689653No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)