FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length140
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTCACGAAGAACATGTGGGTCATGGGCGTCGGCGGGATCTCCGTCATG24.761904761904762No Hit
TGCTCAAGCAGGTCGACGGCGACATGTCCAGCCAGATGGTTGACGCCGTA24.761904761904762No Hit
CCCCAGAACCGCGCCATAAGCCGGAACAGGGTCGTCTTGCCAGCCCCCGA24.761904761904762No Hit
GTAAAAACGTCGTTGTCACAGATGTTGAAATTGAACAAACACGAAGCCGT24.761904761904762No Hit
TCGTAGAGCCGCAGTTCGGTGACTCGTTGCGGCGAGGTGTCGTGAGCAAG24.761904761904762No Hit
ATACCCTGCAGAACATCGCACGCGATGCCGCCCGCGAAGGCAAGACCGTG24.761904761904762No Hit
CAGCATCAGCGCGATAGTGCCGGCGGTTTCCGGTGTCGAGAAACTTGTAC12.380952380952381No Hit
TTGCATGGACGTCCTACCTGGGTCAGCGGCCCGAAGTAATCGTCATCGAC12.380952380952381No Hit
CGTTGGCACCCAGGTAGGGGCTCTCGGTGTGCGGCATGATGCGCTCGGCG12.380952380952381No Hit
CATCACAAACGCGCGCCACACCTTTTCGCGCAAATCGCGGCGCGGCGAAT12.380952380952381No Hit
CAGCCATTGACGCCGCATCGATGGCTACCCATGGAGCCGCAGATACTCTT12.380952380952381No Hit
GCCAACGCCTATCCGACCCAGCTGCGCGCCACCGCCACCGGCTGGGGCAT12.380952380952381No Hit
GTTTGAAGTAGAAAGAACAGAAAAAATTCAAGCAAGAGTGTTTTGTCACT12.380952380952381No Hit
GTGCCTTGATGCGGCGGCGCAGTTCGCCGAGCAGGTATTCGGTGGCGTCG12.380952380952381No Hit
CACAACAACACGGCTGCCGGCAGCGCCACCAGTCCGATCACCTCGATCCA12.380952380952381No Hit
CTGCTGGCAGGTGTTACTGGTGTTTTGATTACTAATATCGAGCGTAGCAG12.380952380952381No Hit
GTCCATCCCATCTTTTGCCCGGCTTCGTGGACAAGAGATTTAACGTTGAC12.380952380952381No Hit
TCTCTTTGTTGTCCTTGTCCTTGCGGTCTTTCGGGACGGTGATGAGCAGC12.380952380952381No Hit
GTCGGGCCATGGACTCGGTTCTCAGCGGTGACTGGTCCTTGAAGATGCAC12.380952380952381No Hit
GTCCAGCTATGGTCACGACGTGAGCCCGGCCGGTGGGGACGAGCACACCC12.380952380952381No Hit
TTGCATGGACGTCCTACCTGGGTCAGCGGCCCGAAGTATTCGTCATCGAC12.380952380952381No Hit
GCGCAGGATACGGGTCGATTGCGTGTTCATGATGTGCCCTCTCCCTCTTA12.380952380952381No Hit
CTCTCATTGGGTATATCGACTAGACCATCGCACCAATGAGTGACGATGTC12.380952380952381No Hit
CAGCTTCGCCGCGGTCGTGATCGACGTGAAAGAAAAACGATGGGGCTGAT12.380952380952381No Hit
CTCCTCGCGTTGCAGCGGCATGTTCTTGAGCGTCGCATCCAGCTGCTCAG12.380952380952381No Hit
GCTTGCGGTGCACAACGCCGAAGCCGTTGGAGACGTAAAACTCACCAAAC12.380952380952381No Hit
GCGCATAGGAGCCAACGCCACCGCGCGGAATGAGGGCAGCCCCGGGAGCC12.380952380952381No Hit
CCGCGATCCGCCGCGGGCGCGACATCCAGACCACTCGATCCCTCCCAAGT12.380952380952381No Hit
ACGGTCGCGTCGGGCGCGCCGGTCGGCGTCTCGTCGGTCCAGCCGGTGAG12.380952380952381No Hit
GGTCGCTGGTCCCTGAAATGTTACAGTATTTCGACATTTTTCCAAAACTT12.380952380952381No Hit
GCAGAGAGACAAATGACAAACAGCAAAAAGGAGGAGTATATCAAGTGCTC12.380952380952381No Hit
CGCCAAAGCCGGTCTTGTCGGCCTGCGCGGCAGCGGCTGCCGATGCAGCG12.380952380952381No Hit
CACCTGGTGGACGTCACTGAGCGCATCTGCATCGATGGTCGTCCCGTCAG12.380952380952381No Hit
GTCTTGGATCATATGTTGCCTTTCCAGTTTCGCGTGCACATCTGTGACTT12.380952380952381No Hit
GTATCTTCCTTCCACCTCAAAACTGTCATTTTCCCTTCCTCCCTGTTTCA12.380952380952381No Hit
GCGCTGCGCGAGGCCCGAGGAACAGTCGGGCGGGACCATGTGTGGAACCA12.380952380952381No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)