FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length140
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGACGGTCGCTTTCGTCGGCGTCTCGCTGCGCTACGCCAAGGTGT24.761904761904762No Hit
CACGGGTACAGCTGGTGATAGCCGAGCTGCACCCCAGCGTAATGAGTGTG24.761904761904762No Hit
CACTTGGAGGCCATCGTCCCGGTGTTGGACAAGGCCGCCTCGACCGCCGG24.761904761904762No Hit
GGCTTACATCAAGCAGGCGATCATCGCTGCCGCGAGTCGGAGCTACTTGC24.761904761904762No Hit
ACTAGATGGATAGCACCGCGGCGGGAGAAGGGGCCGGTGCGACGGCGTAC24.761904761904762No Hit
AGGGAGACGGTATATAACCATGTCAGGCCGACGTCGTTGGTCTGGTGTGG24.761904761904762No Hit
TCGCCGTCCCACGATCCGGACTGGTAGCTCGTGCCGTAGGCGAAGTTGTT12.380952380952381No Hit
GGACGACGCCATCCGCCTGACCCCGCCGCGCCTGATGAGCCTGGAGCAGG12.380952380952381No Hit
GCGATCTTGTCGAGACTATCGCCGAGAATATGCATATGGTCCATGGCAAC12.380952380952381No Hit
CGCCGATGTAGAGGGCCTGCCTGAAAGCCTTGTCGCTTCGTTGAAGGCGG12.380952380952381No Hit
ATTCACGCTACCGACGTGTCATGGCAAGCCATTGATTCCACCCATCTCAC12.380952380952381No Hit
NCGTGGTCTTCATTGGCCGAGCCCGACAGCGACGAGTGAATCACCACCGG12.380952380952381No Hit
GATGGACGCCGGGGTCGTGAACATCTCTAGCTTAGGCACAATCGATCCGG12.380952380952381No Hit
ATCAGGCATTGCGGCATTAGCGGCTGATATTGGACTACTGCCCTCCCGAA12.380952380952381No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGAG12.380952380952381No Hit
GTGCCGCCACATTGCCCACAAACGACTCCAGACAAGGCTTTGCCGCACTG12.380952380952381No Hit
CTCTAACTCAGCGTCGAGGTCGTCGCCGCCCTCCGAGGGATCTTGGCGAT12.380952380952381No Hit
CCAGCTACGCGAACGTGGGGCCGCAGGTGGCGCTGTCCGCGCCGGGGGGT12.380952380952381No Hit
GCGTGGGGTGACGCTTCTGCCTCGACAATAAATTGTCCGGTTTGCGCTAA12.380952380952381No Hit
GACCCCTAACGAGGTACGGGCTGCCTGGGGCGCTGGTGCTGACGCCGTCC12.380952380952381No Hit
ACTCTTGCCTGACCAGCAAGCGAAACTGAATGAGCTTGTCTCTCAGGCTG12.380952380952381No Hit
CTTTGAAACGTTTTACCGCGTACGTTTCCGCATCGACGGCATCCTCGCGC12.380952380952381No Hit
CTGTAGACAAGGTGGTGACATCCCTGGTCGGGGTGAGGGTCTGGGCTCTG12.380952380952381No Hit
CTTTGAGGGTAAATCCGTGGTCCAGCTGTCGCGCGATACCGGTATCGAAT12.380952380952381No Hit
CTCCCATGGCATGGGACTCGGTGATGACTTCCTTGACGGACTTTCCGCTG12.380952380952381No Hit
GACATCCTATTCAACACTGCCATCCTGCCCCACACCCCACATTCCCTGGC12.380952380952381No Hit
CTTCACCCCGGTCGTCGAGGCCCGCAACGCGAATGGAGCCGACAAGGTTC12.380952380952381No Hit
CTCGCACCACCGTGCTCGCGCACCTCGGTCGTCCCAAGGGTGCCGTCGAT12.380952380952381No Hit
GTGTGCTAGTGGGTCGCGGCCGGGACGGCGGCGTCCTCGCGGGCGGTGGT12.380952380952381No Hit
GAGCTGAGGAAGAGTGCTCCCTTGCTCTGTGGCAATGTCACACAAGCTGG12.380952380952381No Hit
GGTAAGATCCACAGCACAGATGGGAGAGTTAGCCTAAGAAAAAGATACAC12.380952380952381No Hit
GCGCAGCAGATAGGACATCCGAACCTGGTCGTCATTAAAGCCAGGGGTCT12.380952380952381No Hit
ATGATTTCTTGCACTATTTAAGCGGCTAGCATCATTGATCTCGGCTAATA12.380952380952381No Hit
CTCAAGGACGTCGCCGATTTCTGGGAAAAGCAGAGCTGATCCGATTAAAA12.380952380952381No Hit
CTGTTCGTGGGCGTGAAGGTGGACGAAGCGCTGTTCGAAGAGCTTGAGAC12.380952380952381No Hit
ACGTGGTCTTCATTGGCCGAGCCCGACAGCGACGAGTGAATCACCACCGG12.380952380952381No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)